Recombinant Human Ecto-NOX disulfide-thiol exchanger 2 (ENOX2),partial

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Code CSB-EP007677HU1
Size US$2632Purchase it in Cusabio online store
(only available for customers from the US)
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP007677HU1 could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) ENOX2.
  • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP007677HU1 could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) ENOX2.
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Product Details

Purity Greater than 85% as determined by SDS-PAGE.
Target Names ENOX2
Uniprot No. Q16206
Research Area Cancer
Alternative Names APK1; APK1 antigen; COVA1; Cytosolic ovarian carcinoma antigen 1; Ecto NOX disulfide thiol exchanger 2; ENOX 2; ENOX2; ENOX2_HUMAN; OTTHUMP00000024021; OTTHUMP00000024022; Protein disulfide-thiol oxidoreductase; tNOX; Tumor associated hydroquinone oxidase; Tumor-associated hydroquinone oxidase
Species Homo sapiens (Human)
Source E.coli
Expression Region 314-610aa
Target Protein Sequence MEEAKEKFKQALSGILIQFEQIVAVYHSASKQKAWDHFTKAQRKNISVWCKQAEEIRNIHNDELMGIRREEEMEMSDDEIEEMTETKETEESALVSQAEALKEENDSLRWQLDAYRNEVELLKQEQGKVHREDDPNKEQQLKLLQQALQGMQQHLLKVQEEYKKKEAELEKLKDDKLQVEKMLENLKEKESCASRLCASNQDSEYPLEKTMNSSPIKSEREALLVGIISTFLHVHPFGASIEYICSYLHRLDNKICTSDVECLMGRLQHTFKQEMTGVGASLEKRWKFCGFEGLKLT
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight 34.6 kDa
Protein Length Partial
Tag Info Tag-Free
Form Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time 3-7 business days
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

Customer Reviews and Q&A

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 Q&A
Q:

I ordered this product before and have some concerns.
I am wondering if there is any polyethylene glycol that could be in the storage solution to help solubilize the protein or left over in the solution as part of the purification process or even attached to the peptide to help solubilization. We are running some Mass Spec experiments and we are seeing a signal that may indicate a PEG linkage somewhere in the protein or solution. Any help would be greatly appreciated.
Best regards !

A:
Thanks for your feedback.
Regarding this question, I further checked with our technical department, pls check the reply from them listed as below:
We used renaturation of inclusion body for this protein. In the process of renaturation, PEG 4000 was used at a final concentration of 0.2%.
However, after multiple dialysis, the final buffer after dialysis was: 10 mM Tris-HCl, 1 mM EDTA, pH 8.0, 10% glycerol, mass spectrometry detection sensitivity is very high,
there should be a very small amount of PEG 4000 residue. Could you pls let us know your specific experimental application ? Does it cause an impact on your experiment if there is any residual PEG 4000 ? If yes, pls let us know.
Q:

Just to clarify, we are running some intact protein LC-ESI-MS. The mass spectrum that we see for the intact protein looks more like a PEG signal. We are trying to find good sensitive means for detecting the protein, and want to make sure we are detecting the right species

A:
Thanks for your feedback, PEG 4000 was indeed used during the process of renaturation.
Probably there is still some residual PEG 4000 after multiple dialysis, that's why you detected the PEG signal.
If you have any other problem during the use of this protein, pls feel free to feedback to us.

Target Background

Function
May be involved in cell growth. Probably acts as a terminal oxidase of plasma electron transport from cytosolic NAD(P)H via hydroquinones to acceptors at the cell surface. Hydroquinone oxidase activity alternates with a protein disulfide-thiol interchange/oxidoreductase activity which may control physical membrane displacements associated with vesicle budding or cell enlargement. The activities oscillate with a period length of 22 minutes and play a role in control of the ultradian cellular biological clock.
Gene References into Functions
  1. The tumor-associated NADH oxidase (tNOX)-mediated modulation of the NAD+ concentration and SIRT1 are involved in oxaliplatin-induced apoptosis. PMID: 28122359
  2. These findings not only shed light on the molecular mechanism of the anticancer properties of capsaicin, but also the transcription regulation of tNOX expression that may potentially explain how POU3F2 is associated with tumorigenesis. PMID: 27271588
  3. The findings support a role for arNOX as a major source of oxidative damage leading to cross-linking of skin proteins. PMID: 24906676
  4. The results suggest that phosphorylation of serine-504 by PKCdelta modulates the biological function of tNOX. PMID: 22659163
  5. this result suggests that hnRNP F directs formation of the exon 4 minus variant of ENOX2. PMID: 21625959
  6. increased NADH levels resulting from ENOX2 inhibition result in decreased prosurvival sphingosine-1-phosphate and increased proapoptotic ceramide, both of which may be important to initiation of the ENOX2 inhibitor-induced apoptotic cascade. PMID: 21571040
  7. ENOX2 is a dimeric protein containing 4 coppers/dimer capable of carrying out concerted four electron transfers from NADH or ubiquinol to molecular oxygen as required to form water. PMID: 20922471
  8. arNOX activity correlates with age and reaches a maximum at about age 65 in males and 55 in females. PMID: 20345278
  9. Enhanced arNOX activity correlates with age and with oxidative changes contributing to skin aging. PMID: 19734125
  10. tNOX is both necessary and sufficient for the cellular anticancer activities attributed to both EGCg and capsaicin. *tNOX enzyme PMID: 15706060
  11. results indicate that shRNA targeting of tNOX inhibits the growth of cervical cancer cells, and reduces cell migration via a decrease in the membrane association of Rac. PMID: 18023414
  12. A relationship of tNOX to unregulated growth of cancer cells was provided by data where growth of HeLa cells was inhibited by transfection with the exon 5 antisense oligonucleotides PMID: 18351130
  13. These results indicate that tNOX is suppressed during apoptosis and demonstrate that tNOX down-regulation sensitizes cells to stress-induced growth reduction, suggesting that tNOX is required for transformed cell growth. PMID: 18789934

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Subcellular Location Cell membrane. Secreted, extracellular space. Note=Extracellular and plasma membrane-associated.
Protein Families ENOX family
Tissue Specificity Found in the sera of cancer patients with a wide variety of cancers including breast, prostate, lung and ovarian cancers, leukemias, and lymphomas. Not found in the serum of healthy volunteers or patients with disorders other than cancer. Probably shed in
Database Links

HGNC: 2259

OMIM: 300282

KEGG: hsa:10495

STRING: 9606.ENSP00000337146

UniGene: Hs.171458

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