Human Claudin-3(CLDN3) ELISA kit

Code CSB-EL005505HU
Size 96T,5×96T,10×96T How to order?
Trial Size 24T ELISA kits trial application
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Product Details

Description

This Human CLDN3 ELISA Kit was designed for the quantitative measurement of Human CLDN3 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 25 pg/mL-1600 pg/mL and the sensitivity is 6.25 pg/mL.

Target Name claudin 3
Alternative Names CLDN3; C7orf1; CPETR2; Claudin-3; Clostridium perfringens enterotoxin receptor 2; CPE-R 2; CPE-receptor 2; Rat ventral prostate.1 protein homolog; hRVP1
Abbreviation CLDN3
Uniprot No. O15551
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 25 pg/mL-1600 pg/mL
Sensitivity 6.25 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cancer
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human CLDN3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 89  
Range % 86-93  
1:2 Average % 89  
Range % 85-92  
1:4 Average % 94  
Range % 89-98  
1:8 Average % 102  
Range % 98-105  
Recovery
The recovery of human CLDN3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 96 93-102  
EDTA plasma (n=4) 87 80-93  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
1600 2.116 2.294 2.205 2.009  
800 1.635 1.532 1.584 1.388  
400 1.144 1.124 1.134 0.938  
200 0.773 0.750 0.762 0.566  
100 0.542 0.537 0.540 0.344  
50 0.423 0.439 0.431 0.235  
25 0.302 0.309 0.306 0.110  
0 0.195 0.196 0.196    
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Function
(From Uniprot)
Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity.
Gene References into Functions
  1. Claudin3 promoter methylation status (HR: 5.67; 95% CI: 2.2714.17) but not claudin3 expression was an independent predictor of survival. Claudin3 promoter hypermethylation reduces claudin3 expression and independently predicts poor prognosis. PMID: 29749528
  2. Immunohistochemical expression levels of cytoplasmic claudins 3 and 7 appear to be novel prognostic factors in triple-negative breast cancer. PMID: 29482498
  3. CLDN3 could be further evaluated as a novel biomarker for predicting the prognosis of lung squamous cell carcinoma (SqCC) and as a target for the treatment of lung SqCC in the future. PMID: 29511369
  4. We describe a novel workflow, completely covering the analysis of CLDN3 as an exemplary exosome-based biomarker for prostate cancer from in vitro profiling of cancer exosomes over in silico identification and in vitro retesting to clinical validation. PMID: 28396511
  5. Data indicate that CDH11, ICAM1 and CLDN3 were overexpressed in tumors when compared to normal esophagus, normal gastric and non-dysplastic Barrett's. PMID: 27363029
  6. Study provide first biochemically and clinically validated evidence to support a colorectal cancer-suppressive function of claudin-3 by serving as a conjoint rheostat for regulating Stat-3 and Wnt/beta-catenin-signaling. PMID: 28783170
  7. These tumor samples express CD44 protein at low rather than high levels. There is no correlation between CLDN3 gene expression and protein expression in these CPTAC samples; hence, the claudin-low subtype defined by gene expression is not the same group of tumors as that defined by low expression of CLDN3 protein. PMID: 28287265
  8. Increased expression of intestinal epithelial claudin-1 with downregulation of claudin-3 has been observed in intestinal inflammatory disorders. PMID: 28493289
  9. Data show that the charge of Lys65 in claudin 1 (Cldn1) and Glu158 in claudin 3 (Cldn3), and of Gln57 in claudin 5 (Cldn5) are necessary for tight junction (TJ) strand formation. PMID: 28415153
  10. Mislocalization claudin-3 to nucleus in colon cancer and mislocalization claudin-4 to nucleus in adenomas of the colon were detected for the first time. . PMID: 28295005
  11. Intracellular zinc has an essential role in the maintenance of the intestinal epithelial tight junction barrier through regulation of occludin proteolysis and claudin-3 transcription. PMID: 27151944
  12. permeability barriers and affected cell morphology, proliferation, migration, AKT signaling, and gene expression. When claudins are exogenously expressed, ARPE-19 more closely model native RPE. PMID: 27593915
  13. localization of Cldn3, Cldn7 and Cldn10 proteins in the different compartments of murine endometrium up to day 8.5 of pregnancy (dpc) as well as in human endometrium and first trimester decidua PMID: 26340953
  14. Cln-3 plays a vital role in TNF-modulated paracellular permeability in submandibular epithelium. PMID: 26148935
  15. Further in vitro studies suggested that the isolated MAbs possessed the desired binding properties for the detection or targeting of CLDN3. PMID: 25744656
  16. that Claudin-3 expression was restricted to the apical pole of ependymocytes in the subcommissural organ PMID: 24974365
  17. The expressions of MARVELD2, CLDN1 and CLDN3 mRNA were significantly lower in cholesteatoma tissue and may be involved in epithelium permeability. PMID: 25319490
  18. Univariate analyses indicated that the T stage, lymph node metastasis, the TNM stage, and the expression of claudin-3, beta-catenin, and vimentin were significant predictors for overall survival (OS). PMID: 25820701
  19. Data from live-cell imaging suggest at least two different cis-interaction interfaces within CLDN3 homopolymers as well as within CLDN1/CLDN3 heteropolymers. PMID: 25849148
