CKAP4, also known as cytoskeleton-associated protein 4, has alternative names including p63, CLIMP63, and ERGIC-63. CKAP4 is a type II transmembrane protein primarily localized on the cell membrane and rough endoplasmic reticulum. It serves as a receptor for various ligands and plays a crucial role in stabilizing protein structures. CKAP4 is involved in numerous biological activities, including cell proliferation, cell migration, keratinocyte differentiation, glycan synthesis, fibrosis, thymus development, heart formation, neuronal apoptosis, and cancer progression. CKAP4 and its isoforms bind to DKK1 or DKK3 (Dickkopf protein) or anti-proliferative factor (APF), regulating several downstream signaling cascades such as the PI3K/AKT and MAPK1/3 pathways. The CKAP4 complex plays a key role in signal pathway regulation and is a potential target for cancer therapy.
CKAP4 Antibody (CSB-PA005453LA01HU)
Validated Data
Western Blot
Positive WB detected in: HepG2 whole cell lysate, Hela whole cell lysate
All lanes: CKAP4 antibody at 2.7µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 67 kDa
Observed band size: 67 kDa
Immunofluorescence staining of Hela cells with CSB-PA005453LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Human Cytoskeleton-associated protein 4(CKAP4) ELISA kit (CSB-EL005453HU)
Validated Data
Code: CSB-EL005453HU
Size: 96T,5×96T,10×96T
Sensitivity: 5.86 pg/mL
Detection Range: 23.44 pg/mL-1500 pg/mL
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
The following CKAP4 reagents supplied by CUSABIO are manufactured under a strict quality control system. Multiple applications have been validated and solid technical support is offered.
CKAP4 Antibodies for Homo sapiens (Human)
Code | Product Name | Species Reactivity | Application |
---|---|---|---|
CSB-PA005453GA01HU | CKAP4 Antibody | Human,Mouse,Rat | ELISA,WB,IHC,IF |
CSB-PA133590 | CKAP4 Antibody | Human,Mouse | ELISA,WB,IHC |
CSB-PA378255 | CKAP4 Antibody | Human,Mouse | ELISA,WB,IHC |
CSB-PA005453LA01HU | CKAP4 Antibody | Human | ELISA, WB, IF |
CSB-PA005453LB01HU | CKAP4 Antibody, HRP conjugated | Human | ELISA |
CSB-PA005453LD01HU | CKAP4 Antibody, Biotin conjugated | Human | ELISA |
CKAP4 Antibodies for Mus musculus (Mouse)
Code | Product Name | Species Reactivity | Application |
---|---|---|---|
CSB-PA005453ZA01MO | Ckap4 Antibody | Mus musculus | ELISA, WB (ensure identification of antigen) |
CKAP4 Proteins for Mus musculus (Mouse)
Code | Product Name | Source |
---|---|---|
CSB-YP005453MO CSB-EP005453MO-B |
Recombinant Mouse Cytoskeleton-associated protein 4 (Ckap4), partial | Yeast In Vivo Biotinylation in E.coli |
CSB-EP005453MO | Recombinant Mouse Cytoskeleton-associated protein 4 (Ckap4), partial | E.coli |
CSB-BP005453MO | Recombinant Mouse Cytoskeleton-associated protein 4 (Ckap4), partial | Baculovirus |
CSB-MP005453MO | Recombinant Mouse Cytoskeleton-associated protein 4 (Ckap4), partial | Mammalian cell |
CKAP4 ELISA Kit for Homo sapiens (Human)
Code | Product Name | Sample Types | Sensitivity |
---|---|---|---|
CSB-EL005453HU | Human Cytoskeleton-associated protein 4(CKAP4) ELISA kit | serum, plasma, tissue homogenates, cell lysates | 5.86 pg/mL |