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| Code | CSB-MP005505HU |
| Size | $6678 |
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| Description |
This Human CLDN3 recombinant protein was produced in mammalian cell, where the gene sequence encoding Human CLDN3 (1-220aa) was expressed with the C-terminal 10xHis-tag. The activity was validated. Claudin-3 (CLDN3) is a member of the Claudin tight junction protein family and an important protein molecule that forms the tight junction between cells. It plays an important role in forming the blood-brain physical barrier of tight junctions and maintaining the stability of the cellular environment. Recent studies have found that signaling molecules such as zinc finger transcription factor and epidermal growth factor are involved in regulating the gene expression of CLDN3, and the abnormal expression of CLDN3 protein is closely related to the occurrence and development of various malignant tumors. |
| Endotoxin | Less than 1.0 EU/ug as determined by LAL method. |
| Activity | Measured by its binding ability in a functional ELISA. Immobilized Human CLDN3 at 10 μg/mL can bind Anti-CLDN3 recombinant antibody (CSB-RA005505MA1HU)， the EC50 is 23.62-34.37 ng/mL.VLPs (CSB-MP3838) is negative control. |
| Target Names | CLDN3 |
| Uniprot No. | O15551 |
| Alternative Names |
(Clostridium perfringens enterotoxin receptor 2)(CPE-R 2; CPE-receptor 2)(Rat ventral prostate.1 protein homolog)(hRVP1)
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| Molecular Characterization |  |
| Species | Homo sapiens (Human) |
| Source | Mammalian cell |
| Expression Region | 1-220aa |
| Target Protein Sequence | MSMGLEITGTALAVLGWLGTIVCCALPMWRVSAFIGSNIITSQNIWEGLWMNCVVQSTGQMQCKVYDSLLALPQDLQAARALIVVAILLAAFGLLVALVGAQCTNCVQDDTAKAKITIVAGVLFLLAALLTLVPVSWSANTIIRDFYNPVVPEAQKREMGAGLYVGWAAAALQLLGGALLCCSCPPREKKYTATKVVYSAPRSTGPGASLGTGYDRKDYV |
| Mol. Weight | 24.7 kDa |
| Protein Length | Full Length |
| Tag Info |
C-terminal 10xHis-tagged |
| Form |
Lyophilized powder Note: We will default ship it in lyophilized form with normal bule ice packs. However, if you request to ship in liquid form, it needs to be shipped with dry ice, please communicate with us in advance and extra fees for dry ice and dry ice box will be charged. |
| Buffer | Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4 |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference. |
| Troubleshooting and FAQs |
Protein FAQs |
| Storage Condition | Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles. |
| Shelf Life | The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. |
| Lead Time | 3-7 business days |
| Notes | Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week. |
| Datasheet & COA | Please contact us to get it. |
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| Function |
Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity.
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| Gene References into Functions |
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| Involvement in disease | CLDN3 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region. |
| Subcellular Location | Cell junction, tight junction. Cell membrane; Multi-pass membrane protein. |
| Protein Families | Claudin family |
| Database Links |
HGNC: 2045 OMIM: 602910 KEGG: hsa:1365 STRING: 9606.ENSP00000378577 UniGene: Hs.647023 |
