Sheep arachidonic Acid(AA) ELISA kit

Code CSB-EQ027590SH
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
arachidonic Acid(AA)
Abbreviation
AA
Species
Ovis aries (Sheep)
Sample Types
serum, plasma
Detection Range
15.625 ng/mL-1000 ng/mL
Sensitivity
13.797 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Biochemicals
Assay Principle
quantitative
Measurement
Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of sheep AA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:100 Average % 96
Range % 89-100
1:200 Average % 90
Range % 85-95
1:400 Average % 100
Range % 94-104
1:800 Average % 105
Range % 97-110
Recovery
The recovery of sheep AA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 102 95-106
EDTA plasma (n=4) 102 96-107
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average
1000 0.122 0.120 0.121
500 0.166 0.158 0.162
250 0.232 0.241 0.237
125 0.337 0.344 0.341
62.5 0.528 0.510 0.519
31.25 0.941 0.955 0.948
15.625 1.471 1.522 1.497
0 2.326 2.419 2.373
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Sheep arachidonic Acid (AA) ELISA Kit was designed for the quantitative measurement of Sheep arachidonic Acid (AA) protein in serum, plasma. It is a Competitive ELISA kit, its detection range is 15.625 ng/mL-1000 ng/mL and the sensitivity is 13.797 ng/mL.

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