Human Ecto-NOX disulfide-thiol exchanger 2(ENOX2) ELISA kit

Instructions
Code CSB-EL007677HU
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name ecto-NOX disulfide-thiol exchanger 2
Alternative Names APK1 ELISA Kit; APK1 antigen ELISA Kit; COVA1 ELISA Kit; Cytosolic ovarian carcinoma antigen 1 ELISA Kit; Ecto NOX disulfide thiol exchanger 2 ELISA Kit; ENOX 2 ELISA Kit; ENOX2 ELISA Kit; ENOX2_HUMAN ELISA Kit; OTTHUMP00000024021 ELISA Kit; OTTHUMP00000024022 ELISA Kit; Protein disulfide-thiol oxidoreductase ELISA Kit; tNOX ELISA Kit; Tumor associated hydroquinone oxidase ELISA Kit; Tumor-associated hydroquinone oxidase ELISA Kit
Abbreviation ENOX2
Uniprot No. Q16206
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 31.25 pg/mL-2000 pg/mL
Sensitivity 7.81 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Neuroscience
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human ENOX2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 93  
Range % 82-97  
1:2 Average % 98  
Range % 92-104  
1:4 Average % 102  
Range % 96-108  
1:8 Average % 89  
Range % 82-95  
Recovery
The recovery of human ENOX2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 94 90-98  
EDTA plasma (n=4) 88 82-92  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
2000 2.314 2.428 2.371 2.213  
1000 1.497 1.586 1.542 1.384  
500 0.937 0.882 0.910 0.752  
250 0.567 0.553 0.560 0.402  
125 0.365 0.376 0.371 0.213  
62.5 0.271 0.264 0.268 0.110  
31.25 0.218 0.214 0.216 0.058  
0 0.157 0.158 0.158    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 5-7 working days

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Target Data

Function May be involved in cell growth. Probably acts as a terminal oxidase of plasma electron transport from cytosolic NAD(P)H via hydroquinones to acceptors at the cell surface. Hydroquinone oxidase activity alternates with a protein disulfide-thiol interchange/oxidoreductase activity which may control physical membrane displacements associated with vesicle budding or cell enlargement. The activities oscillate with a period length of 22 minutes and play a role in control of the ultradian cellular biological clock.
Gene References into Functions
  1. The tumor-associated NADH oxidase (tNOX)-mediated modulation of the NAD+ concentration and SIRT1 are involved in oxaliplatin-induced apoptosis. PMID: 28122359
  2. These findings not only shed light on the molecular mechanism of the anticancer properties of capsaicin, but also the transcription regulation of tNOX expression that may potentially explain how POU3F2 is associated with tumorigenesis. PMID: 27271588
  3. The findings support a role for arNOX as a major source of oxidative damage leading to cross-linking of skin proteins. PMID: 24906676
  4. The results suggest that phosphorylation of serine-504 by PKCdelta modulates the biological function of tNOX. PMID: 22659163
  5. this result suggests that hnRNP F directs formation of the exon 4 minus variant of ENOX2. PMID: 21625959
  6. increased NADH levels resulting from ENOX2 inhibition result in decreased prosurvival sphingosine-1-phosphate and increased proapoptotic ceramide, both of which may be important to initiation of the ENOX2 inhibitor-induced apoptotic cascade. PMID: 21571040
  7. ENOX2 is a dimeric protein containing 4 coppers/dimer capable of carrying out concerted four electron transfers from NADH or ubiquinol to molecular oxygen as required to form water. PMID: 20922471
  8. arNOX activity correlates with age and reaches a maximum at about age 65 in males and 55 in females. PMID: 20345278
  9. Enhanced arNOX activity correlates with age and with oxidative changes contributing to skin aging. PMID: 19734125
  10. tNOX is both necessary and sufficient for the cellular anticancer activities attributed to both EGCg and capsaicin. *tNOX enzyme PMID: 15706060
  11. results indicate that shRNA targeting of tNOX inhibits the growth of cervical cancer cells, and reduces cell migration via a decrease in the membrane association of Rac. PMID: 18023414
  12. A relationship of tNOX to unregulated growth of cancer cells was provided by data where growth of HeLa cells was inhibited by transfection with the exon 5 antisense oligonucleotides PMID: 18351130
  13. These results indicate that tNOX is suppressed during apoptosis and demonstrate that tNOX down-regulation sensitizes cells to stress-induced growth reduction, suggesting that tNOX is required for transformed cell growth. PMID: 18789934

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Subcellular Location Cell membrane, Secreted, extracellular space
Protein Families ENOX family
Tissue Specificity Found in the sera of cancer patients with a wide variety of cancers including breast, prostate, lung and ovarian cancers, leukemias, and lymphomas. Not found in the serum of healthy volunteers or patients with disorders other than cancer. Probably shed in
Database Links

HGNC: 2259

OMIM: 300282

KEGG: hsa:10495

STRING: 9606.ENSP00000337146

UniGene: Hs.171458

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