Product Details

Target Name

complement component 1, q subcomponent, B chain

Alternative Names

C1qb ELISA Kit; C1QB_HUMAN ELISA Kit; Complement C1q subcomponent subunit B ELISA Kit; Complement component 1 q subcomponent B chain ELISA Kit; Complement component 1 q subcomponent beta polypeptide ELISA Kit; Complement component C1q B chain ELISA Kit; Complement subcomponent C1q chain B ELISA Kit

Abbreviation

C1QB

Uniprot No.

P02746

Species

Homo sapiens (Human)

Sample Types

serum, plasma, tissue homogenates

Detection Range

0.156 ng/mL-10 ng/mL

Sensitivity

0.039 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human C1q in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	103
	Range %	100-106
1:2	Average %	99
	Range %	92-106
1:4	Average %	89
	Range %	85-93
1:8	Average %	94
	Range %	90-98

Recovery

The recovery of human C1q spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	105	102-108
EDTA plasma (n=4)	95	90-99

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
10	2.015	1.952	1.984	1.859
5	1.758	1.878	1.818	1.693
2.5	1.514	1.569	1.542	1.417
1.25	0.995	0.968	0.982	0.857
0.625	0.614	0.632	0.623	0.498
0.312	0.475	0.457	0.466	0.341
0.156	0.334	0.355	0.345	0.220
0	0.123	0.126	0.125

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

Description

This Human C1QB ELISA Kit was designed for the quantitative measurement of Human C1QB protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL.

Target Background

Function
(From Uniprot)

C1q associates with the proenzymes C1r and C1s to yield C1, the first component of the serum complement system. The collagen-like regions of C1q interact with the Ca(2+)-dependent C1r(2)C1s(2) proenzyme complex, and efficient activation of C1 takes place on interaction of the globular heads of C1q with the Fc regions of IgG or IgM antibody present in immune complexes.

Gene References into Functions

C1QB expression is significantly upregulated in human masticatory mucosa during wound healing PMID: 28005267
We identified a major linear epitope of C1q that is the target of anti-C1q in systemic lupus erythematosus. PMID: 22740328
The susceptibility for schizophrenia was particularly associated with C1QB rs291982 GG genotype. PMID: 21951915
a 2-gene signature consisting of PLEK2 and C1QB led to the best result that correctly classified 93.3% melanoma patients and 90% healthy controls PMID: 21698244
The C-terminal globular region of the C1Q B chain may have evolved as a functionally specialized domain or module with distinct binding properties which together with the A and C chains confers versatility and flexibility to the whole C1q molecule. PMID: 12847249
Complementary interacting sites on the C1q globular domain have been precisely defined. Characterization of point mutants suggests a central role for Arg114 and a complementary role for Arg129, Arg163, and His117 of C1Q B chain in the C1q-IgG interaction. PMID: 15034050
The first analysis of C1q by mass spectrometry yields evidence that the B chain moiety of the globular head is involved in the interaction with fucoidan and underscores the particular role of arginine-109 in the charge pattern recognition property of C1q. PMID: 15709773
results suggest that charged residues belonging to the apex of the gC1q heterotrimer (with participation of all three chains) as well as the side of the ghB are crucial for C1q binding to ligands, IgG1, C-reactive protein, and pentraxin 3. PMID: 16566583
No C1q or low molecular weight C1q was detected in sera and no anti-C1q autoantibodies were found. Sequencing of the C1q genes revealed a novel missense mutation (Gly-Arg) in codon 217 of the B chain. PMID: 17513176

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Involvement in disease

Complement component C1q deficiency (C1QD)

Subcellular Location

Secreted.

Database Links

HGNC: 1242

OMIM: 120570

KEGG: hsa:713

STRING: 9606.ENSP00000313967

UniGene: Hs.8986

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Code	CSB-E10118h
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Human Complement 1q,C1q ELISA Kit

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