Human Complement fragment 3b,C3b ELISA Kit

Code CSB-E09303h
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name Complement fragment 3b,C3b
Abbreviation C3b
Species Homo sapiens (Human)
Sample Types serum, urine, cerebrospinal fluid (CSF), saliva
Detection Range 3.12 ng/mL-200 ng/mL
Sensitivity 0.78 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human C3b in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %87
Range %82-92
1:2Average %98
Range %92-103
1:4Average %99
Range %93-103
1:8Average %94
Range %89-98
Recovery
The recovery of human C3b spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9590-98
EDTA plasma (n=4)9791-99
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
2002.115 2.003 2.059 1.932
1001.748 1.736 1.742 1.615
501.245 1.266 1.256 1.129
250.756 0.782 0.769 0.642
12.50.568 0.572 0.570 0.443
6.250.333 0.342 0.338 0.211
3.120.199 0.187 0.193 0.066
00.128 0.126 0.127  
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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