Human Cluster of differentiation 147,CD147 Elisa Kit

Instructions
Code CSB-E12994h
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name basigin (Ok blood group)
Alternative Names 5A11 antigen ELISA Kit; 5F7 ELISA Kit; BASI_HUMAN ELISA Kit; Basigin (Ok blood group) ELISA Kit; Basigin ELISA Kit; Blood brain barrier HT7 antigen ELISA Kit; Bsg ELISA Kit; CD 147 ELISA Kit; CD147 ELISA Kit; CD147 antigen ELISA Kit; Collagenase stimulatory factor ELISA Kit; EMMPRIN ELISA Kit; Extracellular matrix metalloproteinase inducer ELISA Kit; Leukocyte activation antigen M6 ELISA Kit; M 6 ELISA Kit; M6 ELISA Kit; M6 leukocyte activation antigen ELISA Kit; Neurothelin ELISA Kit; OK ELISA Kit; OK blood group ELISA Kit; OK blood group antigen ELISA Kit; TCSF ELISA Kit; Tumor cell derived collagenase stimulatory factor ELISA Kit; Tumor cell-derived collagenase stimulatory factor ELISA Kit
Abbreviation BSG
Uniprot No. P35613
Species Homo sapiens (Human)
Sample Types serum, plasma
Detection Range 12.5 pg/mL-800 pg/mL
Sensitivity 3.12 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cancer
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human CD147 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 98
Range % 93-104
1:2 Average % 94
Range % 89-98
1:4 Average % 96
Range % 93-100
1:8 Average % 92
Range % 88-96
Recovery
The recovery of human CD147 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 89 85-93
EDTA plasma (n=4) 107 103-110
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
800 2.112 2.202 2.157 2.053
400 1.756 1.735 1.746 1.642
200 1.142 1.201 1.172 1.068
100 0.725 0.741 0.733 0.629
50 0.413 0.405 0.409 0.305
25 0.278 0.281 0.280 0.176
12.5 0.211 0.200 0.206 0.102
0 0.105 0.103 0.104  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Data

