Human Complement fragment 3a,C3a ELISA Kit

Instructions
Code CSB-E08509h
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name Complement fragment 3a,C3a
Alternative Names N/A
Abbreviation C3a
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 31.25 ng/mL-2000 ng/mL
Sensitivity 7.81 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human C3a in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %92
Range %88-99
1:2Average %95
Range %90-105
1:4Average %92
Range %82-105
1:5Average %95
Range %90-101
Recovery
The recovery of human C3a spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9795-100
EDTA plasma (n=4)9895-102
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
20002.769 2.777 2.773 2.597
10002.374 2.381 2.378 2.202
5001.801 1.790 1.796 1.620
2501.041 1.094 1.068 0.892
1250.632 0.652 0.642 0.466
62.50.461 0.473 0.467 0.291
31.250.259 0.284 0.272 0.096
00.175 0.176 0.176  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Citations

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