Code | CSB-E09040h |
Size | 96T,5×96T,10×96T |
Price | Request a Quote or Start an on-line Chat |
Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now |
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
To assess the linearity of the assay, samples were spiked with high concentrations of human AA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
This human arachidonic acid (AA) ELISA Kit is suitable for qualitatively determining human arachidonic acid concentrations in serum, plasma, cell culture supernates, or tissue homogenates in vitro. Arachidonic acid participates in important biological processes such as growth, and development, and is a precursor of numerous lipid mediators. When obtained from food such as poultry, animal organs & meat, fish, seafood, and eggs, arachidonic acid is incorporated in phospholipids in the cells' cytosol, adjacent to the endoplasmic reticulum membrane that is studded with the proteins necessary for phospholipid synthesis and their allocation to the diverse biological membranes. Arachidonic acid can also be obtained by desaturation and chain elongation of the plant-rich essential fatty acid, linoleic acid.
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate has been pre-coated with an antibody specific to AA. Standards or samples are added to the appropriate microtiter plate wells with an HRP-conjugated AA. The competitive inhibition reaction is launched between HRP-conjugated AA and AA in samples. A substrate solution is added to the wells and the color develops oppositely to the amount of AA in the sample. The color development is stopped and the intensity of the color is measured.
There are currently no reviews for this product.