Human Cluster of differentiation 147,CD147 Elisa Kit

Instructions
Code CSB-E12994h
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name basigin (Ok blood group)
Alternative Names 5A11 antigen ELISA Kit; 5F7 ELISA Kit; BASI_HUMAN ELISA Kit; Basigin (Ok blood group) ELISA Kit; Basigin ELISA Kit; Blood brain barrier HT7 antigen ELISA Kit; Bsg ELISA Kit; CD 147 ELISA Kit; CD147 ELISA Kit; CD147 antigen ELISA Kit; Collagenase stimulatory factor ELISA Kit; EMMPRIN ELISA Kit; Extracellular matrix metalloproteinase inducer ELISA Kit; Leukocyte activation antigen M6 ELISA Kit; M 6 ELISA Kit; M6 ELISA Kit; M6 leukocyte activation antigen ELISA Kit; Neurothelin ELISA Kit; OK ELISA Kit; OK blood group ELISA Kit; OK blood group antigen ELISA Kit; TCSF ELISA Kit; Tumor cell derived collagenase stimulatory factor ELISA Kit; Tumor cell-derived collagenase stimulatory factor ELISA Kit
Abbreviation BSG
Uniprot No. P35613
Species Homo sapiens (Human)
Sample Types serum, plasma
Detection Range 12.5 pg/mL-800 pg/mL
Sensitivity 3.12 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cancer
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human CD147 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 98
Range % 93-104
1:2 Average % 94
Range % 89-98
1:4 Average % 96
Range % 93-100
1:8 Average % 92
Range % 88-96
Recovery
The recovery of human CD147 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 89 85-93
EDTA plasma (n=4) 107 103-110
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
800 2.112 2.202 2.157 2.053
400 1.756 1.735 1.746 1.642
200 1.142 1.201 1.172 1.068
100 0.725 0.741 0.733 0.629
50 0.413 0.405 0.409 0.305
25 0.278 0.281 0.280 0.176
12.5 0.211 0.200 0.206 0.102
0 0.105 0.103 0.104  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Target Data

