Human Ecto-NOX disulfide-thiol exchanger 2(ENOX2) ELISA kit

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Target Background

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Product Details

Description

This Human ENOX2 ELISA Kit was designed for the quantitative measurement of Human ENOX2 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.81 pg/mL.

Target Name

ecto-NOX disulfide-thiol exchanger 2

Alternative Names

APK1 ELISA Kit; APK1 antigen ELISA Kit; COVA1 ELISA Kit; Cytosolic ovarian carcinoma antigen 1 ELISA Kit; Ecto NOX disulfide thiol exchanger 2 ELISA Kit; ENOX 2 ELISA Kit; ENOX2 ELISA Kit; ENOX2_HUMAN ELISA Kit; OTTHUMP00000024021 ELISA Kit; OTTHUMP00000024022 ELISA Kit; Protein disulfide-thiol oxidoreductase ELISA Kit; tNOX ELISA Kit; Tumor associated hydroquinone oxidase ELISA Kit; Tumor-associated hydroquinone oxidase ELISA Kit

Abbreviation

ENOX2

Uniprot No.

Q16206

Species

Homo sapiens (Human)

Sample Types

serum, plasma, tissue homogenates, cell lysates

Detection Range

31.25 pg/mL-2000 pg/mL

Sensitivity

7.81 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Neuroscience

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human ENOX2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	93
	Range %	82-97
1:2	Average %	98
	Range %	92-104
1:4	Average %	102
	Range %	96-108
1:8	Average %	89
	Range %	82-95

Recovery

The recovery of human ENOX2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-98
EDTA plasma (n=4)	88	82-92

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2000	2.314	2.428	2.371	2.213
1000	1.497	1.586	1.542	1.384
500	0.937	0.882	0.910	0.752
250	0.567	0.553	0.560	0.402
125	0.365	0.376	0.371	0.213
62.5	0.271	0.264	0.268	0.110
31.25	0.218	0.214	0.216	0.058
0	0.157	0.158	0.158

ELISA Data Analysis

Watch ELISA data processing video & download Curve Expert if needed

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days

Target Background

Function (From Uniprot)	May be involved in cell growth. Probably acts as a terminal oxidase of plasma electron transport from cytosolic NAD(P)H via hydroquinones to acceptors at the cell surface. Hydroquinone oxidase activity alternates with a protein disulfide-thiol interchange/oxidoreductase activity which may control physical membrane displacements associated with vesicle budding or cell enlargement. The activities oscillate with a period length of 22 minutes and play a role in control of the ultradian cellular biological clock.
Gene References into Functions	The tumor-associated NADH oxidase (tNOX)-mediated modulation of the NAD+ concentration and SIRT1 are involved in oxaliplatin-induced apoptosis. PMID: 28122359 These findings not only shed light on the molecular mechanism of the anticancer properties of capsaicin, but also the transcription regulation of tNOX expression that may potentially explain how POU3F2 is associated with tumorigenesis. PMID: 27271588 The findings support a role for arNOX as a major source of oxidative damage leading to cross-linking of skin proteins. PMID: 24906676 The results suggest that phosphorylation of serine-504 by PKCdelta modulates the biological function of tNOX. PMID: 22659163 this result suggests that hnRNP F directs formation of the exon 4 minus variant of ENOX2. PMID: 21625959 increased NADH levels resulting from ENOX2 inhibition result in decreased prosurvival sphingosine-1-phosphate and increased proapoptotic ceramide, both of which may be important to initiation of the ENOX2 inhibitor-induced apoptotic cascade. PMID: 21571040 ENOX2 is a dimeric protein containing 4 coppers/dimer capable of carrying out concerted four electron transfers from NADH or ubiquinol to molecular oxygen as required to form water. PMID: 20922471 arNOX activity correlates with age and reaches a maximum at about age 65 in males and 55 in females. PMID: 20345278 Enhanced arNOX activity correlates with age and with oxidative changes contributing to skin aging. PMID: 19734125 tNOX is both necessary and sufficient for the cellular anticancer activities attributed to both EGCg and capsaicin. *tNOX enzyme PMID: 15706060 results indicate that shRNA targeting of tNOX inhibits the growth of cervical cancer cells, and reduces cell migration via a decrease in the membrane association of Rac. PMID: 18023414 A relationship of tNOX to unregulated growth of cancer cells was provided by data where growth of HeLa cells was inhibited by transfection with the exon 5 antisense oligonucleotides PMID: 18351130 These results indicate that tNOX is suppressed during apoptosis and demonstrate that tNOX down-regulation sensitizes cells to stress-induced growth reduction, suggesting that tNOX is required for transformed cell growth. PMID: 18789934 Show More Hide All
Subcellular Location	Cell membrane. Secreted, extracellular space. Note=Extracellular and plasma membrane-associated.
Protein Families	ENOX family
Tissue Specificity	Found in the sera of cancer patients with a wide variety of cancers including breast, prostate, lung and ovarian cancers, leukemias, and lymphomas. Not found in the serum of healthy volunteers or patients with disorders other than cancer. Probably shed in
Database Links	HGNC: 2259 OMIM: 300282 KEGG: hsa:10495 STRING: 9606.ENSP00000337146 UniGene: Hs.171458

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Check Code：

Code	CSB-EL007677HU
Size	96T,5×96T,10×96T
Trial Size
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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.