Code | CSB-RA037143A0HU |
Size | US$210 |
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Application | Recommended Dilution |
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IHC | 1:50-1:200 |
The rabbit was immunized with a synthesized peptide from the human CLDN5 protein. Subsequently, B cells were isolated from the immunized rabbit, and RNA was extracted from these B cells. The extracted RNA was then reverse-transcribed into cDNA, serving as a template for extending CLDN5 antibody genes using degenerate primers. The resulting CLDN5 antibody genes were incorporated into a plasmid vector, followed by transfection into host cells for expression. The resulting CLDN5 recombinant monoclonal antibody was purified from the cell culture supernatant using affinity chromatography. The CLDN5 antibody was assessed for its utility in ELISA and IHC applications, with the ability to recognize human CLDN5 protein.
The CLDN5 protein, as a component of tight junctions, plays a central role in regulating the permeability of endothelial barriers, particularly in the formation and maintenance of the blood-brain barrier. Its functions are crucial for preserving tissue homeostasis and protecting the brain from potential threats.
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