Human Cytoskeleton-associated protein 4(CKAP4) ELISA kit

Instructions
Code CSB-EL005453HU
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name cytoskeleton-associated protein 4
Alternative Names 63 kDa membrane protein ELISA Kit; CKAP 4 ELISA Kit; Ckap4 ELISA Kit; CKAP4_HUMAN ELISA Kit; Cytoskeleton associated protein 4 ELISA Kit; Cytoskeleton-associated protein 4 ELISA Kit; ERGIC 63 ELISA Kit; ERGIC63 ELISA Kit; MGC99554 ELISA Kit; p63 ELISA Kit; Transmembrane protein 63kD endoplasmic reticulum Golgi intermediate compartment ELISA Kit; Type II transmembrane protein p63 ELISA Kit
Abbreviation CKAP4
Uniprot No. Q07065
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 23.44 pg/mL-1500 pg/mL
Sensitivity 5.86 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cancer
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human CKAP4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 86
Range % 83-89
1:2 Average % 95
Range % 91-99
1:4 Average % 87
Range % 83-90
1:8 Average % 99
Range % 92-103
Recovery
The recovery of human CKAP4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 86 83-89
EDTA plasma (n=4) 100 95-105
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
1500 2.345 2.401 2.373 2.200
750 2.013 2.112 2.063 1.890
375 1.754 1.704 1.729 1.556
187.5 1.334 1.304 1.319 1.146
93.75 0.812 0.831 0.822 0.649
46.88 0.503 0.512 0.508 0.335
23.44 0.346 0.338 0.342 0.169
0 0.172 0.174 0.173  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

