Human Collagen Type Ⅰ,Col Ⅰ ELISA Kit

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Human Collagen Type Ⅰ,Col Ⅰ ELISA Kit

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Protocol

Code	CSB-E08082h-IS
Size	96T,5×96T,10×96T
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Target Name

Collagen Type Ⅰ,Col Ⅰ

Alternative Names

Collagen Type 1,Col 1,Collagen Type I,Col I

Abbreviation

Col Ⅰ

Species

Homo sapiens (Human)

Sample Types

serum, plasma, cell culture supernates, saliva, tissue homogenates

Detection Range

0.24ng/mL-15ng/ml

Sensitivity

0.12ng/mL

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Signal Transduction

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human Col Ⅰ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:20	Average %	92
	Range %	88-97
1:40	Average %	100
	Range %	95-106
1:80	Average %	94
	Range %	88-99
1:160	Average %	103
	Range %	99-109

Recovery

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


ng/ml	OD1	OD2	Average	Corrected
15	3.400	3.234	3.317	3.155
7.5	2.809	2.627	2.718	2.556
3.75	1.692	1.788	1.740	1.578
1.88	1.024	1.029	1.027	0.864
0.94	0.609	0.621	0.615	0.453
0.47	0.411	0.403	0.407	0.245
0.24	0.293	0.287	0.290	0.128
0	0.168	0.157	0.163

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

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