Recombinant Human Ecto-NOX disulfide-thiol exchanger 2 (ENOX2),partial

In Stock
Code CSB-EP007677HU1
Product Type Recombinant Protein
Size US$2632
Uniprot No. Q16206
Relevance May be involved in cell growth. Probably acts as a terminal oxidase of plasma electron transport from cytosolic NAD(P)H via hydroquinones to acceptors at the cell surface. Hydroquinone oxidase activity alternates with a protein disulfide-thiol interchange/oxidoreductase activity which may control physical membrane displacements associated with vesicle budding or cell enlargement. The activities oscillate with a period length of 22 minutes and play a role in control of the ultradian cellular biological clock.
Image
  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP007677HU1 could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) ENOX2.
  • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP007677HU1 could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) ENOX2.
Storage Buffer Tris-based buffer,50% glycerol
Species Homo sapiens (Human)
Purity Greater than 85% as determined by SDS-PAGE.
Sequence MEEAKEKFKQALSGILIQFEQIVAVYHSASKQKAWDHFTKAQRKNISVWCKQAEEIRNIHNDELMGIRREEEMEMSDDEIEEMTETKETEESALVSQAEALKEENDSLRWQLDAYRNEVELLKQEQGKVHREDDPNKEQQLKLLQQALQGMQQHLLKVQEEYKKKEAELEKLKDDKLQVEKMLENLKEKESCASRLCASNQDSEYPLEKTMNSSPIKSEREALLVGIISTFLHVHPFGASIEYICSYLHRLDNKICTSDVECLMGRLQHTFKQEMTGVGASLEKRWKFCGFEGLKLT
Research Area Cancer
Source E.coli
Gene Names ENOX2
Protein Names APK1 antigen Cytosolic ovarian carcinoma antigen 1 Tumor-associated hydroquinone oxidase
Expression Region 314-610aa
Tag Info Tag-Free
Mol. Weight 34.6 kDa
Protein Description Partial
Storage The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
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Function May be involved in cell growth. Probably acts as a terminal oxidase of plasma electron transport from cytosolic NAD(P)H via hydroquinones to acceptors at the cell surface. Hydroquinone oxidase activity alternates with a protein disulfide-thiol interchange/oxidoreductase activity which may control physical membrane displacements associated with vesicle budding or cell enlargement. The activities oscillate with a period length of 22 minutes and play a role in control of the ultradian cellular biological clock.
Subcellular Location Cell membrane, Secreted, extracellular space
Protein Families ENOX family
Tissue Specificity Found in the sera of cancer patients with a wide variety of cancers including breast, prostate, lung and ovarian cancers, leukemias, and lymphomas. Not found in the serum of healthy volunteers or patients with disorders other than cancer. Probably shed in
Database Links

HGNC: 2259

OMIM: 300282

KEGG: hsa:10495

STRING: 9606.ENSP00000337146

UniGene: Hs.171458

Q&A and Customer Reviews

Q&A
Q:

I ordered this product before and have some concerns.
I am wondering if there is any polyethylene glycol that could be in the storage solution to help solubilize the protein or left over in the solution as part of the purification process or even attached to the peptide to help solubilization. We are running some Mass Spec experiments and we are seeing a signal that may indicate a PEG linkage somewhere in the protein or solution. Any help would be greatly appreciated.
Best regards !

A:
Thanks for your feedback.
Regarding this question, I further checked with our technical department, pls check the reply from them listed as below:
We used renaturation of inclusion body for this protein. In the process of renaturation, PEG 4000 was used at a final concentration of 0.2%.
However, after multiple dialysis, the final buffer after dialysis was: 10 mM Tris-HCl, 1 mM EDTA, pH 8.0, 10% glycerol, mass spectrometry detection sensitivity is very high,
there should be a very small amount of PEG 4000 residue. Could you pls let us know your specific experimental application ? Does it cause an impact on your experiment if there is any residual PEG 4000 ? If yes, pls let us know.
Q:

Just to clarify, we are running some intact protein LC-ESI-MS. The mass spectrum that we see for the intact protein looks more like a PEG signal. We are trying to find good sensitive means for detecting the protein, and want to make sure we are detecting the right species

A:
Thanks for your feedback, PEG 4000 was indeed used during the process of renaturation.
Probably there is still some residual PEG 4000 after multiple dialysis, that's why you detected the PEG signal.
If you have any other problem during the use of this protein, pls feel free to feedback to us.
Customer Reviews

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