Human Apelin receptor(APLNR) ELISA kit

Instructions
Code CSB-EL001910HU
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name apelin receptor
Alternative Names AGTR L1 ELISA Kit; AGTRL 1 ELISA Kit; AGTRL1 ELISA Kit; Angiotensin II receptor like 1 ELISA Kit; Angiotensin receptor like 1 ELISA Kit; Angiotensin receptor-like 1 ELISA Kit; Apelin receptor ELISA Kit; APJ (apelin) receptor ELISA Kit; APJ ELISA Kit; APJ receptor ELISA Kit; APJ_HUMAN ELISA Kit; APJR ELISA Kit; APLNR ELISA Kit; B78 ELISA Kit; FLJ90771 ELISA Kit; G protein coupled receptor APJ ELISA Kit; G-protein coupled receptor APJ ELISA Kit; G-protein coupled receptor HG11 ELISA Kit; GPCR34 ELISA Kit; HG 11 ELISA Kit; HG11 ELISA Kit; HG11 orphan receptor ELISA Kit; MGC45246 ELISA Kit; MSR ELISA Kit; msr/apj ELISA Kit
Abbreviation APLNR
Uniprot No. P35414
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 23.5 pg/mL-1500 pg/mL
Sensitivity 5.8 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Neuroscience
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human APLNR in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 91  
Range % 88-95  
1:2 Average % 95  
Range % 90-100  
1:4 Average % 97  
Range % 94-103  
1:8 Average % 101  
Range % 96-105  
Recovery
The recovery of human APLNR spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 90 87-94  
EDTA plasma (n=4) 96 94-101  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
1500 2.312 2.189 2.251 2.081  
750 1.494 1.455 1.475 1.305  
375 1.026 0.994 1.010 0.840  
187.5 0.655 0.688 0.672 0.502  
94 0.448 0.442 0.445 0.275  
47 0.303 0.292 0.298 0.128  
23.5 0.241 0.232 0.237 0.067  
0 0.163 0.177 0.170    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

