Human Fibrinogen alpha chain (FGA) ELISA kit

Instructions
Code CSB-EL008607HU
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
Have Questions? Leave a Message or Start an on-line Chat

Product Details

Target Name Fibrinogen alpha chain (FGA)
Alternative Names FGA ELISA Kit; FGB ELISA Kit; FGG ELISA Kit; Fib2 ELISA Kit; FIBA_HUMAN ELISA Kit; Fibrinogen A alpha polypeptide ELISA Kit; Fibrinogen alpha chain ELISA Kit; Fibrinogen B alpha polypeptide ELISA Kit; Fibrinogen beta chain ELISA Kit; Fibrinogen G alpha polypeptide ELISA Kit; Fibrinogen gamma chain ELISA Kit; fibrinogen; B beta polypeptide ELISA Kit; fibrinogen; G gamma polypeptide ELISA Kit; fibrinogen; gamma polypeptide ELISA Kit; Fibrinogen--alpha -polypeptide chain ELISA Kit; Fibrinogen--beta -polypeptide chain ELISA Kit; Fibrinogen--gamma-polypeptide chain ELISA Kit
Abbreviation FGA
Uniprot No. P02671
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.94 μg/mL-60 μg/mL
Sensitivity 0.47 μg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Assay Principle quantitative
Measurement Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human FGA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:100 Average % 84  
Range % 80-92  
1:200 Average % 101  
Range % 95-105  
1:400 Average % 98  
Range % 94-102  
1:800 Average % 93  
Range % 86-98  
Recovery
The recovery of human FGA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 95 89-99  
EDTA plasma (n=4) 97 90-101  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
μg/ml OD1 OD2 Average    
60 0.080 0.082 0.081    
30 0.183 0.176 0.180    
15 0.399 0.404 0.402    
7.5 0.676 0.639 0.658    
3.75 1.256 1.224 1.240    
1.88 1.554 1.590 1.572    
0.94 1.785 1.829 1.807    
0 2.149 2.097 2.123    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Target Data

