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| Code | CSB-E10360h |
| Size | 96T,5×96T,10×96T |
| Price | Request a Quote or Start an on-line Chat |
| Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now |
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
| To assess the linearity of the assay, samples were spiked with high concentrations of human eotaxin 2/CCL24 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 95 | |||||
| Range % | 90-100 | ||||||
| 1:2 | Average % | 89 | |||||
| Range % | 85-94 | ||||||
| 1:4 | Average % | 96 | |||||
| Range % | 91-101 | ||||||
| 1:8 | Average % | 104 | |||||
| Range % | 100-108 | ||||||
| The recovery of human eotaxin 2/CCL24 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 99 | 95-103 | |||||
| EDTA plasma (n=4) | 86 | 83-89 | |||||
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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This human CCL24 ELISA kit employs the quantitative sandwich enzyme immunoassay technique to measure the levels of human CCL24 in multiple samples, including serum, plasma, or tissue homogenates. It also uses the enzyme-substrate chromogenic reaction to visualize and analyze the analyte levels through the color intensity. The intensity of the colored product is in direct proportion to the CCL24 levels in the sample and is measured at 450 nm through a microplate reader.
CCL24, also known as eotaxin2, mainly chemoattracts eosinophils. It can promote the separation of eosinophils from endothelial cells and then combine with its sole ligand to promote eosinophils to enter the tissue. CCL24 promotes cell trafficking and regulates inflammatory and fibrotic activities mainly through the CCR3. CCL24 induces chemotaxis and activation of CCR3-expressing cells, including immune cells and fibroblasts. A study found that CCL24 stimulated human lung fibroblast proliferation and collagen synthesis and was associated with the progression of idiopathic pulmonary fibrosis. Suppressing the expression of CCL24 can reduce airway inflammation. Macrophages can also selectively induce the production of CCL24.
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