Target Data

Function Receptor for apelin receptor early endogenous ligand (APELA) and apelin (APLN) hormones coupled to G proteins that inhibit adenylate cyclase activity
Gene References into Functions
  1. these data... suggest that APLNR alteration neither by mutation nor expression loss is common in colorectal and prostate cancer PMID: 29573866
  2. leptin, leptin receptor and apelin receptor genes are associated with susceptibility to coronary artery disease and hypertension PMID: 29883719
  3. Studies indicate that the Elabela-APJ (apelin receptor) axis may protect against pressure overload-induced heart failure. PMID: 29036564
  4. The high APJ expression has significantly higher rates of tumor invasion, local lymph node, and distant metastasis (all p < 0.001). PMID: 28514205
  5. The mutation of polymorphism rs10501367 in APJ gene decreased risk of hypertension in Chinese males. PMID: 29800734
  6. these observations suggest apelin-APJ signaling in hepatocytes functions to protect against lipid accumulation in liver through two signaling pathways, that is, via AMPK activation and PPARalpha induction. PMID: 28821440
  7. 916 ischemic stroke patients were recruited for the study. For age at ischemic stroke onset, no significant association was identified with the variant rs9943582 of APLNR in any genetic model. In addition, the variant was not strongly associated with recurrence and death risk of ischemic stroke, as shown by the results. PMID: 28648959
  8. the expression of APLNR (APJ/AGTRL1), the only known receptor for apelin, is predominantly restricted to the endothelial cells. PMID: 28904225
  9. Studies suggest that apelin/APJ system may be applied to the treatment of cancers by regulating apoptosis, which may play a vital role in anticancer therapy [review]. PMID: 28407045
  10. Crystal structure of human APJR shows structural basis for apelin recognition and binding PMID: 28528775
  11. mutation of promoter polymorphism rs7119375 in APJ gene was significantly associated with elevated SBP and increased hypertension risk in Chinese women. PMID: 27863393
  12. The APLNR A445C polymorphism was not associated with diabetic retinopathy in a sample of the Tunisian population, but the CC genotype carrier patients with DR had a high total cholesterol concentration. PMID: 28182349
  13. APLN and APLNR are present in human ovarian cells and APLN increases IGF1-induced steroidogenesis in granulosa cells through an increase in HSD3B protein expression and activation of the MAPK3/1 and Akt pathways. Therefore, APLN and APLNR may play a role in human follicular development and the pathogenesis of PCOS. PMID: 27683264
  14. These results demonstrate a new pharmacologic property of protamine-blockade of APJ-that could explain some adverse effects observed in protamine-treated patients, and that the established antiangiogenic activity of protamine would rely on APJ antagonism. PMID: 28242772
  15. The apelin/APLNR axis regulates cholangiocarcinoma cell proliferation and tumor angiogenesis. PMID: 27894959
  16. Data suggest that the differential expression of Apelin and Apelin receptor APJ yields a "self-generated" gradient mechanisms that accelerates the extension of the sprout. PMID: 27828952
  17. The aim of this study was to evaluate if polymorphisms of APLN and APLNR genes may play a role as susceptibility markers for hypertension in a group of Mexican-Mestizo patients. PMID: 27450650
  18. review of the important role of the Apelin/APJ system in pathological angiogenesis PMID: 28025030
  19. The optimal gene-gene interaction model for both males and females with regard to hypertension was apelin rs3761581-apelin rs3115757-APJ rs7119375. In conclusion, gene polymorphisms of the apelin-APJ system are associated with susceptibility to hypertension. PMID: 27338090
  20. Up-regulation of apelin and APJ expression plays an important role in cardioprotection from myocardial ischemia/reperfusion injury. PMID: 27854125
  21. These findings suggested that Apelin/APLNR signaling may be used as a potential therapeutic target for hypoxic/ischemic injury. PMID: 26676468
  22. The expressions of apelin and APJ are significantly augmented by hypoxia through the hypoxia-inducible factor-1 alpha (HIF-1alpha) signaling pathway. PMID: 26436483
  23. obese (especially diabese) youngsters demonstrated lower serum apelin levels; the G212A polymorphism of the APLNR gene was found to exert a favourable effect on circulating apelin levels in childhood obesity PMID: 25060841
  24. Apelin-APJ system is a novel neurohormonal pathway, with studies to date suggesting that it may be of pathophysiologic relevance in Heart failure. PMID: 25795508
  25. apelin is produced by arterial endothelial cells (ECs) during embryogenesis, induces chemotaxis of venous ECs, and promotes the production of secreted Frizzled-related protein 1 by apelin receptor(+) ECs PMID: 25920569
  26. This minireview outlines key (patho)physiological actions of the AR, addresses what is known about signal transduction downstream of AR activation PMID: 25275559
  27. built 3 homology 3-D models of the ApelinR and revealed the conservation at the bottom of the apelin binding site of a hydrophobic cavity in which the C-terminal Phe of pE13F was embedded PMID: 25359495
  28. ERG and APLNR are essential for endothelial homeostasis in venules in the lung and that perturbation in ERG-APLNR signaling is crucial for the development of pulmonary veno-occlusive disease. PMID: 25062690
  29. AGTRL1 genetic polymorphisms might contribute to the development of hypertension independently and/or through complex interaction. PMID: 24465893
  30. serine 348 on the apelin receptor is a novel regulatory phosphorylation site in apelin-13-induced G protein-independent biased signaling PMID: 25271156
  31. APJ and B1R can form heterodimers in transfected HEK293 cells and activations of APJ and B1R could up-regulate eNOS phosphorylation PMID: 24686079
  32. REVIEW: Growing evidence shows that apelin/APJ system functions as a critical mediator of cardiovascular homeostasis and is involved in the pathophysiology of cardiovascular diseases. PMID: 24055369
