Purity
Greater than 95% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/ug as determined by LAL method.
Activity
Measured by its binding ability in a functional ELISA. Immobilized CD147 at 2 μg/ml can bind Anti-CD147 recombinant Antibody, the EC50 is 21.95-33.12 ng/ml.
Molecular Characterization
Species
Homo sapiens (Human)
Expression Region
22-205aa
Target Protein Sequence
AAGTVFTTVEDLGSKILLTCSLNDSATEVTGHRWLKGGVVLKEDALPGQKTEFKVDSDDQWGEYSCVFLPEPMGTANIQLHGPPRVKAVKSSEHINEGETAMLVCKSESVPPVTDWAWYKITDSEDKALMNGSESRFFVSSSQGRSELHIENLNMEADPGQYRCNGTSSKGSDQAIITLRVRSH
Protein Length
Partial of Isoform 2
Tag Info
C-terminal hFc-tagged
Form
Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
Buffer
Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
A part of human basigin (BSG) (22-205AA) carrying a C-terminal human IgG1 Fc tag was expressed in mammalian cells. The product is the recombinant human BSG protein. It is an active protein, whose bioactivity has been validated in a functional ELISA by binding to the anti-BSG recombinant antibody with the EC50 of 21.95-33.12 ng/ml. Its purity is greater than 95% determined by SDS-PAGE. It contains less than 1.0 EU/ug measured by the LAL method. It is in stock now so that there is no preparation waiting period.
BSG, also called CD147, is a transmembrane glycoprotein involved in reproduction, neural function, inflammation, and tumor invasion. It is also a potent inducer of MMPs and angiogenic factors such as VEGF, a chaperone for monocarboxylate transporters, or a key modulator of cell metabolism.