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Target Background

Function
(From Uniprot)
High-affinity epithelial cell surface receptor for APF.; FUNCTION
Gene References into Functions
  1. Both DKK1 and CKAP4 are frequently expressed in pancreatic and lung tumours, and their simultaneous expression is negatively correlated with prognosis. PMID: 28514532
  2. CKAP4 has a role as a Dickkopf1 receptor and in pancreatic and lung tumor progression PMID: 27322059
  3. Human Prostate Basal cell hyperplasia is an expansion of p63. PMID: 28795417
  4. APF binds specifically to sites within the cytoskeleton-associated protein 4 (CKAP4) extracellular domain PMID: 28893174
  5. The still rudimentary information of how CLIMP-63 fulfills these different roles, what these are exactly and how post-translational modifications control them, will be discussed. PMID: 25849921
  6. Although VIMP can interact with CLIMP-63 and Syn5L, it does not interact with MT-binding ER proteins (such as Reep1) that shape the tubular smooth ER PMID: 25008318
  7. revealed that CKAP4 could associate with EGFR at basal status and the complex was reduced upon EGF stimulation, leading to release EGFR into cytoplasm PMID: 24838946
  8. Single nucleotide polymorphisms in p63 are implicated in the etiology of nonsyndromic bladder-exstrophy-epispadias complex. PMID: 23913486
  9. Here we report that the combination of p63, a master regulator of epidermal development and differentiation, and KLF4, a regulator of epidermal differentiation, is sufficient to convert dermal fibroblasts to a keratinocyte phenotype. PMID: 23921950
  10. CKAP4 was correlated with favorable clinical outcome and was an independent predictor for overall survival in hepatic cholangiocarcinoma patients. PMID: 23665508
  11. p63 positively regulates desmosome adhesion by directly controlling the expression of several desmosome genes, including Dsp, Dsc3 and Dsg1. PMID: 23108156
  12. CLIMP63 regulates the luminal diameter within the endoplasmic reticulum and may play a role in regulating the formation of endoplasmic reticulum sheets. PMID: 21111237
  13. The results define CLIMP-63 as a novel protein interactor and regulator of Dicer function, involved in maintaining Dicer protein levels in human cells. PMID: 23047949
  14. Treatment of airway epitheial cells (AEC) with SP-A, monoclonal antibodies to CKAP4/P63, or CKAP4/P63-specific small interfering RNA decreased the binding of purified alginate exopolysaccharide to AEC. PMID: 22966120
  15. This study identifies specific changes in skin structural biology and signalling pathways that result from mutant p63 and provides new molecular insight into the AEC syndrome phenotype PMID: 22329826
  16. CCN2 regulation by antiproliferative factor involves CKAP4 nuclear translocation and binding to the CCN2 promoter. PMID: 22438586
  17. Tumor markers, p63 and high molecular weight cytokeratin, may be utilised in the distinction between urothelial carcinoma with prostatic stromal invasion and urothelial carcinoma with colonisation of prostatic ducts and acini. PMID: 22406481
  18. These findings strongly support the routine use of p40 in place of p63 for the diagnosis of pulmonary squamous cell carcinoma. PMID: 22056955
  19. This study found a consistent expression profile and localization analysis of p63 and GM1 in primary keratinocytes and in human epidermal biopsies. PMID: 21820419
  20. Telomere length in Sjogren syndrome is shorter and associated with lower levels of expression of p63 and nucleostemin than in non-Sjogren syndrome. PMID: 21655359
  21. Synthetic as-antiproliferative factor inhibits cell proliferation in T24 bladder carcinoma cells via the CKAP4 receptor PMID: 21143984
  22. Data demonstrate an important role for the PI3-kinase-Akt pathway in intracellular transport of surfactant protein A receptor P63. PMID: 20870746
  23. p63 binds to an enhancer element in the SHFM1 locus and this element controls expression of DLX6 and DLX5 which are important for limb development. PMID: 20808887
  24. p63 was not found to be highly sensitive for squamous cell carcinoma PMID: 20184665
  25. I510T mutation of p63 was detected in both RHS and AEC syndrome patients and mutation of S541 residue can lead to either RHS (S541Y) or AEC syndrome (S541F). PMID: 20156774
  26. evidence to support the hypothesis that p63 is the functional tPA binding site on VSMC PMID: 12913003
  27. The ectrodactyly, absence of radius side part palm and split foot malformation are caused by the mutation of base pair at number 665 of the exon 5 of P63. PMID: 15476176
  28. CLIMP-63-mediated stable anchoring of the endoplasmic reticulum (ER) to microtubules is required to maintain the spatial distribution of the ER during interphase. PMID: 15703217
  29. is not responsible for autosomal-dominant amelogenesis imperfecta PMID: 16546853
  30. cytoskeleton-associated protein 4/p63 (CKAP4/p63), a type II transmembrane receptor, binds with high affinity to APF PMID: 17030514
  31. Olomoucine, a CDK inhibitor, up-regulates CKAP4, a cytoskeleton-linking membrane protein. PMID: 17975794
  32. Identification of CKAP4/p63 as a substrate of DHHC2, a putative tumor suppressor. PMID: 18296695
  33. CK MNF116 and p63 were useful in identifying squamous cell carcinomas with single cell infiltration. PMID: 18333895
  34. Results substantiate the role of P63 as a lung surfactant protein-A receptor protein localized on the surface of lung type II cells. PMID: 18708633
  35. DHHC2-mediated palmitoylation of CKAP4 has a role in opposing cancer-related cellular behaviors. PMID: 19144824
  36. p73 and p63, but not p53, are modulated during the cell cycle PMID: 19861536
  37. The p62/p63 protein was first identified because it is palmitoylated during mitosis. PMID: 1730740
  38. Purification and cloning of p62/p63. PMID: 8566419

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Subcellular Location Endoplasmic reticulum membrane, Single-pass type II membrane protein, Cell membrane, Single-pass type II membrane protein, Cytoplasm, cytoskeleton, Cytoplasm, perinuclear region
Database Links

HGNC: 16991

KEGG: hsa:10970

STRING: 9606.ENSP00000367265

UniGene: Hs.74368

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