Human Calponin-1(CNN1)ELISA Kit

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Target Background

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Product Details

Description

This Human CNN1 ELISA Kit was designed for the quantitative measurement of Human CNN1 protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL.

Target Name

calponin 1, basic, smooth muscle

Alternative Names

Basic calponin ELISA Kit; Calponin 1 ELISA Kit; Calponin 1 basic smooth muscle ELISA Kit; Calponin H1 ELISA Kit; Calponin H1 smooth muscle ELISA Kit; Calponin-1 ELISA Kit; Calponin1 ELISA Kit; Calponins basic ELISA Kit; CNN 1 ELISA Kit; Cnn1 ELISA Kit; CNN1_HUMAN ELISA Kit; Epididymis secretory protein Li 14 ELISA Kit; HEL S 14 ELISA Kit; Sm Calp ELISA Kit; SMCC ELISA Kit; smooth muscle ELISA Kit

Abbreviation

CNN1

Uniprot No.

P51911

Species

Homo sapiens (Human)

Sample Types

serum, plasma

Detection Range

0.156 ng/mL-10 ng/mL

Sensitivity

0.039 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Cardiovascular

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of Human CNN1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	87
	Range %	84-92
1:2	Average %	95
	Range %	90-99
1:4	Average %	94
	Range %	85-99
1:8	Average %	104
	Range %	98-108

Recovery

The recovery of Human CNN1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	87-98
EDTA plasma (n=4)	87	83-94

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
10	2.255	2.345	2.300	2.202
5	1.715	1.756	1.736	1.638
2.5	1.064	1.024	1.044	0.946
1.25	0.523	0.515	0.519	0.421
0.625	0.334	0.324	0.329	0.231
0.312	0.251	0.234	0.243	0.145
0.156	0.162	0.159	0.161	0.063
0	0.097	0.098	0.098

ELISA Data Analysis

Watch ELISA data processing video & download Curve Expert if needed

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days

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Target Background

Function (From Uniprot)	Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity.
Gene References into Functions	Studied maternal serum calponin 1 levels in the prediction of delivery within 7 days among pregnancies complicated with threatened preterm labor. Of 73 women included in the study, found the maternal serum calponin 1 level was significantly high in women who delivered within 7 days vs. those who delivered after 7 days. PMID: 28068849 Loss of myoepithelial calponin was specifically associated with gain of the basal marker p63 in adjacent tumor cells. PMID: 26343330 Loss of CNN1 expression is associated with Ovarian Cancer. PMID: 26504022 CNN1 immunohistochemical staining allows ready recognition of intracytoplasmic inclusions, aiding diagnosis of infantile digital fibromatosis. PMID: 24117680 epsilonPKC/ERK/mTOR pathway activation induced by the rescued expression of CNN1 contributed to the improvement of cardiac dysfunction and pathological changes observed in transgenic mice. PMID: 24631115 Calponin knockdown inhibits autophosphorylation. PMID: 24350264 Expression of transforming growth factor-beta (4.3 +/- 1.4 vs 2.6 +/- 2.3, p = 0.01) and calponin (3.7 +/- 0.7 vs 0.6 +/- 1.1, p = 0.05) was lower from infants with vs without a PDA. PMID: 23731614 Calponin-1 expression in the uterine smooth muscle tissue of the body and the lower uterine segment of smooth muscle tissues was significantly different. PMID: 21051832 Inhibition of calponin-1 expression can inhibit uterine smooth muscle cell migration and cause the morphological change and rearrangement of F-actin without affecting its proliferation and apoptosis in vitro. PMID: 20584679 A single intronic CArG element is necessary but insufficient for proper CNN1 expression in vivo. CNN1 overexpression antagonizes arterial injury-induced neointimal formation. PMID: 21817093 D2-40 immunohistochemistry reliably identifies the myoepithelial cells of breast in a variety of lesions in a pattern similar to that of calponin and p63. PMID: 21326813 The point mutation S175T exhibited more resistance to actin rearrangement by cytochalasin D and PDBu than intact CNh1, suppressing cell motility induced by PDBu. PMID: 20530411 expression in tumor vessels is a new and useful means to predict prognosis of hepatocellular carcinoma after hepatic resection PMID: 11920541 results suggest that regulation of h1-calponin is involved in the regulation of differentiation of AR42J cells into insulin-producing cells PMID: 12606518 the calponin homology domain of Vav1 associates with calmodulin and is prerequisite to T cell antigen receptor-induced calcium release in Jurkat T lymphocytes PMID: 17550897 analysis of distinct sites of interaction that form the calponin: gelsolin complex and two calcium switches that control its activity PMID: 17556051 expression of vascular CNN1 in the peripheral region of colon cancer tissues was significantly reduced in association with tumor progression, lymphatic invasion, vascular invasion and recurrence. PMID: 17707120 Calponin and alpha-inhibin are the most useful positive markers for serous microcystic adenoma, and are negative in most entities in the differential diagnosis. PMID: 19391217 Show More Hide All
Protein Families	Calponin family
Tissue Specificity	Smooth muscle, and tissues containing significant amounts of smooth muscle.
Database Links	HGNC: 2155 OMIM: 600806 KEGG: hsa:1264 STRING: 9606.ENSP00000252456 UniGene: Hs.465929

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Check Code：

Code	CSB-E14109h
Size	96T,5×96T,10×96T
Trial Size
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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.