Human Annexin Ⅲ(ANX-Ⅲ) ELISA Kit

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Target Background

Name: Human Annexin Ⅲ(ANX-Ⅲ) ELISA Kit
Brand: CUSABIO
SKU: CSB-E12157h

Product Details

Target Name

annexin A3

Alternative Names

2-cyclic phosphate 2-phosphohydrolase ELISA Kit; 35-alpha calcimedin ELISA Kit; Annexin A3 ELISA Kit; Annexin III ELISA Kit; Annexin-3 ELISA Kit; ANX3 ELISA Kit; ANXA 3 ELISA Kit; Anxa3 ELISA Kit; ANXA3_HUMAN ELISA Kit; Calcimedin 35 alpha ELISA Kit; Inositol 1 2 cyclic phosphate 2 phosphohydrolase ELISA Kit; Inositol 1 ELISA Kit; Lipocortin III ELISA Kit; OTTHUMP00000160701 ELISA Kit; OTTHUMP00000219237 ELISA Kit; OTTHUMP00000219238 ELISA Kit; PAP III ELISA Kit; PAP-III ELISA Kit; Placental anticoagulant protein III ELISA Kit

Abbreviation

ANXA3

Uniprot No.

P12429

Species

Homo sapiens (Human)

Sample Types

serum, plasma, cell culture supernates, tissue homogenates, cell lysates

Detection Range

0.78 ng/mL-50 ng/mL

Sensitivity

0.195 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Signal Transduction

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human ANX-Ⅲin various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	92-101
1:2	Average %	90
	Range %	82-96
1:4	Average %	101
	Range %	99-104
1:8	Average %	87
	Range %	82-99

Recovery

The recovery of human ANX-Ⅲ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	82-96
EDTA plasma (n=4)	95	89-100

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
50	2.425	2.550	2.488	2.370
25	2.010	2.174	2.092	1.974
12.5	1.266	1.363	1.315	1.197
6.25	0.559	0.597	0.578	0.460
3.12	0.275	0.261	0.268	0.150
1.56	0.192	0.186	0.189	0.071
0.78	0.131	0.140	0.136	0.018
0	0.122	0.113	0.118

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Description

This Human ANXA3 ELISA Kit was designed for the quantitative measurement of Human ANXA3 protein in serum, plasma, cell culture supernates, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 0.78 ng/mL-50 ng/mL and the sensitivity is 0.195 ng/mL .

Code	CSB-E12157h
Size	96T,5×96T,10×96T
Price	Request a Quote
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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.