Recombinant Human Claudin-3 (CLDN3)-VLPs (Active)

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Code CSB-MP005505HU
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Size $630
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  • CSB-MP005505HU is detected by Mouse anti-6*His monoclonal antibody.
  • Activity
    Measured by its binding ability in a functional ELISA. Immobilized Human CLDN3 at 10μg/mL can bind Anti-CLDN3 recombinant antibody (CSB-RA005505MA1HU), the EC50 is 23.62-34.37 ng/mL.VLPs (CSB-MP3838) is negative control. Biological Activity Assay
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Product Details

Endotoxin
Less than 1.0 EU/ug as determined by LAL method.
Activity
Measured by its binding ability in a functional ELISA. Immobilized Human CLDN3 at 10 μg/mL can bind Anti-CLDN3 recombinant antibody (CSB-RA005505MA1HU), the EC50 is 23.62-34.37 ng/mL.VLPs (CSB-MP3838) is negative control.
Target Names
Uniprot No.
Alternative Names
(Clostridium perfringens enterotoxin receptor 2)(CPE-R 2; CPE-receptor 2)(Rat ventral prostate.1 protein homolog)(hRVP1)
Molecular Characterization
Species
Homo sapiens (Human)
Source
Mammalian cell
Expression Region
1-220aa
Target Protein Sequence
MSMGLEITGTALAVLGWLGTIVCCALPMWRVSAFIGSNIITSQNIWEGLWMNCVVQSTGQMQCKVYDSLLALPQDLQAARALIVVAILLAAFGLLVALVGAQCTNCVQDDTAKAKITIVAGVLFLLAALLTLVPVSWSANTIIRDFYNPVVPEAQKREMGAGLYVGWAAAALQLLGGALLCCSCPPREKKYTATKVVYSAPRSTGPGASLGTGYDRKDYV
Mol. Weight
24.7 kDa
Protein Length
Full Length
Tag Info
C-terminal 10xHis-tagged (This tag can be tested only under denaturing conditions)
Form
Lyophilized powder
Note: We will default ship it in lyophilized form with normal bule ice packs. However, if you request to ship in liquid form, it needs to be shipped with dry ice, please communicate with us in advance and extra fees for dry ice and dry ice box will be charged.
Buffer
Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description

This Human CLDN3 recombinant protein was produced in mammalian cell, where the gene sequence encoding Human CLDN3 (1-220aa) was expressed with the C-terminal 10xHis-tag. The activity was validated.
In addition, this recombinant Human CLDN3 protein was developed through the Virus-Like Particles (VLPs) Platform. It is a 4-pass transmembrane protein.

Claudin-3 (CLDN3) is a member of the Claudin tight junction protein family and an important protein molecule that forms the tight junction between cells. It plays an important role in forming the blood-brain physical barrier of tight junctions and maintaining the stability of the cellular environment. Recent studies have found that signaling molecules such as zinc finger transcription factor and epidermal growth factor are involved in regulating the gene expression of CLDN3, and the abnormal expression of CLDN3 protein is closely related to the occurrence and development of various malignant tumors.
CLDN3 has three interrelated biological properties: barrier function, palisade function and signal transduction function. Tight junctions consist of a branched network of closed cables, each acting independently of the others. The ability of tight junctions to prevent the passage of ions increases exponentially with the number of closing cords. Each occlusal cord consists of a row of transmembrane proteins embedded in two plasma membranes, and the extracellular domains of the proteins make them directly associated with each other.

Customer Reviews and Q&A

 Customer Reviews
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Applications : Enzymatic activity in vitro

Review: Claudin-3 (CLDN3) is a member of the Claudin family of tight junction proteins and plays an important role in forming intercellular tight junctions. The abnormal expression of the CLDN3 protein is closely related to the occurrence and development of various malignant tumors, making it one of the hot targets in current research. The Claudin-3 is a four times transmembrane protein. The full-length recombinant human CLDN3 protein expressed by CUSABIO through its VLP platform has good activity. I used the CSB-MP005505HU product for an ELISA experiment, and the EC50 was measured to be 3.157-5.258 ng/ml, which can be used for my subsequent experimental research, and the results have been promising. The protein is stable with good repeatability, so I will continue to purchase in the future.

By Anonymous

 Q&A
Q:

Could you kindly tell me the purification method of CSB-MP005505HU (Recombinant Human Claudin-3(CLDN3)-VLPs (Active)).
If a virus was used or not and what kind?

A:

The purification method we usually use is Ultracentrifugation + affinity purification.
VLPs (Virus Like Particles) are nanoscale particles formed by the automatic assembly of one or more capsid proteins of enveloped viruses. VLP particles do not contain viral nucleic acid, cannot replicate autonomously, are highly safe, and have an overall structure similar to virus particles.

