Human Cartilage acidic protein 1(CRTAC1) ELISA kit

Product Details

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Target Background

Product Details

Target Name

cartilage acidic protein 1

Alternative Names

68 kDa chondrocyte expressed protein ELISA Kit; 68 kDa chondrocyte-expressed protein ELISA Kit; Acidic secreted protein in cartilage ELISA Kit; ASPIC ELISA Kit; ASPIC1 ELISA Kit; Cartilage acidic protein 1 ELISA Kit; Cartilage acidic protein 1 precursor ELISA Kit; CEP 68 ELISA Kit; CEP-68 ELISA Kit; CEP68 ELISA Kit; Chondrocyte expressed protein 68 kDa CEP 68 ELISA Kit; CRAC1_HUMAN ELISA Kit; CRTAC1 ELISA Kit; Protein CRTAC1-B ELISA Kit; W307 ELISA Kit

Abbreviation

CRTAC1

Uniprot No.

Q9NQ79

Species

Homo sapiens (Human)

Sample Types

serum, plasma, tissue homogenates

Detection Range

4.68 ng/mL-300 ng/mL

Sensitivity

1.17 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Signal Transduction

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human CRTAC1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:5	Average %	91
	Range %	87-96
1:10	Average %	97
	Range %	93-101
1:20	Average %	102
	Range %	99-103
1:40	Average %	88
	Range %	86-91

Recovery

The recovery of human CRTAC1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	86-93
EDTA plasma (n=4)	102	98-107

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
300	2.522	2.735	2.629	2.444
150	2.043	2.102	2.073	1.888
75	1.317	1.315	1.316	1.131
37.5	0.875	0.857	0.866	0.681
18.75	0.470	0.445	0.458	0.273
9.37	0.366	0.380	0.373	0.188
4.68	0.254	0.252	0.253	0.068
0	0.186	0.184	0.185

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

Description

This Human CRTAC1 ELISA Kit was designed for the quantitative measurement of Human CRTAC1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 4.68 ng/mL-300 ng/mL and the sensitivity is 1.17 ng/mL.

Target Background

Gene References into Functions

Confirm the increase of CRTAC1 in cartilage biopsies from OA patients undergoing joint replacement by real-time PCR and immunohistochemistry. Furthermore, we report that proinflammatory cytokines interleukin-1beta and tumor necrosis factor alpha upregulate CRTAC1 expression in primary articular chondrocytes and synovial fibroblasts. PMID: 27415616
It was shown for the first time the propensity of CRTAC1 to form amyloid-like structures, and it was hypothesized that the aggregating property of CRTAC1 may be related to its disease-association. PMID: 27862299
These results provided new insights into the mechanism of cataract development, and demonstrated that CRTAC1 could be a potentially novel target for cataract treatment. PMID: 27855397
CRTAC1 acquired an alternate last exon from the tail-to-tail oriented neighbouring gene in humans resulting in the glycosylated isoform CRTAC1-A which represents a new extracellular matrix molecule of articular cartilage. PMID: 17074475
in bone fracture patients, 12 proteins were related to bone/cartilage metabolism, including: TGF-beta induced protein IG-H(3), cartilage acidic protein 1, procollagen C proteinase enhancer protein and TGF-beta receptor III. PMID: 17602227

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Subcellular Location

Secreted, extracellular space, extracellular matrix.

Tissue Specificity

Expressed in the interterritorial matrix of articular deep zone cartilage (at protein level). Isoform 1 and isoform 2 are expressed in brain. Isoform 1 is detected in lung and chondrocytes. Detected in cartilage, bone, cultured chondrocytes and lung, and

Database Links

HGNC: 14882

OMIM: 606276

KEGG: hsa:55118

STRING: 9606.ENSP00000359629

UniGene: Hs.500736

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Code	CSB-EL005997HU
Size	96T,5×96T,10×96T
Price	Request a Quote or Start an on-line Chat
Trial Size	24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.