Human Arachidonic Acid,AA ELISA Kit

Code CSB-E09040h
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase in six months till depleted. Apply now

Product Details

Target Name
Arachidonic Acid,AA
Alternative Names
Homo sapiens (Human)
Sample Types
serum, plasma, cell culture supernates, tissue homogenates
Detection Range
78.125 ng/mL-5000 ng/mL
69.748 ng/mL
Assay Time
Sample Volume
Detection Wavelength
450 nm
Research Area
Assay Principle


Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.




To assess the linearity of the assay, samples were spiked with high concentrations of human AA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


Typical Data


These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


and FAQs
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

This human arachidonic acid (AA) ELISA Kit is suitable for qualitatively determining human arachidonic acid concentrations in serum, plasma, cell culture supernates, or tissue homogenates in vitro. Arachidonic acid participates in important biological processes such as growth, and development, and is a precursor of numerous lipid mediators. When obtained from food such as poultry, animal organs & meat, fish, seafood, and eggs, arachidonic acid is incorporated in phospholipids in the cells' cytosol, adjacent to the endoplasmic reticulum membrane that is studded with the proteins necessary for phospholipid synthesis and their allocation to the diverse biological membranes. Arachidonic acid can also be obtained by desaturation and chain elongation of the plant-rich essential fatty acid, linoleic acid.

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate has been pre-coated with an antibody specific to AA. Standards or samples are added to the appropriate microtiter plate wells with an HRP-conjugated AA. The competitive inhibition reaction is launched between HRP-conjugated AA and AA in samples. A substrate solution is added to the wells and the color develops oppositely to the amount of AA in the sample. The color development is stopped and the intensity of the color is measured.

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