  20. our findings demonstrated that CLDN3 is an epigenetically silenced metastasis suppressor gene in Hepatocellular carcinoma PMID: 25277196
  21. Study highlights a profound role for the choroid plexus in the pathogenesis of multiple sclerosis, and implies that CLDN3 may be regarded as a crucial and novel determinant of blood-cerebrospinal fluid barrier integrity PMID: 24356983
  22. Claudin 3 was expressed in all non-goblet columnar lined oesophagus, Barrett's oesophagus, high grade dysplasia and adenocarcinoma. PMID: 24290871
  23. Claudin-3 overexpression increases the malignant potential of colorectal cancer cells. PMID: 24069372
  24. folding and assembly of CLDN3 and CLDN5 into the tight junction are controlled by non-conserved residues in the transmembrane 3 and extracellular loop 2 segments PMID: 24478310
  25. Our comparative analysis of CLDN3 profile in breast and ovarian cancer clearly indicates organ specificity. PMID: 23529315
  26. Snail and Claudin-3 may play important roles in invasion and metastasis in NSCLC PMID: 23075682
  27. Claudin-3 expression in uterine luminal epithelium is stimulated by progesterone and suppressed by heparin-binding epidermal growth factor-like growth factor. PMID: 23909989
  28. High CLDN3 expression is associated with tumor growth and metastases. PMID: 23097631
  29. Dow-regulation of Claudin-3 is associated with the progression of early gastric adenocarcinomas. PMID: 22290341
  30. CLDN3 may have a role in ovarian cancer, and its inhibition by short hairpin RNA could be a treatment strategy. PMID: 21519794
  31. Analysis of staining intensities of CLDN 1 and 3 is useful as an auxiliary diagnostic and prognostic tool in patients with salivary gland mucoepidermoid carcinoma. PMID: 21184237
  32. demonstrate that claudin-3 alters the tight junction meshwork and seals the paracellular pathway against the passage of small ions of either charge and uncharged solutes PMID: 20655293
  33. Claudin-3 expression in Epstein-Barr virus-associated nasopharyngeal carcinoma was variable PMID: 20204275
  34. Increased expressions of CLDN 2 and 3 suggest structural changes of tight junction in coeliac disease which may be, at least in part, responsible for increased permeability and proliferation observed in coeliac disease. PMID: 20143085
  35. Here we show for the first time in both an experimental and clinical setting a strong relation between intestinal tight junction loss and urinary claudin-3 levels PMID: 19525861
  36. Airway tight junctions are regulated by claudin interactions that confer the selectivity of the junction. PMID: 12909588
  37. up-regulation of DDR1, CLDN3, and epithelial cell adhesion molecule are early events in the development of epithelial ovarian cancer PMID: 15240533
  38. in breast tissue, CLDN3 expression is similar in tumours and surrounding normal tissue, as demonstrated by immunohistochemistry and real-time PCR PMID: 15743508
  39. claudin-3 phosphorylation by PKA may provide a mechanism for the disruption of tight junctions in ovarian cancer PMID: 15905176
  40. The gene expression profile of hepatic stem cells throughout life consists of high levels of expression of claudin-3 (CLDN-3). PMID: 16627685
  41. Claudin tight junction proteins in endoscopy biopsy samples showed Barrett's metaplasia contains more claudin-2 and claudin-3 than found in normal esophageal mucosa, but markedly lower claudins 1 and 5, indicating very different tight junction barriers. PMID: 17103306
  42. Overexpression of claudin-3 is associated with uterine serous papillary carcinoma PMID: 17326053
  43. When compared, small-cell-lung cancers, carcinoid tumors, and adenocarcinomas revealed significant differences re: CLDN3 expression. PMID: 17418912
  44. CLDN3 overexpression can be used as a prognostic indicator in ovarian serous carcinomas and it may be a promising target for antibody-based therapy of ovarian carcinomas. PMID: 17647191
  45. siRNA-mediated knockdown of Sp1 led to a significant decrease of CLDN3 expression at both the mRNA and protein levels, demonstrating a crucial role for this transcription factor in the regulation of CLDN3. PMID: 17986852
  46. Claudin-3 and claudin-7 expression in effusions independently predicts poor survival in ovarian cancer. PMID: 18439941
  47. claudins 1 and 3 had a significant effect on overall survival in patients with urothelial carcinoma of the upper urinary tract. PMID: 18550469
  48. This is the first study to demonstrate that claudin-3 is involved in the barrier function of gastric epithelial cells and that rebamipide abolishes the H2O2-induced decrease in claudin-3 protein. PMID: 18774778
  49. For the first time this study proves the presence of Claudin-1, Claudin-3 and Claudin-5 in ECV304 (obtained from ECACC) cell layers and the inducibility of their expression by glioma-conditioned media. PMID: 18817843
  50. Using a panel of four genes (AHRR, p16INK4a, MT1G, and CLDN3) resulted in sensitivity and specificity of 50% and 68%, respectively and may have utility for early detection of esophageal squamous dysplasia and early ESCC. PMID: 19137073

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Involvement in disease CLDN3 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region.
Subcellular Location Cell junction, tight junction. Cell membrane; Multi-pass membrane protein.
Protein Families Claudin family
Database Links

HGNC: 2045

OMIM: 602910

KEGG: hsa:1365

STRING: 9606.ENSP00000378577

UniGene: Hs.647023

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