Human Claudin-5(CLDN5) ELISA kit

Instructions
Code CSB-EL005507HU
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name claudin 5
Alternative Names Androgen withdrawal and apoptosis induced protein RVP1 like ELISA Kit; AWAL ELISA Kit; BEC 1 ELISA Kit; BEC1 ELISA Kit; Claudin 5 (transmembrane protein deleted in velocardiofacial syndrome) ELISA Kit; Claudin-5 ELISA Kit; Claudin5 ELISA Kit; CLD5_HUMAN ELISA Kit; CLDN 5 ELISA Kit; Cldn5 ELISA Kit; CPETR L1 ELISA Kit; CPETRL 1 ELISA Kit; CPETRL1 ELISA Kit; TMDVCF ELISA Kit; TMVCF ELISA Kit; Transmembrane protein deleted in VCFS ELISA Kit; Transmembrane protein deleted in velocardiofacial syndrome ELISA Kit
Abbreviation CLDN5
Uniprot No. O00501
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 31.25 pg/mL-2000 pg/mL
Sensitivity 7.8 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human CLDN5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 93  
Range % 88-97  
1:2 Average % 97  
Range % 92-102  
1:4 Average % 100  
Range % 94-107  
1:8 Average % 92  
Range % 87-99  
Recovery
The recovery of human CLDN5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 102 95-106  
EDTA plasma (n=4) 95 89-104  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
2000 1.930 2.012 1.971 1.850  
1000 1.496 1.598 1.547 1.426  
500 1.053 1.068 1.061 0.940  
250 0.732 0.756 0.744 0.623  
125 0.444 0.435 0.440 0.319  
62.5 0.312 0.326 0.319 0.198  
31.25 0.217 0.201 0.209 0.088  
0 0.122 0.120 0.121    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