Function Cleaved by the protease thrombin to yield monomers which, together with fibrinogen beta (FGB) and fibrinogen gamma (FGG), polymerize to form an insoluble fibrin matrix. Fibrin has a major function in hemostasis as one of the primary components of blood clots. In addition, functions during the early stages of wound repair to stabilize the lesion and guide cell migration during re-epithelialization. Was originally thought to be essential for platelet aggregation, based on in vitro studies using anticoagulated blood. However, subsequent studies have shown that it is not absolutely required for thrombus formation in vivo. Enhances expression of SELP in activated platelets via an ITGB3-dependent pathway. Maternal fibrinogen is essential for successful pregnancy. Fibrin deposition is also associated with infection, where it protects against IFNG-mediated hemorrhage. May also facilitate the immune response via both innate and T-cell mediated pathways.
Gene References into Functions
  1. this study shows that hypofibrinogenaemia associated with novel Aalpha126Val-->Asp mutation in the fibrinogen coiled coil PMID: 28816342
  2. All the exons and exon-intron boundaries of fibrinogen genes (FGA, FGB and FGG) were analysed by direct DNA sequencing PMID: 29748775
  3. Taken together, an increased pretreatment plasma fibrinogen level was related to worse survival in digestive system tumors, indicating that it could be a useful prognostic marker in these types of tumors. PMID: 29874984
  4. The risk of early pregnancy loss was significantly higher in women with homozygous or heterozygous FGA: p.Thr331Ala polymorphism than in women with FGA: p.Thr331 (Odds ratio: 8.84, P < .05). PMID: 27099386
  5. Data defined the mutational burden of the fibrinogen FGA, FGB, and FGG genes, and estimated the prevalence of inherited fibrinogen disorders through a systematic analysis of exome/genome data. PMID: 29240685
  6. CD47 expression is decreased on the surface of erythrocytes in obese subjects. These changes in CD47 expression on the erythrocytes surface may be an adaptive response to hyperfibrinogenemia associated with obesity. PMID: 25914268
  7. Fibrinogen A alpha arginine-glycine-aspartate sequence (residues 95-97) is essential for binding to its erythrocyte receptor. PMID: 29662311
  8. Binding of human fibrinogen to MRP enhances Streptococcus suis survival in host blood in a alphaXbeta2 integrin-dependent manner. PMID: 27231021
  9. High fibrinogen level is associated with Type-2 diabetes and diabetic vascular disease. PMID: 28119309
  10. The heterozygous AalphaArg16His and gammaAsp185Asn identified in the study probably underlie the dysfibrinogenemia in this pedigree, with the latter being identified for the first time. PMID: 28425010
  11. Preoperative fibrinogen levels appeared as an independent mortality risk factor in non-metastatic colorectal cancer patients with normal GPS scores PMID: 28269756
  12. evidence that VLITL confers amyloidogenic properties to Aalpha-chain frameshift variants, yielding a previously unknown molecular basis for the pathogenesis of Aalpha-chain amyloidosis PMID: 29089309
  13. We used mass spectrometry of plasma from trauma patients to find that fibrinogen Aalpha-C domain methionine sulfoxide content was selectively-increased in patients with coagulopathy vs. those without coagulopathy. This evidence supports a novel linkage between oxidative stress, coagulopathy, and bleeding after injury PMID: 27105953
  14. Using a quantum chemical approach method, it has been established that mutations in MMP-10 and FGA proteins led to substantial energetic modifications suggesting an impact on their functions and/or stability in the recurrent pregnancy loss patients. PMID: 29016666
  15. Data indicate that repeated measurement after five hours showed a statistical significant difference for the fractional synthesis rate of fibrinogen but not for albumin. PMID: 28350862
  16. Data indicate only the highest concentration of apixaban was capable to significantly reduce thrombus formation, fibrin association and platelet-aggregate formation. PMID: 28192448
  17. Fibrinogen A alpha-chain amyloidosis appears to be an under-recognized disorder in Braga, Portugal, where we found a high frequency of the FGA p.Glu545Val variant PMID: 28359658
  18. High fibrinogen expression is associated with pancreatic carcinoma. PMID: 28219450
  19. The high preoperative peripheral serum fibrinogen level was related to poor survival in penile cancer patients, suggeting fibrinogen may serve as a powerful predictor of cancer specific survival (CSS) in penile cancer patients. PMID: 27738342
  20. Q237 is rapidly crosslinked first by FXIIIa followed by Q366 and Q328. Both (15)NH4Cl and (15)N-GEE could be crosslinked to the three glutamines in alphaC (233-425) with a similar order of reactivity. No glutamine is dependent on another to react first. Moreover, the remaining two glutamines of each mutant were both still reactive. These glutamines play distinct roles in fibrin crosslinking and clot architecture. PMID: 26951791
