Name: Recombinant Human Basigin(BSG),partial (Active)
Brand: CUSABIO
SKU: CSB-MP002831HU1

Product Details

Description	A part of human basigin (BSG) (22-205AA) carrying a C-terminal human IgG1 Fc tag was expressed in mammalian cells. The product is the recombinant human BSG protein. It is an active protein, whose bioactivity has been validated in a functional ELISA by binding to the anti-BSG recombinant antibody with the EC₅₀ of 21.95-33.12 ng/ml. Its purity is greater than 95% determined by SDS-PAGE. It contains less than 1.0 EU/ug measured by the LAL method. It is in stock now so that there is no preparation waiting period. BSG, also called CD147, is a transmembrane glycoprotein involved in reproduction, neural function, inflammation, and tumor invasion. It is also a potent inducer of MMPs and angiogenic factors such as VEGF, a chaperone for monocarboxylate transporters, or a key modulator of cell metabolism.
Purity	Greater than 95% as determined by SDS-PAGE.
Endotoxin	Less than 1.0 EU/ug as determined by LAL method.
Activity	Measured by its binding ability in a functional ELISA. Immobilized CD147 at 2 μg/ml can bind Anti-CD147 recombinant Antibody, the EC₅₀ is 21.95-33.12 ng/ml.
Target Names	BSG
Uniprot No.	P35613
Alternative Names	(CD147)(TCSF)(EMMPRIN)
Molecular Characterization
Species	Homo sapiens (Human)
Source	Mammalian cell
Expression Region	22-205aa
Target Protein Sequence	AAGTVFTTVEDLGSKILLTCSLNDSATEVTGHRWLKGGVVLKEDALPGQKTEFKVDSDDQWGEYSCVFLPEPMGTANIQLHGPPRVKAVKSSEHINEGETAMLVCKSESVPPVTDWAWYKITDSEDKALMNGSESRFFVSSSQGRSELHIENLNMEADPGQYRCNGTSSKGSDQAIITLRVRSH
Mol. Weight	49
Protein Length	Partial of Isoform 2
Tag Info	C-terminal hFc-tagged
Form	Lyophilized powder Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer	Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4
Reconstitution	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs	Protein FAQs
Storage Condition	Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life	The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time	3-7 business days
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA	Please contact us to get it.

