CNN1 Antibody

Code CSB-RA005655A0HU
Size US$350 How to order?
Image
  • Western Blot
    Positive WB detected in: 293 whole cell lysate, Mouse lung tissue
    All lanes: CNN1 antibody at 1.05μg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 34, 32 KDa
    Observed band size: 34 KDa

  • IHC image of CSB-RA005655A0HU diluted at 1:105 and staining in paraffin-embedded human heart tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

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Product Details

Description

The product CSB-RA005655A0HU is a recombinant CNN1 monoclonal antibody. It is generated by transfecting the human CNN1 gene-vector clones into the cell line for in vitro production and subsequent purification from the tissue culture supernatant (TCS). This CNN1 antibody can react with human CNN1 protein. It has undergone affinity-chromatography purification. And it is suitable for ELISA, WB, and IHC assays.

CNN1 is specifically expressed in differentiated mature smooth muscle cells and is implicated in the regulation and modulation of smooth muscle contraction. It is up-regulated in smooth muscle tissues during postnatal development with a higher content in the phasic smooth muscle of the digestive tract

Uniprot No. P51911
Target Names CNN1
Alternative Names Basic calponin antibody; Calponin 1 antibody; Calponin 1 basic smooth muscle antibody; Calponin H1 antibody; Calponin H1 smooth muscle antibody; Calponin-1 antibody; Calponin1 antibody; Calponins basic antibody; CNN 1 antibody; Cnn1 antibody; CNN1_HUMAN antibody; Epididymis secretory protein Li 14 antibody; HEL S 14 antibody; Sm Calp antibody; SMCC antibody; smooth muscle antibody
Species Reactivity Human, Mouse
Immunogen A synthesized peptide derived from human CNN1
Immunogen Species Homo sapiens (Human)
Conjugate Non-conjugated
Clonality Monoclonal
Isotype Rabbit IgG
Clone No. 2H1
Purification Method Affinity-chromatography
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form Liquid
Tested Applications ELISA, WB, IHC
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:5000
IHC 1:50-1:200
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function
Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity.
Gene References into Functions
  1. Studied maternal serum calponin 1 levels in the prediction of delivery within 7 days among pregnancies complicated with threatened preterm labor. Of 73 women included in the study, found the maternal serum calponin 1 level was significantly high in women who delivered within 7 days vs. those who delivered after 7 days. PMID: 28068849
  2. Loss of myoepithelial calponin was specifically associated with gain of the basal marker p63 in adjacent tumor cells. PMID: 26343330
  3. Loss of CNN1 expression is associated with Ovarian Cancer. PMID: 26504022
  4. CNN1 immunohistochemical staining allows ready recognition of intracytoplasmic inclusions, aiding diagnosis of infantile digital fibromatosis. PMID: 24117680
  5. epsilonPKC/ERK/mTOR pathway activation induced by the rescued expression of CNN1 contributed to the improvement of cardiac dysfunction and pathological changes observed in transgenic mice. PMID: 24631115
  6. Calponin knockdown inhibits autophosphorylation. PMID: 24350264
  7. Expression of transforming growth factor-beta (4.3 +/- 1.4 vs 2.6 +/- 2.3, p = 0.01) and calponin (3.7 +/- 0.7 vs 0.6 +/- 1.1, p = 0.05) was lower from infants with vs without a PDA. PMID: 23731614
  8. Calponin-1 expression in the uterine smooth muscle tissue of the body and the lower uterine segment of smooth muscle tissues was significantly different. PMID: 21051832
  9. Inhibition of calponin-1 expression can inhibit uterine smooth muscle cell migration and cause the morphological change and rearrangement of F-actin without affecting its proliferation and apoptosis in vitro. PMID: 20584679
  10. A single intronic CArG element is necessary but insufficient for proper CNN1 expression in vivo. CNN1 overexpression antagonizes arterial injury-induced neointimal formation. PMID: 21817093
  11. D2-40 immunohistochemistry reliably identifies the myoepithelial cells of breast in a variety of lesions in a pattern similar to that of calponin and p63. PMID: 21326813
  12. The point mutation S175T exhibited more resistance to actin rearrangement by cytochalasin D and PDBu than intact CNh1, suppressing cell motility induced by PDBu. PMID: 20530411
  13. expression in tumor vessels is a new and useful means to predict prognosis of hepatocellular carcinoma after hepatic resection PMID: 11920541
  14. results suggest that regulation of h1-calponin is involved in the regulation of differentiation of AR42J cells into insulin-producing cells PMID: 12606518
  15. the calponin homology domain of Vav1 associates with calmodulin and is prerequisite to T cell antigen receptor-induced calcium release in Jurkat T lymphocytes PMID: 17550897
  16. analysis of distinct sites of interaction that form the calponin: gelsolin complex and two calcium switches that control its activity PMID: 17556051
  17. expression of vascular CNN1 in the peripheral region of colon cancer tissues was significantly reduced in association with tumor progression, lymphatic invasion, vascular invasion and recurrence. PMID: 17707120
  18. Calponin and alpha-inhibin are the most useful positive markers for serous microcystic adenoma, and are negative in most entities in the differential diagnosis. PMID: 19391217

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Protein Families Calponin family
Tissue Specificity Smooth muscle, and tissues containing significant amounts of smooth muscle.
Database Links

HGNC: 2155

OMIM: 600806

KEGG: hsa:1264

STRING: 9606.ENSP00000252456

UniGene: Hs.465929

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