Mouse Complement fragment 3a,C3a ELISA Kit

Code CSB-E08511m
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Lead Time 3-5 working days
Abbreviation C3a
Target Name Complement fragment 3a,C3a
Species Mus musculus (Mouse)
Sample Types serum, plasma, cell culture supernates, tissue homogenates
Detection Range 0.156 ng/mL-10 ng/mL
Sensitivity 0.039 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
To assess the linearity of the assay, samples were spiked with high concentrations of mouse C3a in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:10 Average % 93  
Range % 89-100  
1:20 Average % 96  
Range % 91-101  
1:40 Average % 99  
Range % 93-105  
1:80 Average % 88  
Range % 82-95  
The recovery of mouse C3a spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 98 95-104  
EDTA plasma (n=4) 93 88-99  
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
10 2.638 2.693 2.666 2.495  
5 2.224 2.132 2.178 2.007  
2.5 1.403 1.388 1.396 1.225  
1.25 0.863 0.829 0.846 0.675  
0.625 0.587 0.566 0.577 0.406  
0.312 0.453 0.432 0.443 0.272  
0.156 0.299 0.275 0.287 0.116  
0 0.164 0.178 0.171    
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