Target Background

Function
Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity.
Gene References into Functions
  1. Claudin3 promoter methylation status (HR: 5.67; 95% CI: 2.2714.17) but not claudin3 expression was an independent predictor of survival. Claudin3 promoter hypermethylation reduces claudin3 expression and independently predicts poor prognosis. PMID: 29749528
  2. Immunohistochemical expression levels of cytoplasmic claudins 3 and 7 appear to be novel prognostic factors in triple-negative breast cancer. PMID: 29482498
  3. CLDN3 could be further evaluated as a novel biomarker for predicting the prognosis of lung squamous cell carcinoma (SqCC) and as a target for the treatment of lung SqCC in the future. PMID: 29511369
  4. We describe a novel workflow, completely covering the analysis of CLDN3 as an exemplary exosome-based biomarker for prostate cancer from in vitro profiling of cancer exosomes over in silico identification and in vitro retesting to clinical validation. PMID: 28396511
  5. Data indicate that CDH11, ICAM1 and CLDN3 were overexpressed in tumors when compared to normal esophagus, normal gastric and non-dysplastic Barrett's. PMID: 27363029
  6. Study provide first biochemically and clinically validated evidence to support a colorectal cancer-suppressive function of claudin-3 by serving as a conjoint rheostat for regulating Stat-3 and Wnt/beta-catenin-signaling. PMID: 28783170
  7. These tumor samples express CD44 protein at low rather than high levels. There is no correlation between CLDN3 gene expression and protein expression in these CPTAC samples; hence, the claudin-low subtype defined by gene expression is not the same group of tumors as that defined by low expression of CLDN3 protein. PMID: 28287265
  8. Increased expression of intestinal epithelial claudin-1 with downregulation of claudin-3 has been observed in intestinal inflammatory disorders. PMID: 28493289
  9. Data show that the charge of Lys65 in claudin 1 (Cldn1) and Glu158 in claudin 3 (Cldn3), and of Gln57 in claudin 5 (Cldn5) are necessary for tight junction (TJ) strand formation. PMID: 28415153
  10. Mislocalization claudin-3 to nucleus in colon cancer and mislocalization claudin-4 to nucleus in adenomas of the colon were detected for the first time. . PMID: 28295005
  11. Intracellular zinc has an essential role in the maintenance of the intestinal epithelial tight junction barrier through regulation of occludin proteolysis and claudin-3 transcription. PMID: 27151944
  12. permeability barriers and affected cell morphology, proliferation, migration, AKT signaling, and gene expression. When claudins are exogenously expressed, ARPE-19 more closely model native RPE. PMID: 27593915
  13. localization of Cldn3, Cldn7 and Cldn10 proteins in the different compartments of murine endometrium up to day 8.5 of pregnancy (dpc) as well as in human endometrium and first trimester decidua PMID: 26340953
  14. Cln-3 plays a vital role in TNF-modulated paracellular permeability in submandibular epithelium. PMID: 26148935
  15. Further in vitro studies suggested that the isolated MAbs possessed the desired binding properties for the detection or targeting of CLDN3. PMID: 25744656
  16. that Claudin-3 expression was restricted to the apical pole of ependymocytes in the subcommissural organ PMID: 24974365
  17. The expressions of MARVELD2, CLDN1 and CLDN3 mRNA were significantly lower in cholesteatoma tissue and may be involved in epithelium permeability. PMID: 25319490
  18. Univariate analyses indicated that the T stage, lymph node metastasis, the TNM stage, and the expression of claudin-3, beta-catenin, and vimentin were significant predictors for overall survival (OS). PMID: 25820701
  19. Data from live-cell imaging suggest at least two different cis-interaction interfaces within CLDN3 homopolymers as well as within CLDN1/CLDN3 heteropolymers. PMID: 25849148
  20. our findings demonstrated that CLDN3 is an epigenetically silenced metastasis suppressor gene in Hepatocellular carcinoma PMID: 25277196
  21. Study highlights a profound role for the choroid plexus in the pathogenesis of multiple sclerosis, and implies that CLDN3 may be regarded as a crucial and novel determinant of blood-cerebrospinal fluid barrier integrity PMID: 24356983
  22. Claudin 3 was expressed in all non-goblet columnar lined oesophagus, Barrett's oesophagus, high grade dysplasia and adenocarcinoma. PMID: 24290871
  23. Claudin-3 overexpression increases the malignant potential of colorectal cancer cells. PMID: 24069372
  24. folding and assembly of CLDN3 and CLDN5 into the tight junction are controlled by non-conserved residues in the transmembrane 3 and extracellular loop 2 segments PMID: 24478310