Target Background

Function (From Uniprot)	Plays an important role in targeting the monocarboxylate transporters SLC16A1, SLC16A3, SLC16A8 and SLC16A11 to the plasma membrane. Plays pivotal roles in spermatogenesis, embryo implantation, neural network formation and tumor progression. Stimulates adjacent fibroblasts to produce matrix metalloproteinases (MMPS). Seems to be a receptor for oligomannosidic glycans. In vitro, promotes outgrowth of astrocytic processes.
Gene References into Functions	ectopic CD147-polarized distribution on basolateral membrane promotes hepatocyte depolarization by activation of the CD147-integrin alpha5beta1-E-cadherin ubiquitination-partitioning defective 3 decrease and beta-catenin translocation signaling cascade, replenishing a molecular pathway in hepatic carcinogenesis PMID: 29356040 CD147 and the beta2-adrenergic receptor form hetero-oligomeric complexes.Actinin-4 directly binds to the cytosolic tail of CD147 and governs the assembly of CD147-beta2-adrenergic receptor complexes in highly ordered clusters at bacterial adhesion sites. PMID: 28569760 Plasma sCD147 levels are elevated in breast cancer patients. Soluble CD147 is also associated with tumour size, lymph node metastasis, high recurrent risk, and chemoresistance. PMID: 29807951 overexpression of CD147 may promote OSF progression. PMID: 29457855 these findings show that CD147 is a novel and key mediator of IL-22-induced psoriatic alterations in the epidermis and might be a therapeutic target in patients with psoriasis. PMID: 28272440 Data indicate that CD147 promotes breast cancer cell proliferation, metastasis, and invasion by modulating matrix metalloproteinase 9 (MMP-9) and vascular endothelial growth factor (VEGF) expression. PMID: 29901696 BSG expression is indicative of a poor prognosis in pulmonary adenocarcinoma PMID: 29431238 CD147 expression was associated with clinicopathological features and biomarkers related to triple-negative breast cancer, particularly basal-like breast cancer. PMID: 30099407 Characterization of the role of CD147 in the development of tumors should lead to a better understanding of the changes occurring at the molecular level during the development and progression of primary human bone cancer. PMID: 30043854 Results find CD147 expression level increased in colorectal cancer (CRC) tumors and cell lines and inversely correlated with mir-485 expression. Also, CD147 is a target gene of miR485 which negatively regulates its gene expression. PMID: 29532886 Our results indicate that concomitant stimulation and colocalization of galectin-3 with CD147 are associated with increased gelatinolytic activity in the actively ulcerating human cornea PMID: 29340650 These findings reveal an important cytomegalovirus mechanism for evading antiviral innate immunity through its encoded microRNA US25-1-5p by targeting transmembrane glycoprotein CD147. PMID: 29194430 on a major part of fibroblastic cells in encapsulating peritoneal sclerosis PMID: 28551820 Review: Bsg is an extracellular receptor for CyPA that promotes cell proliferation and inflammation. Thus, the CyPA/Bsg system is potentially a novel therapeutic target for cardiovascular diseases. PMID: 28993547 protein-protein interaction studies on TRAF6 and BSG to get molecular level insights of the reactions. PMID: 28612997 results showed that the rs4919862 SNP of CD147 was closely associated with carotid atherosclerotic plaques formation; thus, polymorphisms of the CD147 gene may be related to the tendency for carotid atherosclerotic plaques PMID: 28582638 Results indicated that higher expression of CD147/EMMPRIN potentially may be a prognostic marker for most cancers. CD147/EMMPRIN overexpression predicted a high risk for chemotherapy drugs resistance. CD147/EMMPRIN is a central player in tumor progression and predicts a poor prognosis. [review] PMID: 27608940 BSG mutation is associated with early-onset high myopia. PMID: 28373534 Cancer cells and fibroblasts interaction promotes breast cancer cells showing stem-like cell properties through up-regulation of EMMPRIN. PMID: 27325313 Cholesterol depletion inhibits the endocytosis of CD147 but that the constitutive shedding of CD147 mediated by ADAM10 is enhanced. PMID: 28703811 Overexpression of CD147 support the metabolic reprograming in papillary renal cell carcinoma PMID: 28028797 This research found that CD147 is correlated with aggressiveness of the glioma, tumor grades, tumor size, necrosis, overall survival, and recurrence rate. PMID: 28560663 Upregulation of CD147 Promotes Metastasis of Cholangiocarcinoma by Modulating the Epithelial-to-Mesenchymal Transitional Process PMID: 28244853 Our retrospective analysis demonstrated CD147 protein expression was significantly associated with clinical N stage, and tumor stage. Meanwhile, it can also serve as an independent prognosis biomarker. PMID: 28427166 miR-492 may be involved in the regulation of OK antigen expression on red blood cells with the BSG rs8259 TT genotype. PMID: 28981932 Our findings show that emmprin is released through microvesicle