Function Plays an important role in targeting the monocarboxylate transporters SLC16A1, SLC16A3, SLC16A8 and SLC16A11 to the plasma membrane. Plays pivotal roles in spermatogenesis, embryo implantation, neural network formation and tumor progression. Stimulates adjacent fibroblasts to produce matrix metalloproteinases (MMPS). Seems to be a receptor for oligomannosidic glycans. In vitro, promotes outgrowth of astrocytic processes.
Gene References into Functions
  1. ectopic CD147-polarized distribution on basolateral membrane promotes hepatocyte depolarization by activation of the CD147-integrin alpha5beta1-E-cadherin ubiquitination-partitioning defective 3 decrease and beta-catenin translocation signaling cascade, replenishing a molecular pathway in hepatic carcinogenesis PMID: 29356040
  2. CD147 and the beta2-adrenergic receptor form hetero-oligomeric complexes.Actinin-4 directly binds to the cytosolic tail of CD147 and governs the assembly of CD147-beta2-adrenergic receptor complexes in highly ordered clusters at bacterial adhesion sites. PMID: 28569760
  3. Plasma sCD147 levels are elevated in breast cancer patients. Soluble CD147 is also associated with tumour size, lymph node metastasis, high recurrent risk, and chemoresistance. PMID: 29807951
  4. overexpression of CD147 may promote OSF progression. PMID: 29457855
  5. these findings show that CD147 is a novel and key mediator of IL-22-induced psoriatic alterations in the epidermis and might be a therapeutic target in patients with psoriasis. PMID: 28272440
  6. Data indicate that CD147 promotes breast cancer cell proliferation, metastasis, and invasion by modulating matrix metalloproteinase 9 (MMP-9) and vascular endothelial growth factor (VEGF) expression. PMID: 29901696
  7. BSG expression is indicative of a poor prognosis in pulmonary adenocarcinoma PMID: 29431238
  8. CD147 expression was associated with clinicopathological features and biomarkers related to triple-negative breast cancer, particularly basal-like breast cancer. PMID: 30099407
  9. Characterization of the role of CD147 in the development of tumors should lead to a better understanding of the changes occurring at the molecular level during the development and progression of primary human bone cancer. PMID: 30043854
  10. Results find CD147 expression level increased in colorectal cancer (CRC) tumors and cell lines and inversely correlated with mir-485 expression. Also, CD147 is a target gene of miR485 which negatively regulates its gene expression. PMID: 29532886
  11. Our results indicate that concomitant stimulation and colocalization of galectin-3 with CD147 are associated with increased gelatinolytic activity in the actively ulcerating human cornea PMID: 29340650
  12. These findings reveal an important cytomegalovirus mechanism for evading antiviral innate immunity through its encoded microRNA US25-1-5p by targeting transmembrane glycoprotein CD147. PMID: 29194430
  13. on a major part of fibroblastic cells in encapsulating peritoneal sclerosis PMID: 28551820
  14. Review: Bsg is an extracellular receptor for CyPA that promotes cell proliferation and inflammation. Thus, the CyPA/Bsg system is potentially a novel therapeutic target for cardiovascular diseases. PMID: 28993547
  15. protein-protein interaction studies on TRAF6 and BSG to get molecular level insights of the reactions. PMID: 28612997
  16. results showed that the rs4919862 SNP of CD147 was closely associated with carotid atherosclerotic plaques formation; thus, polymorphisms of the CD147 gene may be related to the tendency for carotid atherosclerotic plaques PMID: 28582638
  17. Results indicated that higher expression of CD147/EMMPRIN potentially may be a prognostic marker for most cancers. CD147/EMMPRIN overexpression predicted a high risk for chemotherapy drugs resistance. CD147/EMMPRIN is a central player in tumor progression and predicts a poor prognosis. [review] PMID: 27608940
  18. BSG mutation is associated with early-onset high myopia. PMID: 28373534
  19. Cancer cells and fibroblasts interaction promotes breast cancer cells showing stem-like cell properties through up-regulation of EMMPRIN. PMID: 27325313
  20. Cholesterol depletion inhibits the endocytosis of CD147 but that the constitutive shedding of CD147 mediated by ADAM10 is enhanced. PMID: 28703811
  21. Overexpression of CD147 support the metabolic reprograming in papillary renal cell carcinoma PMID: 28028797
  22. This research found that CD147 is correlated with aggressiveness of the glioma, tumor grades, tumor size, necrosis, overall survival, and recurrence rate. PMID: 28560663
  23. Upregulation of CD147 Promotes Metastasis of Cholangiocarcinoma by Modulating the Epithelial-to-Mesenchymal Transitional Process PMID: 28244853
  24. Our retrospective analysis demonstrated CD147 protein expression was significantly associated with clinical N stage, and tumor stage. Meanwhile, it can also serve as an independent prognosis biomarker. PMID: 28427166