Function Plays an important role in targeting the monocarboxylate transporters SLC16A1, SLC16A3, SLC16A8 and SLC16A11 to the plasma membrane. Plays pivotal roles in spermatogenesis, embryo implantation, neural network formation and tumor progression. Stimulates adjacent fibroblasts to produce matrix metalloproteinases (MMPS). Seems to be a receptor for oligomannosidic glycans. In vitro, promotes outgrowth of astrocytic processes.
Gene References into Functions
  1. ectopic CD147-polarized distribution on basolateral membrane promotes hepatocyte depolarization by activation of the CD147-integrin alpha5beta1-E-cadherin ubiquitination-partitioning defective 3 decrease and beta-catenin translocation signaling cascade, replenishing a molecular pathway in hepatic carcinogenesis PMID: 29356040
  2. CD147 and the beta2-adrenergic receptor form hetero-oligomeric complexes.Actinin-4 directly binds to the cytosolic tail of CD147 and governs the assembly of CD147-beta2-adrenergic receptor complexes in highly ordered clusters at bacterial adhesion sites. PMID: 28569760
  3. Plasma sCD147 levels are elevated in breast cancer patients. Soluble CD147 is also associated with tumour size, lymph node metastasis, high recurrent risk, and chemoresistance. PMID: 29807951
  4. overexpression of CD147 may promote OSF progression. PMID: 29457855
  5. these findings show that CD147 is a novel and key mediator of IL-22-induced psoriatic alterations in the epidermis and might be a therapeutic target in patients with psoriasis. PMID: 28272440
  6. Data indicate that CD147 promotes breast cancer cell proliferation, metastasis, and invasion by modulating matrix metalloproteinase 9 (MMP-9) and vascular endothelial growth factor (VEGF) expression. PMID: 29901696
  7. BSG expression is indicative of a poor prognosis in pulmonary adenocarcinoma PMID: 29431238
  8. CD147 expression was associated with clinicopathological features and biomarkers related to triple-negative breast cancer, particularly basal-like breast cancer. PMID: 30099407
  9. Characterization of the role of CD147 in the development of tumors should lead to a better understanding of the changes occurring at the molecular level during the development and progression of primary human bone cancer. PMID: 30043854
  10. Results find CD147 expression level increased in colorectal cancer (CRC) tumors and cell lines and inversely correlated with mir-485 expression. Also, CD147 is a target gene of miR485 which negatively regulates its gene expression. PMID: 29532886
  11. Our results indicate that concomitant stimulation and colocalization of galectin-3 with CD147 are associated with increased gelatinolytic activity in the actively ulcerating human cornea PMID: 29340650
  12. These findings reveal an important cytomegalovirus mechanism for evading antiviral innate immunity through its encoded microRNA US25-1-5p by targeting transmembrane glycoprotein CD147. PMID: 29194430
  13. on a major part of fibroblastic cells in encapsulating peritoneal sclerosis PMID: 28551820
  14. Review: Bsg is an extracellular receptor for CyPA that promotes cell proliferation and inflammation. Thus, the CyPA/Bsg system is potentially a novel therapeutic target for cardiovascular diseases. PMID: 28993547
  15. protein-protein interaction studies on TRAF6 and BSG to get molecular level insights of the reactions. PMID: 28612997
  16. results showed that the rs4919862 SNP of CD147 was closely associated with carotid atherosclerotic plaques formation; thus, polymorphisms of the CD147 gene may be related to the tendency for carotid atherosclerotic plaques PMID: 28582638
  17. Results indicated that higher expression of CD147/EMMPRIN potentially may be a prognostic marker for most cancers. CD147/EMMPRIN overexpression predicted a high risk for chemotherapy drugs resistance. CD147/EMMPRIN is a central player in tumor progression and predicts a poor prognosis. [review] PMID: 27608940
  18. BSG mutation is associated with early-onset high myopia. PMID: 28373534
  19. Cancer cells and fibroblasts interaction promotes breast cancer cells showing stem-like cell properties through up-regulation of EMMPRIN. PMID: 27325313
  20. Cholesterol depletion inhibits the endocytosis of CD147 but that the constitutive shedding of CD147 mediated by ADAM10 is enhanced. PMID: 28703811
  21. Overexpression of CD147 support the metabolic reprograming in papillary renal cell carcinoma PMID: 28028797
  22. This research found that CD147 is correlated with aggressiveness of the glioma, tumor grades, tumor size, necrosis, overall survival, and recurrence rate. PMID: 28560663
  23. Upregulation of CD147 Promotes Metastasis of Cholangiocarcinoma by Modulating the Epithelial-to-Mesenchymal Transitional Process PMID: 28244853
