Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

ANXA3

Uniprot No.

P12429

Research Area

Signal Transduction

Alternative Names

2-cyclic phosphate 2-phosphohydrolase; 35-alpha calcimedin; Annexin A3; Annexin III; Annexin-3; ANX3; ANXA 3; Anxa3; ANXA3_HUMAN; Calcimedin 35 alpha; Inositol 1 2 cyclic phosphate 2 phosphohydrolase ; Inositol 1; Lipocortin III; OTTHUMP00000160701; OTTHUMP00000219237; OTTHUMP00000219238; PAP III; PAP-III; Placental anticoagulant protein III

Species

Homo sapiens (Human)

Source

E.coli

Expression Region

5-322aa

Target Protein Sequence

WVGHRGTVRDYPDFSPSVDAEAIQKAIRGIGTDEKMLISILTERSNAQRQLIVKEYQAAYGKELKDDLKGDLSGHFEHLMVALVTPPAVFDAKQLKKSMKGAGTNEDALIEILTTRTSRQMKDISQAYYTVYKKSLGDDISSETSGDFRKALLTLADGRRDESLKVDEHLAKQDAQILYKAGENRWGTDEDKFTEILCLRSFPQLKLTFDEYRNISQKDIVDSIKGELSGHFEDLLLAIVNCVRNTPAFLAERLHRALKGIGTDEFTLNRIMVSRSEIDLLDIRTEFKKHYGYSLYSAIKSDTSGDYEITLLKICGGD
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

39.9kDa

Protein Length

Partial

Tag Info

N-terminal 6xHis-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

In e.coli cells, the generation of recombinant Human ANXA3 protein involves cloning a DNA fragment encoding the Human ANXA3 protein (5-322aa) into a plasmid vector, which is then transferred into the e.coli cells. Positive cells are selected, cultured, and induced to express the ANXA3 protein. A N-terminal 6xHis tag is attached to the protein. Lysis of the cells allows for the harvest of the recombinant Human ANXA3 protein. The collected recombiant Human ANXA3 protein is subjected to affinity purification and is identified using SDS-PAGE and subsequent staining of the gel with Coomassie Brilliant Blue. Its purity is greater than 90%.

Target Background

Function

Inhibitor of phospholipase A2, also possesses anti-coagulant properties. Also cleaves the cyclic bond of inositol 1,2-cyclic phosphate to form inositol 1-phosphate.

Gene References into Functions

ANXA3 levels in urine show clinically significant correlation with real tumor volumes. PMID: 28703915
The down-regulation of Annexin A3 by siRNA inhibited the invasion and epithelial-mesenchymal transition of colorectal cancer cells through the up-regulation of p53. PMID: 29224019
ANXA3 plays important roles in gastric cancer carcinogenesis and metastasis. PMID: 27894078
The findings implicate the expression of ANXA3 with the natural progression of breast cancer and associate it with increased lymphatic metastasis. The study validates the use of ANXA3 as a potential prognosis biomarker for breast cancer. PMID: 28497041
we identified ANXA3 as a regulator of hepatitis C virus maturation and egress PMID: 27653686
ANXA3 role in the proliferation and invasion of breast cancer cells. PMID: 27878264
Results identified potential variant in ANXA3 gene [chr4, c.C820T(p.R274*)] in a large family with an unknown equinus deformity, which could lead to a three-dimensional conformational change. PMID: 27475959
Anxa3 knockdown inhibited the growth, migration, invasion, and metastasis of lung adenocarcinoma. PMID: 27995049
These results suggest that the iEA index or a combination of polymorphisms in EGFR and ANXA3 may serve as predictive factors of drug response, and therefore could be useful for optimal selection of chemotherapy regimens. PMID: 26475168
ANX A3 has roles as a mammary biomarker, regulator and therapeutic target in breast cancer PMID: 26093083
Our results suggest that ANXA3 can serve as a novel diagnostic biomarker and that the inhibition of ANXA3 may be a viable therapeutic option for the treatment of CD133+ liver-CSC-driven HCC. PMID: 26095609
Findings reveal that ANXA3 might play an important role in hepatocellular carcinoma progression and chemoresistance. PMID: 24375474
findings suggest that ANXA3 plays a role in HCC CSC/CIC maintenance, and that ANXA3 may represent a potential CSC/CIC-specific therapeutic target for improving the treatment of HCC. PMID: 25267273
Two different antigenic variants of ANXA3 are present in post-DRE urines and their clinical significance for diagnosis of prostate cancer should be further investigated. PMID: 24954692
Expression of annexin A3 was increased in gastric cancer compared with that in normal gastric tissues. Annexin A3 expression was significantly associated with tumor volume and TNM stage. PMID: 24815437
Annexin A3 was upregulated in gastric cancer cells. Deletion of endogenous Annexin A3 significantly inhibited gastric cancer cell proliferation, migration, and invasion. PMID: 24824926
Low ANXA3 expression is associated with radioresistance in nasopharyngeal carcinoma. PMID: 23464856
Annexin A3 expression correlates with tumor size and lymph node metastasis.Annexin A3 might be regulated apoptosis by affecting Bcl-2/Bax balance.Annexin A3 was an independent prognostic factor in breast cancer. PMID: 23631820
The expression change of Anxa3 can be utilized as a potential indicator for the development, invasion, metastasis and drug resistance of tumors. PMID: 23011854
the association of multi-drug resistance with ANXA3, one of the highly expressed proteins in BEL7402/5-FU-resistant hepatoma cell line, was verified PMID: 22189913
annexin A3 secretion may be associated with exocytosis and the release of exosomes PMID: 21435174
Decreased expression of ANXA3 in papillary thyroid cancer supports the idea that ANXA3 may be an effective marker of microcarcinoma, and a negative predictor of papillary thyroid cancer progression. PMID: 21137070
AnnexinA3 plays an important role in the initiation and progression of human gallbladder cancer. PMID: 21055154
two spliced isoforms of Annexin A3 are expressed differently in human renal cortex and renal-cell carcinoma PMID: 20167856
Annexin A3 could be a target for therapeutic intervention and may also serve as a biomarker for drug resistance in ovarian cancer patients. PMID: 20103635
ANXA3 is a novel angiogenic factor that induces vascular endothelial growth factor production through the hypoxia-inducible factor-1 pathway PMID: 16236264

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Protein Families

Annexin family

Database Links

HGNC: 541

OMIM: 106490

KEGG: hsa:306

STRING: 9606.ENSP00000264908

UniGene: Hs.480042

Code	CSB-RP171494h
Abbreviation	Recombinant Human ANXA3 protein, partial
MSDS	MSDS Datasheet
Size	$224
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Human Annexin A3 (ANXA3), partial

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