  25. miR-492 may be involved in the regulation of OK antigen expression on red blood cells with the BSG rs8259 TT genotype. PMID: 28981932
  26. Our findings show that emmprin is released through microvesicle shedding in sarcoma cells, and emmprin in microvesicles regulates MMP-2 production by influencing the activity of fibroblasts located at sites distant from the tumor cells. PMID: 28498412
  27. enhancement of the effect of trastuzumab on HER2-positive cells following CD147 knockdown might be attributed to increased apoptosis and decreased phosphorylation of signaling proteins PMID: 27363028
  28. this study shows that CD147 silencing inhibits tumor growth by suppressing glucose transport in melanoma PMID: 27556188
  29. Fucosylation of CD147 by Lewis y antigen enhanced the survival ability by promoting basic autophagy activity and restraining autophagic cell death in ovarian cancer. PMID: 27863372
  30. Review/Meta-analysis: up-regulation of CD147 was effectively predictive of worse prognosis in gastrointestinal cancer. PMID: 27768590
  31. Suggest a central role for CD147 in pancreatic cancer metabolic reprogramming, particularly with respect to amino acid anabolism and calcium signaling. PMID: 28039486
  32. combined CA9-/CD147-capture antibodies demonstrated high efficiency for capturing circulating renal cell carcinoma cells PMID: 27494883
  33. important factor in the aggressive behaviour of melanoma PMID: 27060463
  34. Xkr8-BSG/NPTN complex is required for phosphatidylserine scrambling in apoptotic cell membranes. PMID: 27503893
  35. Our study provides new evidence that interaction of KLF6 and Sp1 regulates basigin-2 expression in hepatocellular carcinoma PMID: 27057625
  36. EMMPRIN/MMPs/VEGF pathway is involved in PDR angiogenesis. PMID: 27860331
  37. study identified novel interactions between CD147 and RING1, recovered CD147 nuclear envelope distribution in melanoma cells, and suggested a new mechanism underlying how cytoplasmic CD147 promotes melanoma development PMID: 28832687
  38. Immunohistochemistry was employed to analyze cFos, cJun and CD147 expression in 41 UCB cases and 34 noncancerous human bladder tissues. PMID: 28358415
  39. High BSG expression is associated with multiple myeloma. PMID: 28017969
  40. report that a phosphoserine (pSer) in CD147 (pSer252) is responsible for this interaction and inhibition of the Smad4/p21(WAF1) signal that promotes cell proliferation PMID: 28684116
  41. findings indicate that Rab22a enhances recycling of CD147, which is required for lung cancer cell migration and invasion,and targeting CD147 recycling may be a rational strategy for lung cancer therapy PMID: 28433697
  42. Studied expression of CD147 in both ALK+ and ALK- anaplastic large-cell lymphoma; found CD147 to be a downstream target for activation by C/EBPbeta. PMID: 28581487
  43. this studies identify potential signaling routes of CD147 affecting T cell growth and function PMID: 28148733
  44. Our results highlight the important role of the TGF-beta1-CD147 self-sustaining network in driving HCC development by regulating differentiation plasticity, which provides a strong basis for further investigations of the differentiation therapy of HCC targeting TGF-beta1 and CD147. PMID: 27041581
  45. rs8259 polymorphism failed to exhibit an association with cardiovascular mortality (p = 0.283). BSG rs8259 polymorphism may contribute to decreased risk of Chronic Heart Failure in a Chinese Han population PMID: 28230811
  46. This study suggests that CD44 and CD147 together improve the prognostic efficacy of tumor differentiation; in vitro results further point out that these markers might be determinant of differentiation characteristics, imparting properties of increased self-renewal, migration, and invasion. PMID: 28631562
  47. Results suggest that CD147 promotes chemoresistance by activating MAPK/ERK signaling pathway in head and neck squamous cell carcinoma (HNSCC). PMID: 28062212
  48. PvTRAg38 binds to two erythrocyte receptors basigin and band 3 through P2 and P4 regions, respectively, to facilitate parasite growth. PMID: 27881677
  49. phosphatidylinositol 3-kinase/Akt pathway may be the probable signaling pathway underlying CD147 induced up-regulation of VEGF in U937-derived foam cells PMID: 27619643
  50. D147 expression is potentially closely related to Hepatocellular Carcinoma survival and associated clinicopathological parameters, paving the way for further research PMID: 28386553

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Subcellular Location Cell membrane, Single-pass type I membrane protein, Melanosome
Tissue Specificity Present only in vascular endothelium in non-neoplastic regions of the brain, whereas it is present in tumor cells but not in proliferating blood vessels in malignant gliomas.
Database Links

HGNC: 1116

OMIM: 109480

KEGG: hsa:682

STRING: 9606.ENSP00000333769

UniGene: Hs.501293

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