Name: Human Cystatin-F(CST7)ELISA kit
Brand: CUSABIO
SKU: CSB-E17504h

Product Details

Target Name

cystatin F (leukocystatin)

Alternative Names

CMAP ELISA Kit; CST 7 ELISA Kit; Cst7 ELISA Kit; Cystatin 7 ELISA Kit; cystatin F (leukocystatin) ELISA Kit; Cystatin like metastasis associated protein ELISA Kit; Cystatin-7 ELISA Kit; Cystatin-F ELISA Kit; Cystatin-like metastasis-associated protein ELISA Kit; Cystatin7 ELISA Kit; CystatinF ELISA Kit; CYTF_HUMAN ELISA Kit; dJ568C11.1 ELISA Kit; Leukocystatin ELISA Kit

Abbreviation

CST7

Uniprot No.

O76096

Species

Homo sapiens (Human)

Sample Types

serum, plasma, cell culture supernates

Detection Range

0.625 ng/mL-40 ng/mL

Sensitivity

0.156 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Cell Biology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human CST7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	93
	Range %	85-97
1:2	Average %	98
	Range %	91-101
1:4	Average %	94
	Range %	85-97
1:8	Average %	90
	Range %	84-95

Recovery

The recovery of human CST7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	100	94-107
EDTA plasma (n=4)	106	98-112

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
40	2.319	2.235	2.277	2.124
20	1.685	1.671	1.678	1.525
10	0.978	0.927	0.953	0.800
5	0.641	0.627	0.634	0.481
2.5	0.355	0.369	0.362	0.209
1.25	0.247	0.260	0.254	0.101
0.625	0.195	0.188	0.192	0.039
0	0.151	0.155	0.153

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

Description

This Human CST7 ELISA Kit was designed for the quantitative measurement of Human CST7 protein in serum, plasma, cell culture supernates. It is a Sandwich ELISA kit, its detection range is 0.625 ng/mL-40 ng/mL and the sensitivity is 0.156 ng/mL.

Customer Reviews and Q&A

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■ Q&A

Does your kit # CSB-E17504h or any of its components contain recombinant proteins?
If so, what is the expression system of these recombinants?

Thanks for your inquiry!
CSB-E17504h contains recombinant proteins.
Standard is 293 cell-derived recombinant protein.

Target Background

Function
(From Uniprot)

Inhibits papain and cathepsin L but with affinities lower than other cystatins. May play a role in immune regulation through inhibition of a unique target in the hematopoietic system.

Gene References into Functions

during monocyte to macrophage differentiation, the endosomal/lysosomal proteolytic activity can be regulated by cystatin F whose expression is under the control of transcriptional factor C/EBP alpha. PMID: 30033148
Regulation of cathepsins S and L by cystatin F during maturation of dendritic cells. PMID: 22365146
Transgenic cystatin F is expressed in model mice only during demyelination; in chronic demyelination, remyelinating ability is lost. PMID: 21344476
cystatin F is an intracellular cysteine peptidase inhibitor with readily regulated expression PMID: 12423348
CST7 is secreted, but artificial modification of its C-terminus can induce its endocytic targeting. PMID: 15212960
Cystatin F was not colocalized with cystatin C shich suggests distinct functions for these two cysteine protease inhibitors in U937 cells. PMID: 15752368
analysis of reduction-dependent activation of human cystatin F PMID: 16601115
A latent protease inhibitor which is itself regulated by proteolysis in the endocytic pathway. PMID: 18256700
Data demonstrate the addition of N-linked sugars to an Asn-X-Cys motif in cystatin F and suggest that the mannose 6-phosphate sorting machinery is used to divert cystatin F from the secretory pathway and to mediate its uptake from extracellular pools. PMID: 19192250

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Subcellular Location

Secreted. Cytoplasm.

Protein Families

Cystatin family

Tissue Specificity

Primarily expressed in peripheral blood cells and spleen.

Database Links

HGNC: 2479

OMIM: 603253

KEGG: hsa:8530

STRING: 9606.ENSP00000420384

UniGene: Hs.143212

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Code	CSB-E17504h
Size	96T,5×96T,10×96T
Price	Request a Quote or Start an on-line Chat
Trial Size	24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Human Cystatin-F(CST7)ELISA kit

Product Details

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Customer Reviews and Q&A

Target Background