  21. The molecular characterization of two novel afibrinogenemic mutations in the fibrinogen A alpha and fibrinogen B beta chain establishes the importance of the A alpha and B beta C-terminal region for normal fibrinogen assembly and secretion. PMID: 27164460
  22. This meta-analysis results did not show significant associations between the polymorphisms in FGA and Ischemic stroke. PMID: 27266621
  23. there was a relatively high frequency of pregnancy loss in the setting of the FGA Thr312Ala polymorphism. PMID: 26139837
  24. Fibrinogen was significantly increased in pediatric patients with complicated appendicitis compared to patients with uncomplicated appendicitis or non-specific abdominal pain. PMID: 27760719
  25. No association between three single nucleotide polymorphisms in the fibrinogen alpha gene and schizophrenia. PMID: 28203040
  26. Fibrinogen Shanghai results in N-terminal truncation of Aalpha chain, which does not interfere with synthesis, assembly or secretion of fibrinogen, but compromises fibrin polymerization and clot formation. PMID: 27555433
  27. We found a mutation that consists of a R458C substitution on the fibrinogen alpha chain gene confirmed in 13 new familial subjects that causes a rare subtype of dysfibrinogenemia characterized by venous thromboembolic events PMID: 26581183
  28. Fibrinogen Nijmegen, a mutation at the same position, is causative for thrombosis, whereas fibrinogen Innsbruck appears to lead to a bleeding tendency, illustrating that even mutations at the same position can cause contrary symptoms. PMID: 26540126
  29. Neutrophilic asthma patients and eosinophilic asthma patients had increased fibrinogen compared to controls. PMID: 27044366
  30. To novel fibrinogen mutations have been identified (Aa17GlyalphaCys and Aa381SeralphaPhe) that cosegregate with dysfibrinogenemia in seven family members of a Chinese pedigree. PMID: 26083984
  31. The study reports two novel mutations detected in the FGA and FGB genes in two patients with inherited hypofibrinogenemia. PMID: 25981141
  32. Nutrient overload-induced up-regulation of all three fibrinogen component subunits of the coagulation cascade provides a possible mechanism to explain the excess cardiovascular disease mortality observed in non-alcoholic fatty liver disease patients. PMID: 26256740
  33. No association between cognition and gene polymorphisms involved in thrombosis and haemostasis PMID: 26228839
  34. An analysis of the results obtained in this study and a critical review of previously published data indicate that gene polymorphisms in FGA and FGB are not related to ischemic stroke in children PMID: 25555432
  35. Results support the idea that fibrinogen alphaC regions are involved in the thickening of fibrin fibers. PMID: 25393591
  36. The study identified a 5.9 kDa C-terminal fragment of the fibrinogen alpha chain as an early serum biomarker of fibrogenic processes in patients with liver disease. PMID: 25275549
  37. The Aalpha-Val360 related cross-sectionally to physiologic, radiologic, and symptomatic markers of disease severity of PiZZ alpha1-antitrypsin deficiency. PMID: 25569856
  38. Case Report: hepatocytes homozygous for a large FGA deletion can be genetically modified to restore Aalpha-chain protein expression and secrete a functional fibrinogen hexamer. PMID: 25163824
  39. High plasma D-dimer, fibrinogen, and platelet levels are associated with epithelial ovarian cancer independently of venous thromboembolism. PMID: 25347092
  40. Elevated pre-operative plasma fibrinogen level is associated with aggressive soft-tissue sarcoma. PMID: 24122271
  41. Results show that oxidized fibrinogen, instead of serum albumin (HSA), is the key protein for intermolecular dityrosine formation in plasma. PMID: 25280629
  42. Plasma fibrinogen is an independent predictor for the severity of coronary artery disease in diabetic patients. PMID: 24803720
  43. findings indicate that FGA and SLC6A4 gene interactions may contribute to the phenotypes of ASD rather than the incidence of ASD. PMID: 24192574
  44. role in regulation of clot formation and fibrinolysis PMID: 24048413
  45. Both crude and multivariable regression analyses show that the FGA Thr312Ala polymorphism is a protective factor in the Polish population under dominant and additive models of inheritance. PMID: 24821635
  46. Data indicate that ibrinogen binding to Staphylococcus aureus fibronectin-binding protein A (FnBPA) is sterically regulated by fibronectin binding. PMID: 24627488
  47. Stepwise assembly of fibrinogen is assisted by the endoplasmic reticulum ERp57 lectin-chaperone system in a human hepatocyte cell line. PMID: 24040290