shedding in sarcoma cells, and emmprin in microvesicles regulates MMP-2 production by influencing the activity of fibroblasts located at sites distant from the tumor cells. PMID: 28498412 enhancement of the effect of trastuzumab on HER2-positive cells following CD147 knockdown might be attributed to increased apoptosis and decreased phosphorylation of signaling proteins PMID: 27363028 this study shows that CD147 silencing inhibits tumor growth by suppressing glucose transport in melanoma PMID: 27556188 Fucosylation of CD147 by Lewis y antigen enhanced the survival ability by promoting basic autophagy activity and restraining autophagic cell death in ovarian cancer. PMID: 27863372 Review/Meta-analysis: up-regulation of CD147 was effectively predictive of worse prognosis in gastrointestinal cancer. PMID: 27768590 Suggest a central role for CD147 in pancreatic cancer metabolic reprogramming, particularly with respect to amino acid anabolism and calcium signaling. PMID: 28039486 combined CA9-/CD147-capture antibodies demonstrated high efficiency for capturing circulating renal cell carcinoma cells PMID: 27494883 important factor in the aggressive behaviour of melanoma PMID: 27060463 Xkr8-BSG/NPTN complex is required for phosphatidylserine scrambling in apoptotic cell membranes. PMID: 27503893 Our study provides new evidence that interaction of KLF6 and Sp1 regulates basigin-2 expression in hepatocellular carcinoma PMID: 27057625 EMMPRIN/MMPs/VEGF pathway is involved in PDR angiogenesis. PMID: 27860331 study identified novel interactions between CD147 and RING1, recovered CD147 nuclear envelope distribution in melanoma cells, and suggested a new mechanism underlying how cytoplasmic CD147 promotes melanoma development PMID: 28832687 Immunohistochemistry was employed to analyze cFos, cJun and CD147 expression in 41 UCB cases and 34 noncancerous human bladder tissues. PMID: 28358415 High BSG expression is associated with multiple myeloma. PMID: 28017969 report that a phosphoserine (pSer) in CD147 (pSer252) is responsible for this interaction and inhibition of the Smad4/p21(WAF1) signal that promotes cell proliferation PMID: 28684116 findings indicate that Rab22a enhances recycling of CD147, which is required for lung cancer cell migration and invasion,and targeting CD147 recycling may be a rational strategy for lung cancer therapy PMID: 28433697 Studied expression of CD147 in both ALK+ and ALK- anaplastic large-cell lymphoma; found CD147 to be a downstream target for activation by C/EBPbeta. PMID: 28581487 this studies identify potential signaling routes of CD147 affecting T cell growth and function PMID: 28148733 Our results highlight the important role of the TGF-beta1-CD147 self-sustaining network in driving HCC development by regulating differentiation plasticity, which provides a strong basis for further investigations of the differentiation therapy of HCC targeting TGF-beta1 and CD147. PMID: 27041581 rs8259 polymorphism failed to exhibit an association with cardiovascular mortality (p = 0.283). BSG rs8259 polymorphism may contribute to decreased risk of Chronic Heart Failure in a Chinese Han population PMID: 28230811 This study suggests that CD44 and CD147 together improve the prognostic efficacy of tumor differentiation; in vitro results further point out that these markers might be determinant of differentiation characteristics, imparting properties of increased self-renewal, migration, and invasion. PMID: 28631562 Results suggest that CD147 promotes chemoresistance by activating MAPK/ERK signaling pathway in head and neck squamous cell carcinoma (HNSCC). PMID: 28062212 PvTRAg38 binds to two erythrocyte receptors basigin and band 3 through P2 and P4 regions, respectively, to facilitate parasite growth. PMID: 27881677 phosphatidylinositol 3-kinase/Akt pathway may be the probable signaling pathway underlying CD147 induced up-regulation of VEGF in U937-derived foam cells PMID: 27619643 D147 expression is potentially closely related to Hepatocellular Carcinoma survival and associated clinicopathological parameters, paving the way for further research PMID: 28386553 Show More Hide All
Subcellular Location	Cell membrane, Single-pass type I membrane protein, Melanosome
Tissue Specificity	Present only in vascular endothelium in non-neoplastic regions of the brain, whereas it is present in tumor cells but not in proliferating blood vessels in malignant gliomas.
Database Links	HGNC: 1116 OMIM: 109480 KEGG: hsa:682 STRING: 9606.ENSP00000333769 UniGene: Hs.501293

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Code	CSB-MP002831HU1
Size	$368
Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel. Activity Measured by its binding ability in a functional ELISA. Immobilized CD147 at 2 μg/ml can bind Anti-CD147 recombinant Antibody, the EC₅₀ is 21.95-33.12 ng/ml. Biological Activity Assay
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Recombinant Human Basigin(BSG),partial (Active)

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Target Background

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