Human Complement C1q-like protein 3(C1QL3) ELISA kit

Code CSB-EL003644HU
Size 96T,5×96T,10×96T
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Product Details

Target Name
complement component 1, q subcomponent-like 3
Alternative Names
C1q and tumor necrosis factor-related protein 13 ELISA Kit; C1q/TNF-related protein 13 ELISA Kit; C1ql ELISA Kit; C1QL3 ELISA Kit; C1QL3_HUMAN ELISA Kit; C1QTNF13 ELISA Kit; Complement C1q-like protein 3 ELISA Kit; Complement component 1; q subcomponent-like 3 ELISA Kit; CTRP13 ELISA Kit; K100 ELISA Kit
Abbreviation
C1QL3
Uniprot No.
Species
Homo sapiens (Human)
Sample Types
serum, plasma, tissue homogenates
Detection Range
0.312 ng/mL-20 ng/mL
Sensitivity
0.078 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Others
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human C1QL3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %105
Range %100-110
1:2Average %99
Range %95-107
1:4Average %89
Range %85-93
1:8Average %84
Range %80-88
Recovery
The recovery of human C1QL3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9590-100
EDTA plasma (n=4)9085-95
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
202.510 2.350 2.430 2.292
101.961 1.856 1.909 1.771
51.276 1.324 1.300 1.162
2.50.799 0.825 0.812 0.674
1.250.453 0.427 0.440 0.302
0.6250.347 0.372 0.360 0.222
0.3120.262 0.259 0.261 0.123
00.141 0.135 0.138
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Human C1QL3 ELISA Kit was designed for the quantitative measurement of Human C1QL3 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL.

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Target Background

Function
(From Uniprot)
May regulate the number of excitatory synapses that are formed on hippocampus neurons. Has no effect on inhibitory synapses. Plays a role in glucose homeostasis. Via AMPK signaling pathway, stimulates glucose uptake in adipocytes, myotubes and hepatocytes and enhances insulin-stimulated glucose uptake. In a hepatoma cell line, reduces the expression of gluconeogenic enzymes G6PC1 and PCK1 and hence decreases de novo glucose production.
Gene References into Functions
  1. CTRP13 is a novel adipokine that is down-regulated in the serum of type 2 diabetics. PMID: 28182339
  2. decreased levels of CTRP3 and especially CTRP13 were associated with increased risk of T2DM and CAD PMID: 28033351
  3. CTRP13 reduces glucose output in hepatocytes by inhibiting the mRNA expression of gluconeogenic enzymes, glucose-6-phosphatase and the cytosolic form of phosphoenolpyruvate carboxykinase. PMID: 21378161
Subcellular Location
Secreted.
Tissue Specificity
Highly expressed in adipose tissue, with expression levels at least 2 orders of magnitude higher than in other tissues, including brain and kidney.
Database Links

HGNC: 19359

OMIM: 615227

KEGG: hsa:389941

UniGene: Hs.676792

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