  33. Apelin-APJ is overexpressed, and works as a signal for arteriogenesis in HCC. PMID: 25275024
  34. functional role of the apelin--APJ system likely in early gestation PMID: 23844873
  35. Apelin may be associated with proliferative vasculopathy in systemic sclerosis. PMID: 22112232
  36. Introduce a new correlative NMR spectroscopy and computational biochemistry methodology and demonstrate its utility in providing some of the first high-resolution structural information for a peptide-activated apelin receptor transmembrane domain. PMID: 23438363
  37. Interactive effects of genetic defects in apelin/APJ pathway might confer a potential risk in Chinese hypertensive patients. PMID: 23226564
  38. Apelin/APJ pathway serves as a critical intermediary that links statin to its pleiotropic effects in regulating endothelial gene targets and function. PMID: 22995518
  39. APELIN promotes hematopoiesis from human embryonic stem cells. PMID: 22611158
  40. the novel peptide apelin and its receptor APJ can induce the morphological and functional maturation of blood vessels in tumors PMID: 22037214
  41. There was an increased frequency of the G212 allele of AJP receptor in patients with hypertension in respect to patients without hypertension. PMID: 22109355
  42. hypoxia and inflammatory factors could play a major role in the activation of the hepatic apelin system leading to angiogenic and fibroproliferative response occurring in chronic liver disease. PMID: 21450694
  43. Disruption of apelin-APJ signaling can exacerbate pulmonary hypertension mediated by decreased activation of AMP-activated kinase and eNOS. PMID: 21233449
  44. Single nucleotide polymorphisms of the APLNR gene were significantly associated with diastolic blood pressure and mean arterial blood pressure responses to low-sodium intervention. PMID: 20125035
  45. found robust haplotype-based and haplotype-phenotype associations of four well characterized polymorphisms in apelin-AGTRL1 system with hypertension and related phenotypes PMID: 20485192
  46. These findings bring new insights into apelin receptor activation and show that Phe(255) and Trp(259), by interacting with the C-terminal Phe of the pyroglutamyl form of apelin 13 (pE13F) or K17F, are crucial for apelin receptor internalization. PMID: 20675385
  47. Our study indicates that genetic variation of APLN-APJ and ACE2 may influence BP response to potassium intake. PMID: 20224560
  48. The apelin/APJ system may be involved in retinal neovascularization during the development of proliferative diabetic retinopathy. PMID: 20220056
  49. These observations revealed a novel ligand-dependent targeting of the apelin receptor to beta-arrestin-associated and -dissociated trafficking pathways and a role for different Rab proteins to direct these pathways. PMID: 20353754
  50. Endothelial expression of apelin receptors is observed during the embryonic formation of blood vessels. The apelin system is both involved in physiological angiogenesis and pathological neoangiogenesis. Review. PMID: 19527631
  51. While apelin/APJ myocardial expression decreases, apelin plasma levels increase in left ventricular hypertrophy PMID: 20055692
  52. No association between APLNR mRNA expression and gestational diabetes. PMID: 19926159
  53. Aortic valve stenosis is characterized by an up-regulation of the apelin-APJ signaling pathway. PMID: 20099713
  54. internalization and recycling, in stably expressing indicator cells, human neurons, primary CNS microvascular endothelial cells, and astrocytes PMID: 12667811
  55. results demonstrate that apelin receptor exhibits nuclear localization in brain, distinct cell-dependent mechanisms for the nuclear transport of apelin receptors, and disruption of nuclear signal sequence disrupts its nuclear translocation PMID: 14645236
  56. second 10 residues of the N-terminal domain of APJ are critical for association with apelin, while the first 20 amino acids play an important role in supporting cell-cell fusion mediated by HIV-1 gp120 PMID: 14675627
  57. APJ exerts the hypotensive effect in vivo and plays a counterregulatory role against the pressor action of angiotensin II PMID: 15087458
  58. reported the presence of APJ receptor-like immunoreactivity on cardiomyocytes, vascular smooth muscle cells and endothelial cells and the presence of apelin-like immunoreactivity in secretory vesicles of vascular endothelial cells PMID: 15664671
  59. apelin and APJ are expressed in preeclamptic placentas and may have a role in development of preeclampsia PMID: 17128405
  60. The results indicate that the SNP in the AGTRL1 gene is associated with the susceptibility to brain infarction. PMID: 17309882
  61. Apelin and APJ expression is highly upregulated in microvascular proliferations of brain tumors such as malignant gliomas. PMID: 17412318
  62. In conclusion, the ERK1/2, but not p38 MAPK pathway is activated by apelin-13 through coupling of human APJ to Gi2-protein, which contributes to cellular responses. PMID: 18401529
  63. Nuclear magnetic resonance and circular dichroism spectroscopy results are presented for a variety of apelin peptides at both physiological and low temperatures allowing determination of the bioactive structure of apelin. PMID: 19123778
  64. genetic variation within apelin and AGTRL1 likely contributes to essential hypertension, BMI and the onset age of hypertension PMID: 19307984
  65. AGTRL1 polymorphism is not associated with coronary artery disease. PMID: 19680269

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Subcellular Location Cell membrane
Protein Families G-protein coupled receptor 1 family
Tissue Specificity Expressed in heart, brain, kidney, stomach, spleen, thymus, lung, ovary, small intestine and colon, adipose tissues and pancreas (PubMed:8294032, PubMed:25639753). Expressed in glial cells, astrocytes and neuronal subpopulations (PubMed:8294032). Expresse
Database Links

HGNC: 339

OMIM: 600052

KEGG: hsa:187

STRING: 9606.ENSP00000257254

UniGene: Hs.438311

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