Human Adenylyl cyclase-associated protein 1(CAP1) ELISA kit

Instructions
Code CSB-EL004486HU
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name CAP, adenylate cyclase-associated protein 1 (yeast)
Alternative Names Adenylyl cyclase associated protein 1 ELISA Kit; Adenylyl cyclase-associated protein 1 ELISA Kit; CAP 1 ELISA Kit; CAP ELISA Kit; CAP; adenylate cyclase associated protein 1 (yeast) ELISA Kit; CAP1 ELISA Kit; CAP1 PEN ELISA Kit; CAP1-PEN ELISA Kit; CAP1_HUMAN ELISA Kit
Abbreviation CAP1
Uniprot No. Q01518
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 25 pg/mL-1600 pg/mL
Sensitivity 6.25 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human CAP1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %92
Range %87-97
1:2Average %94
Range %90-98
1:4Average %104
Range %100-107
1:8Average %95
Range %92-99
Recovery
The recovery of human CAP1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 8884-93
EDTA plasma (n=4)9288-96
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
16002.104 2.268 2.186 2.083
8001.578 1.590 1.584 1.481
4001.163 1.152 1.158 1.055
2000.707 0.727 0.717 0.614
1000.495 0.474 0.485 0.382
500.292 0.278 0.285 0.182
250.196 0.189 0.193 0.090
00.102 0.104 0.103
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

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Target Background

Function
(From Uniprot)
Directly regulates filament dynamics and has been implicated in a number of complex developmental and morphological processes, including mRNA localization and the establishment of cell polarity.
Gene References into Functions
  1. RETN and CAP1 polymorphisms and gene expression may be potential biomarkers for breast cancer risk PMID: 29641286
  2. Significant up-regulation of PBMCs CAP1, CD36 mRNA and plasma resistin found in significant coronary artery disease, as well as in nonsignificant coronary artery disease compared to control group, indicates that resistin could be able to exert its effects stronger on cells with up-regulated CAP1 mRNA thus contributing atherosclerosis development. PMID: 28707728
  3. REVIEW: summarizes information available to date about the structural organization, regulation of functional activity of adenylyl cyclase-associated protein 1 (CAP1), and its participation in cellular processes PMID: 29534668
  4. Results identify a role for CAP1 in regulating proliferative transformation of cancer cells, with ERK signaling playing pivotal roles in mediating both cell functions. PMID: 27173014
  5. CAP1 expression is markedly increased in human glioma and that downregulation of CAP1 in tumors may serve as a treatment for glioma patients. PMID: 27432289
  6. our results indicated that CAP1 participated in the molecular pathological process of glioma PMID: 26810579
  7. a high expression of CAP1 is involved in the pathogenesis of EOC, and that the downregulation of CAP1 in tumor cells may be a therapeutic target for the treatment of patients with EOC. PMID: 25652936
  8. This study demonstrated an inverse relationship between CAP1 and MMP-9 expression, high expression of MMP-9 and low expression of CAP1 in those with chronic obstructive pulmonary disease compared with the non-chronic obstructive pulmonary disease group. PMID: 25332229
  9. CAP1 is involved in the brain metastasis in non-small cell lung cancer.CAP1 overexpression is associated with poor prognosis in non-small cell lung cancer patients. PMID: 25371324
  10. Overexpression of CAP1 in monocytes enhanced the resistin-induced increased activity of the cAMP-dependent signaling. PMID: 24606903
  11. results indicate that upregulated expression of CAP1 might contribute heavily to the metastasis of hepatocellular carcinoma. PMID: 24359721
  12. Overexpression of CAP1 is associated with breast carcinoma. PMID: 24522810
  13. CAP1 might be a potential molecular targeted therapy for surgery and immune treatment. PMID: 24509166
  14. overexpression of CAP1 in lung cancer cells, particularly at the metastatic stage, may have significant clinical implications as a diagnostic/prognostic factor for lung cancer. PMID: 23842884
  15. CAP1 depletion also led to changes in cofilin phosphorylation and localization as well as activation of focal adhesion kinase (FAK) and enhanced cell spreading. PMID: 23737525
  16. Findings suggest that CAP1 (cyclase-associated protein 1) is involved in the metastasis of esophageal squamous cell carcinoma, and increased expression may indicate a poor prognosis. PMID: 23904342
  17. plays a key role in speeding up the turnover of actin filaments by effectively recycling cofilin and actin and through its effect on both ends of actin filament PMID: 11950878
  18. Based on an analysis of 1075 beta-thalassemia alleles the CAP+1 (A-->C) mutation constituted 3.2% of north Indians. PMID: 17900295
  19. The high turnover rate of CAP1 by MMP-9, comparable to that of gelatin as the natural extracellular substrate of this enzyme, may be critical to prevent pathological conditions associated with considerable cytolysis. PMID: 18671965
  20. These studies suggest that CAP1 provides a direct link from the actin cytoskeleton to the mitochondria by functioning as an actin shuttle. PMID: 18716285
  21. The overexpression of CAP1 in pancreatic cancers is demonstrated and the involvement of CAP1 in the aggressive behavior of pancreatic cancer cells is suggested. PMID: 19188911

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Subcellular Location Cell membrane, Peripheral membrane protein
Protein Families CAP family
Database Links

HGNC: 20040

OMIM: 617801

KEGG: hsa:10487

STRING: 9606.ENSP00000361878

UniGene: Hs.370581

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