  48. Studies indicate that in afibrinogenemia and hypofibrinogenemia, most mutations of the FGA, FGB, or FGG fibrinogen encoding genes are null mutations. PMID: 23852822
  49. Elevated plasma fibrinogen levels are associated with nonmetastatic colon cancer. PMID: 23612884
  50. These results indicate that DPT can modify certain biological functions of fibrin, and thus a another function of this extracellular matrix protein was revealed. PMID: 23877568
  51. Fibrinogen Aalpha Thr312Ala (A/G) polymorphism was associated with thromboembolic pulmonary hypertension PMID: 23894515
  52. We identify 23 robustly associated fibrinogen loci, 15 of which are new, but no support for a causal relationship between circulating levels of fibrinogen and coronary artery disease, stroke, or venous thromboembolism. PMID: 23969696
  53. These findings indicate that yellow fever virus infection does affect the transcriptional and translational expression of fibrinogen and PAI-1 in human hepatocytes. PMID: 23639427
  54. R554L fibrinogen Aalpha-chain mutation is associated with hereditary amyloidosis. PMID: 23551149
  55. human serum albumin influences glycation of fibrinogen PMID: 23958299
  56. High serum fibrinogen alpha is associated with lung squamous cell carcinoma. PMID: 23725126
  57. AalphaQ328 and AalphaQ366 are important for normal fibrin clot formation and in the absence of residues AalphaQ328 and AalphaQ366, other Gln residues in the alpha chain can support FXIIIa-catalysed fibrin cross-linking. PMID: 23224113
  58. Fibrinopeptide A release is necessary for effective B:b interactions during polymerisation of variant fibrinogens with impaired A:a interactions. PMID: 23238100
  59. d-dimer and fibrinogen concentrations were significantly and positively, although weakly, associated with measures related to pulse wave analysis. PMID: 23020230
  60. Data indicate that PDGF enhances the adhesion of CD44v-coated beads to immobilized fibrin(ogen). PMID: 23056168
  61. Patients with heritable dysfibrinogenaemia and harbouring a candidate single nucleotide variations within highly restricted regions of the FGN genes had similar laboratory features irrespective of genotype. PMID: 23061815
  62. Regulation of the fibrinogen genes from proximal promoters and enhancers, the influence of acute-phase stimulation, post-transcriptional regulation by miRNAs and functional regulatory variants identified in genetic studies. Review. PMID: 22836683
  63. Fibrinogen Gdansk: hypofibrinogenemia associated with a novel missense mutation in FGA (Ser112Pro). PMID: 22795623
  64. Impaired lung function is a correlate of fibrinogen levels and the presence of higher fibrinogen levels increases the risk of mortality both in the overall population and among subjects with COPD. PMID: 22489912
  65. Streptococcus gordonii FSS2 Challisin affects fibrin clot formation by digestion of the alphaC region and cleavage of the N -terminal region of the Bbeta chains of fibrinogen PMID: 22552295
  66. These findings thereby define an adaptor-specific mechanism in the control of fibrinogen uptake and implicate that DAB2 is the key adaptor in the clathrin-associated endocytic complexes to mediate fibrinogen internalization. PMID: 22705885
  67. Molecular analyses of both genetic variants suggest that the lack of a mature Aalpha-chain, impaired assembly, and/or secretion of the fibrinogen molecule may lead to afibrinogenemia. PMID: 22639050
  68. These results suggest that immunoreactivity of Fg in the kidney, as well as urinary excretion of Fg, serves as a sensitive and early diagnostic translational biomarker for detection of acute kidney injury. PMID: 22819533
  69. The dynamics of bovine serum albumin (BSA) and human fibrinogen (Fg) at low concentrations were observed at the solid-aqueous interface as a function of temperature. PMID: 22713578
  70. analysis of the differential effect of soluble fibrinogen as a neutrophil activator PMID: 22342352
  71. we observed association of ischemic stroke with allele/genotype combinations of genes IL6, FGA and FGB, in which IL6 plays key role and FGA and FGB have modulating function. PMID: 22642105
  72. A patient had 2 heterozygous nonsense mutation, AaGly13Glu (found also in 2 relatives) and a novel mutation Aa Ser314Cys which seems to affect fibrin morphology PMID: 22407772
  73. a model of fibrin alphaC polymer structure and molecular mechanism of assembly PMID: 22397628
  74. The serum level of fibrinogen Aalpha chain fragment tended toward negative correlation with plasmin-alpha2-plasmin inhibitor complex (P=0.073) and had a positive correlation with thrombin-anti-thrombin complex (P=0.031). PMID: 21891970
  75. genetic polymorphism is associated with resistance to fibrinolysis with a gene-dose effect PMID: 21800001
  76. analysis of fibrinogen and cognitive performance in three Scottish cohorts PMID: 21258858