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Target Data

Function Plays a major role in tight junction-specific obliteration of the intercellular space.
Gene References into Functions
  1. Results show that Claudin 5 expression is activated by SIRT1 deacetylating and potentiating KLF4. PMID: 28888043
  2. the present study revealed the distinct expression profiles of claudin5, 7 and 8 in nonneoplastic mucosal tissues and gastric carcinoma tissues. Furthermore, the expression of these claudin proteins was highly associated with metastatic progression and prognosis in patients with gastric carcinoma PMID: 29901188
  3. High CLND5 expression is associated with brain metastasis. PMID: 29328410
  4. Claudin-5 regulates blood-brain barrier permeability by modifying brain microvascular endothelial cell proliferation, migration. PMID: 28961379
  5. miR-30a-5p inhibits proliferation, metastasis, and EMT, and upregulates the expression of tight junction claudin-5 in UTUC cells. PMID: 28829370
  6. Claudin-5 was shown to be regulated VEGFR2/PI3K-Akt dependently by VEGF and PI3K-Akt independently by histamine. Interleukin-8 was shown to downregulate claudin-5 by histamine. PMID: 27873103
  7. This study shown the changes in gene expression of CDH5 and CLDN5 due to shear stress within individual differentiations also revealed no trend. PMID: 28774343
  8. Claudin-5 single-nucleotide polymorphism (SNP) rs885985 has two major alleles, G and A, which encode for glutamine (Q) or a stop signal, respectively, resulting in distinct overlapping open readings. PMID: 28445614
  9. Data show that the charge of Lys65 in claudin 1 (Cldn1) and Glu158 in claudin 3 (Cldn3), and of Gln57 in claudin 5 (Cldn5) are necessary for tight junction (TJ) strand formation. PMID: 28415153
  10. suggest that the reduction of CLDN5, 7, and 18 expression loses the suppressive ability of interaction between PDK1 and Akt and causes sustained phosphorylation of Akt, resulting in the disordered proliferation in lung squamous carcinoma cells PMID: 27884700
  11. The levels of ESM1, CLDN5, IL-1beta, IL-6, and TNF-alpha were significantly higher in the migraine attack group than in the control group PMID: 27010153
  12. analysis of the membrane driven cis interactions of claudin-5 proteins in the formation of the blood brain barrier tight junctions PMID: 26654362
  13. the expression of claudin-5 and claudin-9 was down-regulated while the expression of claudin-8 was up-regulated in cervical carcinoma tissues compared with adjacent non-neoplastic tissues. PMID: 26464708
  14. Plasma CLDN5 levels decreased in patients with stable asthma compared with those in control subjects, suggesting that asthma therapy can decrease plasma CLDN5 levels PMID: 26409663
  15. These data support that loss of claudin-5 in cardiomyocytes and endothelial cells is prevalent in human heart failure PMID: 25440958
  16. the relationship between vWF and claudin-5, which are indicators of endothelial cell dysfunction and tight junction activity, may be a predictor of disease activity in rheumatoid arthritis. PMID: 25973092
  17. The findings suggest that down regulated CLDN1 and CLDN5 genes have potential relevance in relation to the progression of glioblastoma multiforme. PMID: 25345514
  18. Claudin-5 overexpression promoted the interactions of claudin-1 and claudin-2 and enhanced the barrier function of retinal cells. PMID: 25323998
  19. Data show that C-terminal domain of Clostridium perfringens enterotoxin (cCPE)-binding to claudin 1 and claudin 5 is prevented by two residues in extracellular loop 2 of Cld1 (Asn(150) and Thr(153)) and Cld5 (Asp(149) and Thr(151)). PMID: 25342221
  20. The present study demonstrates that high expression of claudins 1, 4, 5 and down-regulation of claudin-7 are positive prognostic markers and are associated with good outcome and increased survival rates. PMID: 24815833
  21. loss of claudin 5 expression in the tumour vasculature points to a leaky vessel type PMID: 24519061
  22. The results of this study showed weak association of the CLDN5 locus with schizophrenia in Chinese. PMID: 20452046
  23. folding and assembly of CLDN3 and CLDN5 into the tight junction are controlled by non-conserved residues in the transmembrane 3 and extracellular loop 2 segments PMID: 24478310
  24. silencing of claudin-5 did significantly attenuate simvastatin-mediated endothelial cell barrier protection in response to thrombin PMID: 24028293
  25. VE-cadherin and claudin-5 were studied in nasal polyp microvessels. PMID: 23474739
  26. Matrix metalloproteinase-9 leads to claudin-5 degradation via the NF-kappaB pathway in BALB/c mice with eosinophilic meningoencephalitis caused by Angiostrongylus cantonensis. PMID: 23505411
  27. Chorionic gonadotropin induces VEGFA-dependent downregulation of claudin 5, which increases the endothelial permeability in the coculture system. PMID: 23465821
  28. Over-expression of claudin-5 was sufficient to prevent replication-deficient virus-induced permeability PMID: 23115643
  29. Claudin-5 expression and junctional organization controls intercellular barriers of human dermal microvascular but not human umbilical vein endothelial cells. PMID: 23288152
  30. Study portrays a role for Claudin-5 in cell motility involving the N-WASP signalling cascade indicating a possible role for Claudin-5 in the metastasis of human breast cancer. PMID: 22559840
  31. Letter: confirm the endothelial cell origin of cutaneous angiosarcoma and demonstrate that both endoglin and claudin-5 show promise as markers for cutaneous angiosarcoma. PMID: 23000880
  32. CLDN5 gene DNA meyhylation suggesting the pathogenesis of idiopathic pulmonary fibrosis. PMID: 22700861
  33. study demonstrated that claudin 5 is a protein with a short half-life that can undergo polyubiquitinationmainly on lysine 199, which apparently leads to its subsequent degradation PMID: 22389112
  34. this study portrays a new and interesting role for Claudin-5 in cell motility involving the N-WASP and ROCK signalling cascade PMID: 22038628
  35. GDNF increased the expression of claudin-5 and the transendothelial electrical resistance (TEER) of BMECs and PnMECs, whereas BDNF did not have this effect PMID: 22002662
  36. Galphai2 as a novel claudin-5 partner required for TJ integrity in brain endothelial cells. PMID: 22333621
  37. ERG plays a pivotal role in regulating endothelial cell barrier function and that this effect is mediated in part through its regulation of CLDN5 gene expression. PMID: 22235125
  38. Claudin-5 is a promising new marker for angiosarcomas and hemangioendotheliomas. PMID: 21959309
  39. Interendothelial claudin-5 expression depends on cerebral endothelial cell-matrix adhesion by beta(1)-integrins. PMID: 21772312
  40. This study sought to determine the cell signaling mechanism controlling endothelial CLDN5 expression during acute lung injury. PMID: 20525806
  41. tyrosine phosphorylation of claudin-5 is involved in the increased paracellular permeability of central nervous system-derived vascular endothelium induced by TGF-beta1. PMID: 21168935
  42. Claudin-1, -3, -4, -5, and -7 are expressed in developing human lung from week 12 to week 40 with distinct locations and in divergent quantities. PMID: 20478039
  43. Increased claudin-5 expression is associated with aggressive behavior in serous ovarian adenocarcinoma. PMID: 20044634
  44. Claudin 5 is transiently expressed during the development of the retinal pigment epithelium. PMID: 11944085
  45. The Blood-Brain Barrier against small molecules (<800 D), but not larger molecules, was selectively affected in Claudin-5 deficient mice. PMID: 12743111
  46. Airway tight junctions are regulated by claudin interactions that confer the selectivity of the junction. PMID: 12909588
  47. Showed that residues located C-terminal to the last transmembrane domain of claudin 5 are required for the proper targeting to apical TJ.s. PMID: 15260435
  48. CLDN5 protein may predispose to schizophrenia PMID: 15363474
  49. CLDN5 may be involved in forming a permeability barrier in the gut and, together with DQB, confer a susceptibility to schizophrenia. PMID: 15617864
  50. CLDN5 may be associated with schizophrenia in Chinese of Han descent PMID: 15820333

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Subcellular Location Cell junction, tight junction, Cell membrane, Multi-pass membrane protein
Protein Families Claudin family
Database Links

HGNC: 2047

OMIM: 602101

KEGG: hsa:7122

STRING: 9606.ENSP00000384554

UniGene: Hs.505337

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