Human Anterior gradient protein 3 homolog(AGR3) ELISA kit

Instructions
Code CSB-EL001459HU
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name anterior gradient homolog 3 (Xenopus laevis)
Alternative Names AG-3 ELISA Kit; AG3 ELISA Kit; Agr3 ELISA Kit; AGR3_HUMAN ELISA Kit; Anterior gradient 3; xenopus; homolog of ELISA Kit; Anterior gradient homolog 3 ELISA Kit; Anterior gradient homolog 3 (Xenopus laevis) ELISA Kit; anterior gradient protein 3 ELISA Kit; Anterior gradient protein 3 homolog ELISA Kit; BCMP11 ELISA Kit; Breast cancer membrane protein 11 ELISA Kit; Gm888 ELISA Kit; hAG 3 ELISA Kit; hAG-3 ELISA Kit; HAG3 ELISA Kit; OTTHUMP00000158512 ELISA Kit; PDIA18 ELISA Kit; Protein disulfide isomerase family A member 18 ELISA Kit
Abbreviation AGR3
Uniprot No. Q8TD06
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 0.156 ng/mL-10 ng/mL
Sensitivity 0.039 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cancer
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human AGR3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 85  
Range % 80-89  
1:2 Average % 91  
Range % 86-95  
1:4 Average % 94  
Range % 90-98  
1:8 Average % 97  
Range % 92-101  
Recovery
The recovery of human AGR3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 90 85-95  
EDTA plasma (n=4) 94 89-99  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
10 2.713 2.662 2.688 2.515  
5 2.376 2.431 2.404 2.231  
2.5 1.934 2.001 1.968 1.795  
1.25 1.509 1.582 1.546 1.373  
0.625 0.974 1.020 0.997 0.824  
0.312 0.564 0.559 0.562 0.389  
0.156 0.296 0.279 0.288 0.115  
0 0.178 0.167 0.173    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

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Target Data

Function Required for calcium-mediated regulation of ciliary beat frequency and mucociliary clearance in the airway. Might be involved in the regulation of intracellular calcium in tracheal epithelial cells.
Gene References into Functions
  1. Results show that AGR3 was highly upregulated in low-grade serous carcinoma (LGSC) compared to high-grade serous carcinoma (HGSC). It can be use along with p53 for differential diagnosis of HGSC and LGSC. PMID: 29620196
  2. transcriptionally activated in hepatitis C virus-infected cells by HCV NS5A PMID: 25868611
  3. Data show the potential usability of anterior gradient homolog 3 (AGR3) and anterior gradient homolog 2 (AGR2) as biomarkers for blood-based early detection of breast cancer. PMID: 25875093
  4. AGR3 is a specialized member of the PDI family that plays an unexpected role in the regulation of CBF and mucociliary clearance in the airway. PMID: 25751668
  5. Relying on available data and in silico analyses, study shows that AGR2 and AGR3 proteins are co-expressed or uncoupled in context-dependent manners in diverse carcinomas and healthy tissues. PMID: 25666661
  6. AGR3 was is predominantly expressed in liver cholangiocytes but not present in liver hepatocytes, making this protain a good marker for differential diagnosis of intrahepatic cholangiocarcinoma PMID: 24747240
  7. AGR3 expression in ovarian cancer is independent of oestrogen-receptor expression. PMID: 22361111
  8. AGR3 could serve as a prognostic marker for survival in patients with low grade and high grade serous ovarian carcinomas. PMID: 21451362
  9. hAG-2 and hAG-3, human homologues of genes involved in differentiation, are associated with oestrogen receptor-positive breast tumours and interact with metastasis gene C4.4a and dystroglycan [hAG-3 protein] PMID: 12592373
  10. specific induction of Hag2 and Hag3 during hormone-induced breast carcinomas further support developmental specificity for the PDI/ERp family members PMID: 15935701

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Subcellular Location Endoplasmic reticulum
Protein Families AGR family
Tissue Specificity Expressed in the lung, in the ciliated cells of the airway epithelium (PubMed:25751668). Expression increased with differentiation of airway epithelial cells (PubMed:25751668). Not detected in the mucous cells (PubMed:25751668). Expressed in ciliated cell
Database Links

HGNC: 24167

OMIM: 609482

KEGG: hsa:155465

STRING: 9606.ENSP00000308606

UniGene: Hs.100686

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