  24. Our retrospective analysis demonstrated CD147 protein expression was significantly associated with clinical N stage, and tumor stage. Meanwhile, it can also serve as an independent prognosis biomarker. PMID: 28427166
  25. miR-492 may be involved in the regulation of OK antigen expression on red blood cells with the BSG rs8259 TT genotype. PMID: 28981932
  26. Our findings show that emmprin is released through microvesicle shedding in sarcoma cells, and emmprin in microvesicles regulates MMP-2 production by influencing the activity of fibroblasts located at sites distant from the tumor cells. PMID: 28498412
  27. enhancement of the effect of trastuzumab on HER2-positive cells following CD147 knockdown might be attributed to increased apoptosis and decreased phosphorylation of signaling proteins PMID: 27363028
  28. this study shows that CD147 silencing inhibits tumor growth by suppressing glucose transport in melanoma PMID: 27556188
  29. Fucosylation of CD147 by Lewis y antigen enhanced the survival ability by promoting basic autophagy activity and restraining autophagic cell death in ovarian cancer. PMID: 27863372
  30. Review/Meta-analysis: up-regulation of CD147 was effectively predictive of worse prognosis in gastrointestinal cancer. PMID: 27768590
  31. Suggest a central role for CD147 in pancreatic cancer metabolic reprogramming, particularly with respect to amino acid anabolism and calcium signaling. PMID: 28039486
  32. combined CA9-/CD147-capture antibodies demonstrated high efficiency for capturing circulating renal cell carcinoma cells PMID: 27494883
  33. important factor in the aggressive behaviour of melanoma PMID: 27060463
  34. Xkr8-BSG/NPTN complex is required for phosphatidylserine scrambling in apoptotic cell membranes. PMID: 27503893
  35. Our study provides new evidence that interaction of KLF6 and Sp1 regulates basigin-2 expression in hepatocellular carcinoma PMID: 27057625
  36. EMMPRIN/MMPs/VEGF pathway is involved in PDR angiogenesis. PMID: 27860331
  37. study identified novel interactions between CD147 and RING1, recovered CD147 nuclear envelope distribution in melanoma cells, and suggested a new mechanism underlying how cytoplasmic CD147 promotes melanoma development PMID: 28832687
  38. Immunohistochemistry was employed to analyze cFos, cJun and CD147 expression in 41 UCB cases and 34 noncancerous human bladder tissues. PMID: 28358415
  39. High BSG expression is associated with multiple myeloma. PMID: 28017969
  40. report that a phosphoserine (pSer) in CD147 (pSer252) is responsible for this interaction and inhibition of the Smad4/p21(WAF1) signal that promotes cell proliferation PMID: 28684116
  41. findings indicate that Rab22a enhances recycling of CD147, which is required for lung cancer cell migration and invasion,and targeting CD147 recycling may be a rational strategy for lung cancer therapy PMID: 28433697
  42. Studied expression of CD147 in both ALK+ and ALK- anaplastic large-cell lymphoma; found CD147 to be a downstream target for activation by C/EBPbeta. PMID: 28581487
  43. this studies identify potential signaling routes of CD147 affecting T cell growth and function PMID: 28148733
  44. Our results highlight the important role of the TGF-beta1-CD147 self-sustaining network in driving HCC development by regulating differentiation plasticity, which provides a strong basis for further investigations of the differentiation therapy of HCC targeting TGF-beta1 and CD147. PMID: 27041581
  45. rs8259 polymorphism failed to exhibit an association with cardiovascular mortality (p = 0.283). BSG rs8259 polymorphism may contribute to decreased risk of Chronic Heart Failure in a Chinese Han population PMID: 28230811
  46. This study suggests that CD44 and CD147 together improve the prognostic efficacy of tumor differentiation; in vitro results further point out that these markers might be determinant of differentiation characteristics, imparting properties of increased self-renewal, migration, and invasion. PMID: 28631562
  47. Results suggest that CD147 promotes chemoresistance by activating MAPK/ERK signaling pathway in head and neck squamous cell carcinoma (HNSCC). PMID: 28062212
  48. PvTRAg38 binds to two erythrocyte receptors basigin and band 3 through P2 and P4 regions, respectively, to facilitate parasite growth. PMID: 27881677
  49. phosphatidylinositol 3-kinase/Akt pathway may be the probable signaling pathway underlying CD147 induced up-regulation of VEGF in U937-derived foam cells PMID: 27619643
  50. D147 expression is potentially closely related to Hepatocellular Carcinoma survival and associated clinicopathological parameters, paving the way for further research PMID: 28386553
  51. Data show that FBXO22 mediated the polyubiquitination and degradation of CD147 by interacting with CD147 intracellular domain (CD147-ICD). PMID: 28117675
