Human Complement 1q,C1q ELISA Kit

Instructions
Code CSB-E10118h
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name complement component 1, q subcomponent, B chain
Alternative Names C1qb ELISA Kit; C1QB_HUMAN ELISA Kit; Complement C1q subcomponent subunit B ELISA Kit; Complement component 1 q subcomponent B chain ELISA Kit; Complement component 1 q subcomponent beta polypeptide ELISA Kit; Complement component C1q B chain ELISA Kit; Complement subcomponent C1q chain B ELISA Kit
Abbreviation C1QB
Uniprot No. P02746
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.156 ng/mL-10 ng/mL
Sensitivity 0.039 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%        
Three samples of known concentration were tested twenty times on one plate to assess.    
Inter-assay Precision (Precision between assays): CV%<10%        
Three samples of known concentration were tested in twenty assays to assess.      
               
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human C1q in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
  Sample Serum(n=4)    
1:1 Average % 103    
Range % 100-106    
1:2 Average % 99    
Range % 92-106    
1:4 Average % 89    
Range % 85-93    
1:8 Average % 94    
Range % 90-98    
Recovery
The recovery of human C1q spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
 
Sample Type Average % Recovery Range    
Serum (n=5) 105 102-108    
EDTA plasma (n=4) 95 90-99    
               
               
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
 
ng/ml OD1 OD2 Average Corrected    
10 2.015 1.952 1.984 1.859    
5 1.758 1.878 1.818 1.693    
2.5 1.514 1.569 1.542 1.417    
1.25 0.995 0.968 0.982 0.857    
0.625 0.614 0.632 0.623 0.498    
0.312 0.475 0.457 0.466 0.341    
0.156 0.334 0.355 0.345 0.220    
0 0.123 0.126 0.125      
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Data

Function C1q associates with the proenzymes C1r and C1s to yield C1, the first component of the serum complement system. The collagen-like regions of C1q interact with the Ca(2+)-dependent C1r(2)C1s(2) proenzyme complex, and efficient activation of C1 takes place on interaction of the globular heads of C1q with the Fc regions of IgG or IgM antibody present in immune complexes.
Gene References into Functions
  1. C1QB expression is significantly upregulated in human masticatory mucosa during wound healing PMID: 28005267
  2. We identified a major linear epitope of C1q that is the target of anti-C1q in systemic lupus erythematosus. PMID: 22740328
  3. The susceptibility for schizophrenia was particularly associated with C1QB rs291982 GG genotype. PMID: 21951915
  4. a 2-gene signature consisting of PLEK2 and C1QB led to the best result that correctly classified 93.3% melanoma patients and 90% healthy controls PMID: 21698244
  5. The C-terminal globular region of the C1Q B chain may have evolved as a functionally specialized domain or module with distinct binding properties which together with the A and C chains confers versatility and flexibility to the whole C1q molecule. PMID: 12847249
  6. Complementary interacting sites on the C1q globular domain have been precisely defined. Characterization of point mutants suggests a central role for Arg114 and a complementary role for Arg129, Arg163, and His117 of C1Q B chain in the C1q-IgG interaction. PMID: 15034050
  7. The first analysis of C1q by mass spectrometry yields evidence that the B chain moiety of the globular head is involved in the interaction with fucoidan and underscores the particular role of arginine-109 in the charge pattern recognition property of C1q. PMID: 15709773
  8. results suggest that charged residues belonging to the apex of the gC1q heterotrimer (with participation of all three chains) as well as the side of the ghB are crucial for C1q binding to ligands, IgG1, C-reactive protein, and pentraxin 3. PMID: 16566583
  9. No C1q or low molecular weight C1q was detected in sera and no anti-C1q autoantibodies were found. Sequencing of the C1q genes revealed a novel missense mutation (Gly-Arg) in codon 217 of the B chain. PMID: 17513176

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Involvement in disease Complement component C1q deficiency (C1QD)
Subcellular Location Secreted
Database Links

HGNC: 1242

OMIM: 120570

KEGG: hsa:713

STRING: 9606.ENSP00000313967

UniGene: Hs.8986

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