Human Angiotensin Ⅰ Converting Enzyme,ACEⅠ ELISA Kit

Code CSB-E11269h
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P12821
Lead Time 3-5 working days
Abbreviation ACE
Target Name angiotensin I converting enzyme (peptidyl-dipeptidase A) 1
Alias ACE1, CD143, DCP, DCP1, MGC26566, MVCD3, CD143 antigen|angiotensin I converting enzyme 1|angiotensin converting enzyme, somatic isoform|carboxycathepsin|dipeptidyl carboxypeptidase 1|kininase II|pep
Species Homo sapiens (Human)
Sample Types serum, plasma, cell culture supernates, tissue homogenates
Detection Range 18.75 pg/mL-1200 pg/mL
Sensitivity 4.7 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Neuroscience
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This gene encodes an enzyme involved in catalyzing the conversion of angiotensin I into a physiologically active peptide angiotensin II. Angiotensin II is a potent vasopressor and aldosterone-stimulating peptide that controls blood pressure and fluid-electrolyte balance. This enzyme plays a key role in the renin-angiotensin system. Many studies have associated the presence or absence of a 287 bp Alu repeat element in this gene with the levels of circulating enzyme or cardiovascular pathophysiologies. Two most abundant alternatively spliced variants of this gene encode two isozymes - the somatic form and the testicular form that are equally active. Multiple additional alternatively spliced variants have been identified but their full length nature has not been determined.
HGNC 2707
RGD 2493
MGI 87874
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human ACEⅠ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:200Average %97
Range %92-104
1:400Average %91
Range %82-98
1:800Average %103
Range %99-111
1:1600Average %87
Range %82-96
Recovery
The recovery of human ACEⅠ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9085-98
EDTA plasma (n=4)112105-117
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
12002.844 2.752 2.798 2.663
6002.536 2.382 2.459 2.324
3001.916 1.985 1.951 1.816
1501.390 1.355 1.373 1.238
750.715 0.764 0.740 0.605
37.50.386 0.375 0.381 0.246
18.750.222 0.239 0.231 0.096
00.132 0.137 0.135
References
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Function Converts angiotensin I to angiotensin II by release of the terminal His-Leu, this results in an increase of the vasoconstrictor activity of angiotensin. Also able to inactivate bradykinin, a potent vasodilator. Has also a glycosidase activity which releases GPI-anchored proteins from the membrane by cleaving the mannose linkage in the GPI moiety.
Involvement in disease Ischemic stroke (ISCHSTR); Renal tubular dysgenesis (RTD); Microvascular complications of diabetes 3 (MVCD3); Intracerebral hemorrhage (ICH)
Subcellular Location Angiotensin-converting enzyme, soluble form: Secreted, SUBCELLULAR LOCATION: Cell membrane, Single-pass type I membrane protein, Cytoplasm
Protein Families Peptidase M2 family
Tissue Specificity Ubiquitously expressed, with highest levels in lung, kidney, heart, gastrointestinal system and prostate. Isoform Testis-specific is expressed in spermatocytes and adult testis.
Database Links

HGNC: 2707

OMIM: 106180

KEGG: hsa:1636

STRING: 9606.ENSP00000290866

UniGene: Hs.298469

Pathway Renin-angiotensin system

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