CNN1 Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA005655A0HU
Size	US$210
	Order now
Image	Western Blot Positive WB detected in: 293 whole cell lysate, Mouse lung tissue All lanes: CNN1 antibody at 1.05μg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 34, 32 KDa Observed band size: 34 KDa IHC image of CSB-RA005655A0HU diluted at 1:105 and staining in paraffin-embedded human heart tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Have Questions?	Leave a Message or Start an on-line Chat

Product Details
Related Products
Customer Reviews and Q&A
Target Background

Product Details

Uniprot No.

P51911

Target Names

CNN1

Alternative Names

Basic calponin antibody; Calponin 1 antibody; Calponin 1 basic smooth muscle antibody; Calponin H1 antibody; Calponin H1 smooth muscle antibody; Calponin-1 antibody; Calponin1 antibody; Calponins basic antibody; CNN 1 antibody; Cnn1 antibody; CNN1_HUMAN antibody; Epididymis secretory protein Li 14 antibody; HEL S 14 antibody; Sm Calp antibody; SMCC antibody; smooth muscle antibody

Species Reactivity

Human, Mouse

Immunogen

A synthesized peptide derived from human CNN1

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

2H1

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

Calponin-1 serves as a fundamental regulator of smooth muscle contraction, functioning as an actin-binding protein that modulates actomyosin interactions and cytoskeletal dynamics. As a definitive marker of smooth muscle differentiation, CNN1 plays essential roles in vascular biology, cardiac development, and tissue remodeling research. Its expression patterns provide valuable insights into smooth muscle phenotype switching, a process implicated in cardiovascular diseases and fibrotic conditions.

This recombinant monoclonal antibody, generated from clone 2H1, offers the reproducibility and consistency that demanding research protocols require. Because the antibody sequence is defined and production occurs in controlled recombinant systems, you can expect uniform performance across experiments and over time, eliminating the lot-to-lot variability that can compromise longitudinal studies or multi-site collaborations. The rabbit IgG format, raised against a synthetic peptide derived from human CNN1, provides high specificity for your target.

Validation studies confirm reliable performance across multiple experimental platforms. In western blot applications, the antibody detects CNN1 at the expected 34 kDa molecular weight in both 293 whole cell lysate and mouse lung tissue, demonstrating cross-species reactivity between human and mouse samples. Recommended dilutions range from 1:500 to 1:5000, allowing you to optimize signal intensity for your specific sample types. For tissue localization studies, immunohistochemistry validation in paraffin-embedded human heart tissue shows clear staining using standard antigen retrieval protocols, with effective dilutions between 1:50 and 1:200.

Whether investigating smooth muscle differentiation, cardiovascular signal transduction pathways, or cytoskeletal regulation, this antibody provides a dependable tool for advancing your research objectives.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

■ Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

Function

Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity.

Gene References into Functions

Studied maternal serum calponin 1 levels in the prediction of delivery within 7 days among pregnancies complicated with threatened preterm labor. Of 73 women included in the study, found the maternal serum calponin 1 level was significantly high in women who delivered within 7 days vs. those who delivered after 7 days. PMID: 28068849
Loss of myoepithelial calponin was specifically associated with gain of the basal marker p63 in adjacent tumor cells. PMID: 26343330
Loss of CNN1 expression is associated with Ovarian Cancer. PMID: 26504022
CNN1 immunohistochemical staining allows ready recognition of intracytoplasmic inclusions, aiding diagnosis of infantile digital fibromatosis. PMID: 24117680
epsilonPKC/ERK/mTOR pathway activation induced by the rescued expression of CNN1 contributed to the improvement of cardiac dysfunction and pathological changes observed in transgenic mice. PMID: 24631115
Calponin knockdown inhibits autophosphorylation. PMID: 24350264
Expression of transforming growth factor-beta (4.3 +/- 1.4 vs 2.6 +/- 2.3, p = 0.01) and calponin (3.7 +/- 0.7 vs 0.6 +/- 1.1, p = 0.05) was lower from infants with vs without a PDA. PMID: 23731614
Calponin-1 expression in the uterine smooth muscle tissue of the body and the lower uterine segment of smooth muscle tissues was significantly different. PMID: 21051832
Inhibition of calponin-1 expression can inhibit uterine smooth muscle cell migration and cause the morphological change and rearrangement of F-actin without affecting its proliferation and apoptosis in vitro. PMID: 20584679
A single intronic CArG element is necessary but insufficient for proper CNN1 expression in vivo. CNN1 overexpression antagonizes arterial injury-induced neointimal formation. PMID: 21817093
D2-40 immunohistochemistry reliably identifies the myoepithelial cells of breast in a variety of lesions in a pattern similar to that of calponin and p63. PMID: 21326813
The point mutation S175T exhibited more resistance to actin rearrangement by cytochalasin D and PDBu than intact CNh1, suppressing cell motility induced by PDBu. PMID: 20530411
expression in tumor vessels is a new and useful means to predict prognosis of hepatocellular carcinoma after hepatic resection PMID: 11920541
results suggest that regulation of h1-calponin is involved in the regulation of differentiation of AR42J cells into insulin-producing cells PMID: 12606518
the calponin homology domain of Vav1 associates with calmodulin and is prerequisite to T cell antigen receptor-induced calcium release in Jurkat T lymphocytes PMID: 17550897
analysis of distinct sites of interaction that form the calponin: gelsolin complex and two calcium switches that control its activity PMID: 17556051
expression of vascular CNN1 in the peripheral region of colon cancer tissues was significantly reduced in association with tumor progression, lymphatic invasion, vascular invasion and recurrence. PMID: 17707120
Calponin and alpha-inhibin are the most useful positive markers for serous microcystic adenoma, and are negative in most entities in the differential diagnosis. PMID: 19391217

Hide All

Protein Families

Calponin family

Tissue Specificity

Smooth muscle, and tissues containing significant amounts of smooth muscle.

Database Links

HGNC: 2155

OMIM: 600806

KEGG: hsa:1264

STRING: 9606.ENSP00000252456

UniGene: Hs.465929

Promotions

Email: [email protected]
Distributors Worldwide

Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

MYL9: a New Member of Myosin Motors, a Key Driver of Cancer Cell Dynamic Regulation? Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

Join us with

Subscribe newsletter

Leave a message

Message

Product >> Product Name：
Product >> Code：
Name： *
Email： *
Company/Organization：
Phone：
Since emails may get flagged by spam filters occasionally, we recommend leaving your phone number as a precaution. Your information is safe with us.
Question/Comment： *
Your Region： *
I'd like to receive email newsletters from Cusabio

Yes

No
How do you know CUSABIO?

Email

Distributor

Search engine

Advertisement

Others
CAPTCHA verification：
Please enter the characters shown. Click the image to refresh if unclear.

Place an order now

I. Product details

Product name *

Code *

Size *

Quantity *

Message

II. Contact details

Name *

Email address *

Company/Organization

Phone

III. Ship To

Address *

City *

State/Province *

Zip/Post code *

Country *

Attn Name *

Phone# *

IV. Bill To Same as Shipping Address