Human Cystatin-F(CST7)ELISA kit

Instructions
Code CSB-E17504h
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name cystatin F (leukocystatin)
Alternative Names CMAP ELISA Kit; CST 7 ELISA Kit; Cst7 ELISA Kit; Cystatin 7 ELISA Kit; cystatin F (leukocystatin) ELISA Kit; Cystatin like metastasis associated protein ELISA Kit; Cystatin-7 ELISA Kit; Cystatin-F ELISA Kit; Cystatin-like metastasis-associated protein ELISA Kit; Cystatin7 ELISA Kit; CystatinF ELISA Kit; CYTF_HUMAN ELISA Kit; dJ568C11.1 ELISA Kit; Leukocystatin ELISA Kit
Abbreviation CST7
Uniprot No. O76096
Species Homo sapiens (Human)
Sample Types serum, plasma, cell culture supernates
Detection Range 0.625 ng/mL-40 ng/mL
Sensitivity 0.156 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cell Biology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human CST7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 93
Range % 85-97
1:2 Average % 98
Range % 91-101
1:4 Average % 94
Range % 85-97
1:8 Average % 90
Range % 84-95
Recovery
The recovery of human CST7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 100 94-107
EDTA plasma (n=4) 106 98-112
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected
40 2.319 2.235 2.277 2.124
20 1.685 1.671 1.678 1.525
10 0.978 0.927 0.953 0.800
5 0.641 0.627 0.634 0.481
2.5 0.355 0.369 0.362 0.209
1.25 0.247 0.260 0.254 0.101
0.625 0.195 0.188 0.192 0.039
0 0.151 0.155 0.153  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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 Q&A
Q:

Does your kit # CSB-E17504h or any of its components contain recombinant proteins?
If so, what is the expression system of these recombinants?

A:
Thanks for your inquiry!
CSB-E17504h contains recombinant proteins.
Standard is 293 cell-derived recombinant protein.

Target Data

Function Inhibits papain and cathepsin L but with affinities lower than other cystatins. May play a role in immune regulation through inhibition of a unique target in the hematopoietic system.
Gene References into Functions
  1. during monocyte to macrophage differentiation, the endosomal/lysosomal proteolytic activity can be regulated by cystatin F whose expression is under the control of transcriptional factor C/EBP alpha. PMID: 30033148
  2. Regulation of cathepsins S and L by cystatin F during maturation of dendritic cells. PMID: 22365146
  3. Transgenic cystatin F is expressed in model mice only during demyelination; in chronic demyelination, remyelinating ability is lost. PMID: 21344476
  4. cystatin F is an intracellular cysteine peptidase inhibitor with readily regulated expression PMID: 12423348
  5. CST7 is secreted, but artificial modification of its C-terminus can induce its endocytic targeting. PMID: 15212960
  6. Cystatin F was not colocalized with cystatin C shich suggests distinct functions for these two cysteine protease inhibitors in U937 cells. PMID: 15752368
  7. analysis of reduction-dependent activation of human cystatin F PMID: 16601115
  8. A latent protease inhibitor which is itself regulated by proteolysis in the endocytic pathway. PMID: 18256700
  9. Data demonstrate the addition of N-linked sugars to an Asn-X-Cys motif in cystatin F and suggest that the mannose 6-phosphate sorting machinery is used to divert cystatin F from the secretory pathway and to mediate its uptake from extracellular pools. PMID: 19192250

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Subcellular Location Secreted, Cytoplasm
Protein Families Cystatin family
Tissue Specificity Primarily expressed in peripheral blood cells and spleen.
Database Links

HGNC: 2479

OMIM: 603253

KEGG: hsa:8530

STRING: 9606.ENSP00000420384

UniGene: Hs.143212

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