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| Code | CSB-E13426h |
| Size | 96T,5×96T,10×96T |
| Price | Request a Quote or Start an on-line Chat |
| Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now |
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
| To assess the linearity of the assay, samples were spiked with high concentrations of human CTRP3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 88 | ||||
| Range % | 81-95 | |||||
| 1:2 | Average % | 92 | ||||
| Range % | 90-102 | |||||
| 1:4 | Average % | 96 | ||||
| Range % | 91-100 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 89-102 | |||||
| The recovery of human CTRP3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 85-100 | ||||
| EDTA plasma (n=4) | 101 | 90-110 | ||||
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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This human CTRP3 ELISA kit employs the quantitative sandwich enzyme immunoassay technique to measure the levels of human CTRP3 in multiple samples, including serum, plasma, or cell culture supernates. Antibody specific for CTRP3 has been pre-coated onto the microplate. Standards and samples are pipetted into the wells and any CTRP3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated CTRP3 antibody is added to the wells. After washing, avidin conjugated HRP is added to the wells, forming an antibody-antigen-enzyme-labeled antibody complex. Following a wash to remove any unbound HRP-avidin, the TMB substrate solution is added to the wells, and the color develops into blue. The color changes from blue to yellow after the addition of stop solution into the wells. The color intensity is in proportion to the amount of CTRP3 bound in the initial step.
CTRP3 (C1QTNF3) is one of the anti-inflammatory adipocytokines and inversely correlates with pro-inflammatory cytokines such as IL-6, TNF, and CRP. It is involved in various physiological and pathological processes, including glucose/lipid metabolism, inflammation, proliferation, apoptosis, vascular calcification, fibrosis, ischemic injury, and cardiovascular protection. CTRP3 also influences some specific inflammatory disorders, such as immunoglobulin A nephropathy and inflammatory bowel disease. Apart from its role in inflammation, CTRP3 affects the differentiation and adipogenesis of adipocytes.
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