  77. The reported variants confirm that the AalphaCys36-BbetaCys65 and AalphaCys45-gammaCys23 disulfide bonds are critical for fibrinogen assembly and/or secretion. PMID: 21459789
  78. Hybrid human-chicken fibrinogen containing homologous chicken fibrinogen alpha chain can assemble into polymers that are similar to protofibrils, but these polymers are unable to assemble into fibrin fibers. PMID: 21932842
  79. Study of cross-linked Aalpha221-610 oligomers clarifies the structure of the fibrinogen alphaC-domains in fibrin alphaC polymers and confirms the hypothesis that their binding sites are exposed upon polymerization PMID: 21806028
  80. those with fibrinogen levels around 1 g/l (n = 7) were found to be heterozygous for a novel frameshift mutation in FGA exon 5 (c.1846 del A) and those with undetectable fibrinogen levels (n = 5) were homozygous for the same mutation. PMID: 21245743
  81. The sialic acids exposed on the erythrocyte membrane contribute for the interaction with fibrinogen. PMID: 21464904
  82. Homozygous c.934_935insA in FGA is a cause of inherited afibrinogenemia. PMID: 19698251
  83. Changes during pregnancy in plasma D-dimer, protein S and fibrinogen were confirmed. PMID: 21190511
  84. The molecular weight of the Aalpha fibrinogen chain was consistently higher by up to 1500 Da in neonates and children compared to adults, providing a possible explanation of the thromboprotective mechanism that is functioning in neonates and children. PMID: 21192254
  85. At the beginning of polymerization the major structures were monomers; at the middle of the lag period there were monomers, oligomers, protofibrils and fibers; At the end, there were primarily monomers and fibers, giving way to mainly fibers PMID: 21248064
  86. These data provide important quantitative and qualitative characteristics of alphaIIbbeta3-fibrinogen binding and unbinding that underlie the dynamics of platelet adhesion and aggregation in blood flow. PMID: 21190668
  87. Describe the characterization of a novel FGA intron 2 donor splice-site mutation (Fibrinogen Montpellier II) identified in three siblings with hypodysfibrinogenaemia. PMID: 20806111
  88. D-dimer levels are frequently raised during an acute painful crisis. PMID: 21063468
  89. Two cases of asymptomatic dysfibrinogenemias, found by routine coagulation testing, were genetically identified as new cases of fibrinogen variants Aalpha Arg16His and Aalpha Arg16Cys. PMID: 20829681
  90. Data suggest that the expression of FGA was regulated by miR-759 through its interaction at the polymorphic 3'UTR sequence, which was associated with the susceptibility to CTEPH. PMID: 20677013
  91. FXIII activation peptide segment derives most of its substrate specificity from the P(9)-P(1) segment; kinetic effects of introducing fibrinogen Aalpha character into the FXIII AP segment PMID: 20218626
  92. Comparison of clots of fibrinogen fractions lacking approximately 90% of their alpha chain carboxyl terminal regions, n = 2, displayed faster plasmin-induced lysis than corresponding controls. PMID: 20019599
  93. Two fragments were under-expressed in diabetic patients, while Fibrinopeptide A was over-expressed, suggesting that anomalous turnover of Fibrinopeptide A could be involved in the pathogenesis of diabetic nephropathy. PMID: 19631771
  94. The study reports the identification and functional analysis of a novel nonsense mutation in FGA exon 5: c.718C>T (CAG>TAG) p.Q240X (Q221X in the mature chain lacking the signal peptide), accounting for fibrinogen deficiency in six Egyptian patients. PMID: 20051841
  95. Report fibrinogen Aalpha chain composition in blood. PMID: 20217997
  96. The frequency of hemostatic clinical & laboratory anomalies at presentation of probands from unrelated families with fibrinogen structural defects due to mutations Aalpha R16C or Aalpha R16S was determined. PMID: 19923982
  97. Although IL-6 responsive binding sites are present in fibrinogen gene promoter regions, we did not find strong evidence of interaction between fibrinogen SNPs and IL6 SNPs or levels influencing CVD. PMID: 20059469
  98. Full-length bovine alphaC-domain, bAalpha374-568, and its human counterpart, hAalpha392-610, can form ordered oligomers in a similar manner. PMID: 19928926
  99. genotypes are associated with plasma fibrinogen levels in Chinese PMID: 11546832
  100. The TaqI polymorphism is due to a 28bp duplication at 6587-6614. PMID: 11583334

Show More

Hide All

Involvement in disease Congenital afibrinogenemia (CAFBN); Amyloidosis 8 (AMYL8); Dysfibrinogenemia, congenital (DYSFIBRIN)
Subcellular Location Secreted
Tissue Specificity Detected in blood plasma (at protein level).
Database Links

HGNC: 3661

OMIM: 105200

KEGG: hsa:2243

STRING: 9606.ENSP00000306361

UniGene: Hs.351593

Most popular with customers

Newsletters

Get all the latest information on Events, Sales and Offers. Sign up for newsletter today.

© 2007-2020 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1