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| Code | CSB-E14214h |
| Size | 96T,5×96T,10×96T |
| Price | Request a Quote or Start an on-line Chat |
| Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now |
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
| To assess the linearity of the assay, samples were spiked with high concentrations of human CBG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 95 | ||||
| Range % | 91-100 | |||||
| 1:2 | Average % | 93 | ||||
| Range % | 88-99 | |||||
| 1:4 | Average % | 93 | ||||
| Range % | 89-100 | |||||
| 1:8 | Average % | 97 | ||||
| Range % | 91-108 | |||||
| The recovery of human CBG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 92-99 | ||||
| EDTA plasma (n=4) | 92 | 89-95 | ||||
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||
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This Human SERPINA6 ELISA Kit was designed for the quantitative measurement of Human SERPINA6 protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 20 pmol/mL-8000 pmol/mL and the sensitivity is 20 pmol/mL .
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I am interested in measuring Corticosteroid Binding Globulin in human plasma samples. I had a look at your product CSB-E14214h. I have some questions:
1. Is the kit specific for the intact cbg or there may be cross-reactivity with the elastase-cleaved form? Is a single or double antibody design? Where do the antibodies bind the protein?
2. What are the typical plasma levels detected by your kit?
3. We need to measure about 160 samples, therefore, how many such ELISA kits do we need?
The product CSB-E14214h is not a double antibody kit. The antibody information is as below:
one is a goat anti-rabbit secondary antibody that has been pre-coated, and one is a specific antibody-rabbit polyclonal antibody. It is a competitive assay but not a traditional double antibody sandwich method. The specific binding site is unclear, and the CBG protein can be detected.
If you want to test 160 samples, if both the sample and the standard are made as single wells, two 96T can be fine. However, considering the sample and possible pre-experimental requirements and accuracy factors, it is recommended that the samples be duplicated. This will require more kits. For more details, please contact us, and let's discuss it together.