  52. EMMPRIN may be a valuable immunohistochemical marker for differentiating LMS from benign smooth muscle tumors in problematic cases. PMID: 26352545
  53. CD147 promotes the 5-Fluorouracil (5-FU) resistance, and MAPK/ERK signaling pathway is involved in CD147-promoted invasion and EMT of CRC cells. PMID: 25550778
  54. EMMPRIN may have a role in acute myocardial infarction and stable coronary artery disease PMID: 27439686
  55. This study identified that combination of nadroparin and irradiation had a strong synergistic antitumor effect in a dose- and time-related manner in vitro, which was reflected in the inhibition of cell viability, invasion and metastasis, promotion of apoptosis, inhibited secretion level of TGF-beta1 and downregulation of CD147, MMP-2 and survivin expression. PMID: 27498922
  56. in cultured H9C2 cells and that CyPA/CD147 signaling pathway inhibition protects against Ang II-induced cardiomyocyte hypertrophy. PMID: 27590243
  57. CD147 was significantly upregulated in the human glioma cell lines. Downregulation of CD147 suppressed cell proliferation, blocked cell cycle, induced apoptosis, inhibited cell invasion and angiogenesis in glioma cells in vitro. CD147 was significantly associated with WHO tumor grade and angiogenesis; silencing of CD147 contributed to inhibition of glioma proliferation, invasion and angiogenesis. PMID: 27761842
  58. rs11473 polymorphism at the miR- 483-5p binding site in the 3'-UTR of basigin gene may play a key role in the development of esophageal cancer in a Chinese population. PMID: 27420938
  59. findings of this research defined a novel relationship between the down-regulation of miR-22 and the up-regulation of CD147 and demonstrated that CD147 is a downstream factor of miR-22. PMID: 26943814
  60. Triptolide represses the migration and invasion of prostate cancer cells through Cav-1/CD147/MMPs signalling pathway. PMID: 27847199
  61. Basigin is important in vision, spermatogenesis and other physiological phenomena, and plays significant roles in the pathogenesis of numerous diseases, including cancer. Basigin is also the receptor for an invasive protein RH5, which is present in malaria parasites. PMID: 26684586
  62. The present study indicated that Slug and EMMPRIN are potential biomarkers and therapeutic targets for the diagnosis and treatment of perineural invasion in human salivary adenoid cystic carcinoma. PMID: 26349748
  63. High expression of EMMPRIN plays a crucial role in breast cancer cell proliferation, matrigel invasion and tumor formation. PMID: 27434292
  64. CD147 promoted the differentiation of Th17 cells by regulation of cytokine production, which provided the evidence for pathogenesis and potential therapeutic targets for rheumatoid arthritis. PMID: 27859685
  65. Study identified CD147 as a novel interaction partner for E2F1 and suggest a role in promoting tumor cell invasion and migration of prostate cancer cells. PMID: 26891801
  66. High BSG expression is associated with melanoma invasion and metastasis. PMID: 26769849
  67. High CD147 promotes liver tumor cell movement and metastasis via direct interaction with Annexin A2 and DOCK3-beta-catenin-WAVE2 signaling axis. PMID: 26716413
  68. EMMPRIN expression is involved in the development of interstitial fibrosis and tubular atrophy in human kidney allografts. PMID: 26659824
  69. High CD147 expression was significantly associated with tumorigenicity of Kaposi's sarcoma-associated herpesvirus infected endothelial cells. PMID: 26675551
  70. CD147 also participates in inflammation, nutrient and drug transporter activity, microbial pathology and developmental processes PMID: 26604323
  71. CD147 is resistant to tumor necrosis factor-induced apoptosis by promoting IKK/IkappaB/NF-kappaB pathway, and the extracellular portion of CD147 is the functional region. PMID: 26296700
  72. MCT1 expression was not clearly associated with overall or disease-free survival. MCT4 and CD147 expression correlate with worse prognosis across many cancer types. These results warrant further investigation of these associations PMID: 26779534
  73. CD147 is increased in hepatocellular carcinoma cells under starvation and reduces cell death through upregulating mTOR in vitro. PMID: 26496775
  74. EMMPRIN, MMP8, hyaluronan, beta-defensing-2, and neutrophil gelatinase-associated lipocalin (NGAL) are elevated in women with vaginal inflammation PMID: 26773532
  75. malignant proliferation of human breast cancer MCF-7 cells was significantly inhibited after silencing of the EMMPRIN gene using RNAi, and cells in the G1/G0 and G2/M phases were increased. PMID: 26634540
  76. High CD147 expression is associated with multiple myeloma. PMID: 26202926
