Human Afamin(AFM) ELISA kit

Instructions
Code CSB-EL001419HU
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name afamin
Alternative Names AFAM_HUMAN ELISA Kit; Afamin ELISA Kit; Afm ELISA Kit; ALB 2 ELISA Kit; ALB2 ELISA Kit; ALBA ELISA Kit; ALF ELISA Kit; Alpha albumin ELISA Kit; Alpha-Alb ELISA Kit; Alpha-albumin ELISA Kit
Abbreviation AFM
Uniprot No. P43652
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 3.9 ng/mL-250 ng/mL
Sensitivity 0.97 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human AFM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1000Average %96
Range %90-102
1:2000Average %89
Range %84-95
1:4000Average %100
Range %95-106
1:8000Average %97
Range %92-104
Recovery
The recovery of human AFM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 8993-96
EDTA plasma (n=4)10298-107
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
2502.024 2.098 2.061 1.929
1251.715 1.761 1.738 1.606
62.51.334 1.364 1.349 1.217
31.250.893 0.884 0.889 0.757
15.60.567 0.546 0.557 0.425
7.80.321 0.349 0.335 0.203
3.90.261 0.246 0.254 0.122
00.131 0.133 0.132  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 5-7 working days

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Target Data

Function Vitamin E binding protein. May transport vitamin E in body fluids under conditions where the lipoprotein system is not sufficient. May be involved in the regulation and transport of vitamin E at the blood-brain barrier.
Gene References into Functions
  1. The results showed significantly higher serum afamin levels in women with pre-eclampsia and gestational diabetes mellitus compared to healthy pregnant women. PMID: 29405972
  2. LDL apheresis moderately decreases the circulating levels of afamin parallel to lowering HDL-C and ApoA1 levels. PMID: 30247793
  3. The concentration of afamin decreases with the severity of alcoholic liver cirrhosis. PMID: 30260179
  4. Afamin binds palmitoylated serine 209 of Wnt3a, providing a structural basis how afamin solubilizes hydrophobic and poorly soluble Wnt proteins. PMID: 29153507
  5. Results provide evidence that afamin could promote glycometabolism by up-regulating the glucose metabolism key enzymes in papillary thyroid carcinoma. PMID: 27329154
  6. Data suggest that up-regulation of AFM levels in serum in women with polycystic ovary syndrome can be used as a biological markers of those women with increased risk of developing metabolic syndrome, especially those with insulin resistance. PMID: 25208973
  7. A linear increase in afamin during pregnancy may serve as basic reference for subsequent investigations of afamin in pregnancy-related disorders. PMID: 24768783
  8. Afamin levels were altered significantly in the peritoneal fluid of women with endometriosis compared with disease-free controls. PMID: 20858448
  9. Binding of bilirubin with human serum albumin was studied by absorption, fluorescence and CD spectroscopy PMID: 12063119
  10. The protein structure of afamin has been determined by homology modeling and its in vitro binding properties confirmed by docking calculations on the proposed structure. PMID: 12463752
  11. vitamin E-binding properties were confirmed; found abundant presence of this protein in plasma, extravascular fluids such as follicular, and cerebrospinal fluids PMID: 15952736
  12. afamin might be a new family member of binding/transport proteins contributing to alpha-tocopherol homeostasis at the blood-brain barrier PMID: 19046407
  13. Reduced Afamin expression is associated with ovarian cancer. PMID: 19336561

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Subcellular Location Secreted
Protein Families ALB/AFP/VDB family
Tissue Specificity High level detected in plasma but also in extravascular fluids such as follicular and cerebrospinal fluids (at protein level).
Database Links

HGNC: 316

OMIM: 104145

KEGG: hsa:173

STRING: 9606.ENSP00000226355

UniGene: Hs.168718

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