Code | CSB-E13163h |
Size | 96T,5×96T,10×96T |
Price | Request a Quote or Start an on-line Chat |
Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now |
Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
Inter-assay Precision (Precision between assays): CV%<10% | ||||||
Three samples of known concentration were tested in twenty assays to assess. | ||||||
To assess the linearity of the assay, samples were spiked with high concentrations of human ADAM12 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
Sample | Serum(n=4) | |||||
1:5 | Average % | 92 | ||||
Range % | 88-96 | |||||
1:10 | Average % | 95 | ||||
Range % | 90-99 | |||||
1:20 | Average % | 85 | ||||
Range % | 81-90 | |||||
1:40 | Average % | 102 | ||||
Range % | 95-108 |
The recovery of human ADAM12 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
Sample Type | Average % Recovery | Range | ||||
Serum (n=5) | 88 | 84-93 | ||||
EDTA plasma (n=4) | 91 | 87-96 | ||||
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
|
The ELISA Kit is designed for quantitatively measuring human ADAM12 levels in samples, including serum, plasma, urine, cell culture supernates, tissue homogenates, or cell lysates. It uses the sandwich enzyme immunoassay technique in combination with the enzyme-substrate chromogenic reaction to quantify the analyte in the sample. The color develops positively to the amount of ADAM12 in samples. The color intensity is measured at 450 nm via a microplate reader.
ADAM12 belongs to the large family of ADAMs and has extracellular metalloprotease and cell-binding functions, as well as intracellular signaling capacities. ADAM12 participates in various biological processes such as signal transduction, cytoskeleton depolymerization, and micro-environmental regulation by binding and activating targeted proteins. It also plays a critical role in various types of cancers. ADAM12 is expressed at high levels in tumor tissues, and lower expression is linked to a better survival prognosis. Upregulation of ADAM12 has been detected in breast, prostate, ovarian, skin, stomach, lung, and brain cancers and can promote epithelial-mesenchymal transition (EMT), metastasis, drug resistance, and cancer stemness maintenance in numerous malignant tumors.
There are currently no reviews for this product.