  25. Our comparative analysis of CLDN3 profile in breast and ovarian cancer clearly indicates organ specificity. PMID: 23529315
  26. Snail and Claudin-3 may play important roles in invasion and metastasis in NSCLC PMID: 23075682
  27. Claudin-3 expression in uterine luminal epithelium is stimulated by progesterone and suppressed by heparin-binding epidermal growth factor-like growth factor. PMID: 23909989
  28. High CLDN3 expression is associated with tumor growth and metastases. PMID: 23097631
  29. Dow-regulation of Claudin-3 is associated with the progression of early gastric adenocarcinomas. PMID: 22290341
  30. CLDN3 may have a role in ovarian cancer, and its inhibition by short hairpin RNA could be a treatment strategy. PMID: 21519794
  31. Analysis of staining intensities of CLDN 1 and 3 is useful as an auxiliary diagnostic and prognostic tool in patients with salivary gland mucoepidermoid carcinoma. PMID: 21184237
  32. demonstrate that claudin-3 alters the tight junction meshwork and seals the paracellular pathway against the passage of small ions of either charge and uncharged solutes PMID: 20655293
  33. Claudin-3 expression in Epstein-Barr virus-associated nasopharyngeal carcinoma was variable PMID: 20204275
  34. Increased expressions of CLDN 2 and 3 suggest structural changes of tight junction in coeliac disease which may be, at least in part, responsible for increased permeability and proliferation observed in coeliac disease. PMID: 20143085
  35. Here we show for the first time in both an experimental and clinical setting a strong relation between intestinal tight junction loss and urinary claudin-3 levels PMID: 19525861
  36. Airway tight junctions are regulated by claudin interactions that confer the selectivity of the junction. PMID: 12909588
  37. up-regulation of DDR1, CLDN3, and epithelial cell adhesion molecule are early events in the development of epithelial ovarian cancer PMID: 15240533
  38. in breast tissue, CLDN3 expression is similar in tumours and surrounding normal tissue, as demonstrated by immunohistochemistry and real-time PCR PMID: 15743508
  39. claudin-3 phosphorylation by PKA may provide a mechanism for the disruption of tight junctions in ovarian cancer PMID: 15905176
  40. The gene expression profile of hepatic stem cells throughout life consists of high levels of expression of claudin-3 (CLDN-3). PMID: 16627685
  41. Claudin tight junction proteins in endoscopy biopsy samples showed Barrett's metaplasia contains more claudin-2 and claudin-3 than found in normal esophageal mucosa, but markedly lower claudins 1 and 5, indicating very different tight junction barriers. PMID: 17103306
  42. Overexpression of claudin-3 is associated with uterine serous papillary carcinoma PMID: 17326053
  43. When compared, small-cell-lung cancers, carcinoid tumors, and adenocarcinomas revealed significant differences re: CLDN3 expression. PMID: 17418912
  44. CLDN3 overexpression can be used as a prognostic indicator in ovarian serous carcinomas and it may be a promising target for antibody-based therapy of ovarian carcinomas. PMID: 17647191
  45. siRNA-mediated knockdown of Sp1 led to a significant decrease of CLDN3 expression at both the mRNA and protein levels, demonstrating a crucial role for this transcription factor in the regulation of CLDN3. PMID: 17986852
  46. Claudin-3 and claudin-7 expression in effusions independently predicts poor survival in ovarian cancer. PMID: 18439941
  47. claudins 1 and 3 had a significant effect on overall survival in patients with urothelial carcinoma of the upper urinary tract. PMID: 18550469
  48. This is the first study to demonstrate that claudin-3 is involved in the barrier function of gastric epithelial cells and that rebamipide abolishes the H2O2-induced decrease in claudin-3 protein. PMID: 18774778
  49. For the first time this study proves the presence of Claudin-1, Claudin-3 and Claudin-5 in ECV304 (obtained from ECACC) cell layers and the inducibility of their expression by glioma-conditioned media. PMID: 18817843
  50. Using a panel of four genes (AHRR, p16INK4a, MT1G, and CLDN3) resulted in sensitivity and specificity of 50% and 68%, respectively and may have utility for early detection of esophageal squamous dysplasia and early ESCC. PMID: 19137073

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Involvement in disease
CLDN3 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region.
Subcellular Location
Cell junction, tight junction. Cell membrane; Multi-pass membrane protein.
Protein Families
Claudin family
Database Links

HGNC: 2045

OMIM: 602910

KEGG: hsa:1365

STRING: 9606.ENSP00000378577

UniGene: Hs.647023

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