  77. role of CD147 in the regulation of lipid metabolism, including de novo lipogenesis and fatty acid beta-oxidation, in hepatocellular carcinoma cells PMID: 26282231
  78. both EGFR and EMMPRIN proteins are potential targets for cancer therapy and EMMPRIN can be used as a prognostic marker of a more aggressive biological behavior in patients with head and neck squamous cell carcinoma PMID: 26296920
  79. The cHAb18 was produced in rNDV-18HL-infected cells followed by releasing into the supernatant by cytolysis. The rNDV-18HL-encoded cHAb18 antibody kept affinity for CD147 and showed inhibiting the migration and invasion of hepatocellular carcinoma cells PMID: 26689432
  80. these results reveal that CD147 functions as a critical regulator of endoplasmic reticulum -dependent [Ca2+]i oscillations to promote oncogenic progression in hepatocellular carcinoma PMID: 26498680
  81. Prognostic significance of CD147 protein expression could not surpass that of MCT4, especially of SLC16A3 DNA methylation, corroborating the role of MCT4 as prognostic biomarker for ccRCC. PMID: 26384346
  82. results suggest that CD147 plays an important role in colorectal cancer stem cells PMID: 26316167
  83. EMMPRIN promoted metastasis and the epithelial-mesenchymal transition in esophageal cancer cells by regulating HIF-1alpha. PMID: 26458866
  84. provide new evidence that HAb18G/CD147-mediated cell-cell contact confers anoikis resistance in an E-cadherin-dependent manner PMID: 20398401
  85. We conclude that recombinant adenovirus-mediated siRNA targeting CD147 based on the IGF2 LOI system inhibited the growth of the LOI cells in vitro and in vivo, which would provide a novel approach for targeted CRC gene therapy. PMID: 26397886
  86. The authors identified two new human proteins that interact with Ehrlichia chaffeensis EtpE-C: CD147 and heterogeneous nuclear ribonucleoprotein K (hnRNP-K). PMID: 26530384
  87. Disruption of BASIGIN in tumour cells and in MEFs has no action on the production of MMPs. PMID: 26284589
  88. Basigin facilitates the membrane distribution of CD98 to promote hepatocellular carcinoma cell spreading and tumorigenicity. PMID: 26437640
  89. Report CD147 expression in giant cell tumor of bones. PMID: 26339416
  90. Results show that CD147 expression is significantly decreased in prostate cancer tissue and is associated with tumor progression. Low CD147 expression is an independent marker that can predict biochemical recurrence after prostatectomy. PMID: 26209327
  91. There is a role of EMMPRIN in tumor cell migration through ss1 integrin/kindlin-3-mediated adhesion pathway. PMID: 25807898
  92. CD147 is a novel coreceptor of VEGFR-2 with a role in mediating its activation by VEGF PMID: 25825981
  93. employed a recombinant soluble, fully glycosylated EMMPRIN domain (rhsEMN) as a tool to characterize the structural basis of EMMPRIN-EMMPRIN receptor contacts and their functional effects on MCF-7 breast carcinoma cells PMID: 26277583
  94. interaction of rmEMMPRINex with U937 cells leads to inhibition of MCT1 membrane expression, intracellular activation of procaspase-9, followed by DNA fragmentation and apoptosis. PMID: 26024713
  95. Extracellular CyPA activates platelets via cluster of differentiation 147-mediated phosphoinositid-3-kinase/Akt-signaling, leading to enhanced adhesion and thrombus formation independently of intracellular CyPA. PMID: 25550208
  96. Data indicate that EMMPRIN is highly expressed in different regions of the mammalian brain, and is up-regulated in several central nervous system disorders. prompting targeting EMMPRIN as a measure to resolve pathology. {review] PMID: 25644103
  97. EMMPRIN (CD147) is upregulated on monocyte subsets in patients with acute myocardial infarct as compared to patients with stable coronary artery disease PMID: 25824988
  98. S100P expression in prostate cancer tissue was significantly decreased, whereas CD147 and OCT4 expression was increased. Their expression levels were closely associated with TNM stage and distant metastasis. PMID: 26125892
  99. Collectively, our results suggest that a Sp1-CD147 positive feedback loop plays a critical role in the invasion ability of ovarian cancer cells. PMID: 25998266
  100. miR-485-5p represses hepatocellular carcinoma invasive and metastatic capacities by targeting EMMPRIN expression. PMID: 26054676

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Subcellular Location Cell membrane, Single-pass type I membrane protein, Melanosome
Tissue Specificity Present only in vascular endothelium in non-neoplastic regions of the brain, whereas it is present in tumor cells but not in proliferating blood vessels in malignant gliomas.
Database Links

HGNC: 1116

OMIM: 109480

KEGG: hsa:682

STRING: 9606.ENSP00000333769

UniGene: Hs.501293

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