Human Afamin(AFM) ELISA kit

Code CSB-EL001419HU
Size 96T,5×96T,10×96T
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Product Details

Target Name
afamin
Alternative Names
AFAM_HUMAN ELISA Kit; Afamin ELISA Kit; Afm ELISA Kit; ALB 2 ELISA Kit; ALB2 ELISA Kit; ALBA ELISA Kit; ALF ELISA Kit; Alpha albumin ELISA Kit; Alpha-Alb ELISA Kit; Alpha-albumin ELISA Kit
Abbreviation
AFM
Uniprot No.
Species
Homo sapiens (Human)
Sample Types
serum, plasma, tissue homogenates
Detection Range
3.9 ng/mL-250 ng/mL
Sensitivity
0.97 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human AFM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1000Average %96
Range %90-102
1:2000Average %89
Range %84-95
1:4000Average %100
Range %95-106
1:8000Average %97
Range %92-104
Recovery
The recovery of human AFM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 8993-96
EDTA plasma (n=4)10298-107
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
2502.024 2.098 2.061 1.929
1251.715 1.761 1.738 1.606
62.51.334 1.364 1.349 1.217
31.250.893 0.884 0.889 0.757
15.60.567 0.546 0.557 0.425
7.80.321 0.349 0.335 0.203
3.90.261 0.246 0.254 0.122
00.131 0.133 0.132  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

The product CSB-EL001419HU is a sandwich ELISA kit developed to measure levels of human Afamin (AFM) in multiple samples, including serum, plasma, or tissue homogenates. The enzyme-substrate chromogenic reaction is also used to amplify the signal and quantify the levels of the analyte through the intensity of the colored product. The color intensity positively correlates with the amount of AFM bound in the initial step.

AFM is a human plasma vitamin E-binding glycoprotein mainly produced in the liver. It promotes vitamin E transport across the blood-brain barrier. AFM is also a tumor marker for ovarian cancer. AFM has been found strongly associated with all components of metabolic syndrome including visceral obesity and has been a potential early marker of metabolic syndrome. It also plays a role in anti-apoptotic cellular processes associated with oxidative stress and is linked to insulin resistance and other features of metabolic syndrome.

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Target Background

Function
(From Uniprot)
Functions as carrier for hydrophobic molecules in body fluids (Probable). Essential for the solubility and activity of lipidated Wnt family members, including WNT1, WNT2B, WNT3, WNT3A, WNT5A, WNT7A, WNT7B, WNT8, WNT9A, WNT9B, WNT10A and WNT10B. Binds vitamin E. May transport vitamin E in body fluids under conditions where the lipoprotein system is not sufficient. May be involved in the transport of vitamin E across the blood-brain barrier.
Gene References into Functions
  1. The results showed significantly higher serum afamin levels in women with pre-eclampsia and gestational diabetes mellitus compared to healthy pregnant women. PMID: 29405972
  2. LDL apheresis moderately decreases the circulating levels of afamin parallel to lowering HDL-C and ApoA1 levels. PMID: 30247793
  3. The concentration of afamin decreases with the severity of alcoholic liver cirrhosis. PMID: 30260179
  4. Afamin binds palmitoylated serine 209 of Wnt3a, providing a structural basis how afamin solubilizes hydrophobic and poorly soluble Wnt proteins. PMID: 29153507
  5. Results provide evidence that afamin could promote glycometabolism by up-regulating the glucose metabolism key enzymes in papillary thyroid carcinoma. PMID: 27329154
  6. Data suggest that up-regulation of AFM levels in serum in women with polycystic ovary syndrome can be used as a biological markers of those women with increased risk of developing metabolic syndrome, especially those with insulin resistance. PMID: 25208973
  7. A linear increase in afamin during pregnancy may serve as basic reference for subsequent investigations of afamin in pregnancy-related disorders. PMID: 24768783
  8. Afamin levels were altered significantly in the peritoneal fluid of women with endometriosis compared with disease-free controls. PMID: 20858448
  9. Binding of bilirubin with human serum albumin was studied by absorption, fluorescence and CD spectroscopy PMID: 12063119
  10. The protein structure of afamin has been determined by homology modeling and its in vitro binding properties confirmed by docking calculations on the proposed structure. PMID: 12463752
  11. vitamin E-binding properties were confirmed; found abundant presence of this protein in plasma, extravascular fluids such as follicular, and cerebrospinal fluids PMID: 15952736
  12. afamin might be a new family member of binding/transport proteins contributing to alpha-tocopherol homeostasis at the blood-brain barrier PMID: 19046407
  13. Reduced Afamin expression is associated with ovarian cancer. PMID: 19336561

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Subcellular Location
Secreted.
Protein Families
ALB/AFP/VDB family
Tissue Specificity
High level detected in plasma but also in extravascular fluids such as follicular and cerebrospinal fluids (at protein level).
Database Links

HGNC: 316

OMIM: 104145

KEGG: hsa:173

STRING: 9606.ENSP00000226355

UniGene: Hs.168718

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