Human Cold-inducible RNA-binding protein(CIRBP) ELISA kit

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Target Background

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Product Details

Description

This Human CIRBP ELISA Kit was designed for the quantitative measurement of Human CIRBP protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 12.5 pg/mL-800 pg/mL and the sensitivity is 3.12 pg/mL.

Target Name

cold inducible RNA binding protein

Alternative Names

A18 hnRNP ELISA Kit; A18HNRNP ELISA Kit; cirbp ELISA Kit; CIRBP_HUMAN ELISA Kit; CIRP ELISA Kit; Cold inducible RNA binding protein ELISA Kit; Cold-inducible RNA-binding protein ELISA Kit; Glycine rich RNA binding protein ELISA Kit; Glycine-rich RNA-binding protein CIRP ELISA Kit; testicular tissue protein Li 39 ELISA Kit

Abbreviation

CIRBP

Uniprot No.

Q14011

Species

Homo sapiens (Human)

Sample Types

serum, plasma, tissue homogenates, cell lysates

Detection Range

12.5 pg/mL-800 pg/mL

Sensitivity

3.12 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Neuroscience

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human CIRBP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:5	Average %	94
	Range %	89-98
1:10	Average %	95
	Range %	91-99
1:20	Average %	89
	Range %	85-95
1:40	Average %	92
	Range %	86-99

Recovery

The recovery of human CIRBP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	84-99
EDTA plasma (n=4)	98	90-105

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
800	2.115	2.215	2.165	2.024
400	1.617	1.717	1.667	1.526
200	1.057	1.017	1.037	0.896
100	0.692	0.702	0.697	0.556
50	0.398	0.408	0.403	0.262
25	0.311	0.321	0.316	0.175
12.5	0.199	0.195	0.197	0.056
0	0.141	0.140	0.141

ELISA Data Analysis

Watch ELISA data processing video & download Curve Expert if needed

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days

Target Background

Function (From Uniprot)	Cold-inducible mRNA binding protein that plays a protective role in the genotoxic stress response by stabilizing transcripts of genes involved in cell survival. Acts as a translational activator. Seems to play an essential role in cold-induced suppression of cell proliferation. Binds specifically to the 3'-untranslated regions (3'-UTRs) of stress-responsive transcripts RPA2 and TXN. Acts as a translational repressor. Promotes assembly of stress granules (SGs), when overexpressed.
Gene References into Functions	The serum and synovial concentrations of CIRP in the rheumatoid arthritis patients were increased, suggesting that CIRP mediates inflammation and is a potential marker for synovial inflammation. PMID: 27315340 This study reports that cold-inducible RNA-binding protein (CIRBP) is a newly identified key regulator in DNA double-strand break (DSB) repair. PMID: 29432179 Crystal structure of the hnRNP A18 RNA recognition motif has been reported. PMID: 28368279 cold temperature can induce an airway inflammatory response and excess mucus production via a CIRP-mediated increase in mRNA stability and protein translation PMID: 27477308 CIRP Expression Is Induced in Skin Cancer Cells and in Keratinocytes Exposed to Lower-Dose But Not Higher-Dose UVB Radiation. PMID: 27864909 In human abdominal aortic aneurysm (AAA) tissue, Cold-inducible RNA-binding protein (CIRP) exhibited a 5.6-fold and 93% increase in mRNA and protein expression, respectively. In a rat AAA model, CIRP was upregulated significantly in a time-dependent manner in the serum and AAA tissue. PMID: 26936526 We found out that the link between CIRP and Snail is mediated by ERK and p38 pathways. EMT is a critical component of carcinoma metastasis and invasion. As demonstrated in this study, the biological role of CIRP in EMT may explain why CIRP overexpression has been associated with a bad prognosis in cancer patients. PMID: 27395339 CIRP was expressed in the bronchi of human COPD patients and was involved in inflammatory factors and MUC5AC expression after cold stimulation through the ERK and NF-kappaB pathways PMID: 27184164 Low CIRP expression is associated with colon Cancer. PMID: 28373441 this study shows that CIRP expression in bronchial airway epithelial cells of patients with chronic obstructive pulmonary disease is higher than that in healthy person PMID: 27423012 We generated a transgenic mouse model overexpressing human CIRP in the mammary epithelium to ask if it plays a role in mammary gland development. Effects of CIRP overexpression on mammary gland morphology, cell proliferation, and apoptosis were studied from puberty through pregnancy, lactation and weaning PMID: 27837912 Increased synovial fluid CIRP concentrations were closely associated with the severity of knee osteoarthritis PMID: 27840101 hnRNP A18 can promote tumor growth in in vivo models by coordinating the translation of pro-survival transcripts to support the demands of proliferating cells and increase survival under cellular stress. PMID: 26824423 Clinically, CIRP overexpression is significantly correlated with Cushing's disease recurrence. CIRP appears to play a critical tumorigenesis function in Cushing's disease and its expression might be a useful biomarker for tumor recurrence. PMID: 26824322 CIRP protein regulates telomerase activity in a temperature-dependent manner by regulating the level of TERT mRNAs. PMID: 26673712 Study shows that CIRP elevated plasma concentration is significantly associated with poor prognosis among patients with sepsis. Therefore, CIRP is a potential predictor of sepsis prognosis. PMID: 26361390 The expression of CIRP in pituitary adenoma is closely related with tumor proliferation and invasion, and its significantly elevated expression level indicates post-op recurrence. PMID: 25934796 CIRP inhibits DNA damage-induced apoptosis by regulating p53 protein.CIRP suppresses p53 upregulation during apoptosis.CIRP regulates the expression of genes involved in apoptosis. PMID: 26188505 High levels of CIRP protein expression was associated with a short survival rate in oral squamous cell carcinoma patients. PMID: 25027624 Cirp promotes the development of intestinal inflammation and colorectal tumors through regulating apoptosis and production of TNFalpha and IL23 in inflammatory cells. PMID: 25187386 report increased levels of cold-inducible RNA-binding protein (CIRP) in the blood of individuals admitted to the surgical intensive care unit with hemorrhagic shock PMID: 24097189 CIRBP regulates the expression of interleukin-1beta in an NF-kappaB-dependent fashion. PMID: 23437386 CIRBP contributes to ultraviolet light- and lipopolysaccharide-induced expression of IL1beta by regulating NFkappaB activity. PMID: 23437386 results suggest that cold inducible RNA binding protein may participate in the cell cycle regulation of normal endometrium and the loss of its expression may be involved in endometrial carcinogenesis PMID: 12819390 RBM3 and CIRP are adaptatively expressed in response to hypoxia by a mechanism that involves neither HIF-1 nor mitochondria PMID: 15075239 CIRP enhanced extracellular signal-regulated kinase 1 and 2 (ERK1/2) phosphorylation, and treatment with an MEK inhibitor decreased the proliferation caused by CIRP. PMID: 19158277 pharmacological modulation of RBM3 and CIRBP may represent novel therapeutic approaches for prostate cancer. PMID: 19277990 Show More Hide All
Subcellular Location	Nucleus, nucleoplasm. Cytoplasm.
Tissue Specificity	Ubiquitous.
Database Links	HGNC: 1982 OMIM: 602649 KEGG: hsa:1153 STRING: 9606.ENSP00000322887 UniGene: Hs.618145

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Check Code：

Code	CSB-EL005440HU
Size	96T,5×96T,10×96T
Trial Size
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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.