Human Ghrelin(GHRL) ELISA Kit

Code CSB-E13398h
Size 96T,5×96T,10×96T How to order?
Trial Size 24T ELISA kits trial application
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Product Details

Description

This Human GHRL ELISA Kit was designed for the quantitative measurement of Human GHRL protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.625 pg/mL-40 pg/mL and the sensitivity is 0.156 pg/mL.

Target Name ghrelin/obestatin prepropeptide
Alternative Names Appetite regulating hormone ELISA Kit; Ghrelin 27 ELISA Kit; Ghrelin 28 ELISA Kit; Ghrelin and obestatin prepropeptide ELISA Kit; Ghrelin ELISA Kit; Ghrelin/obestatin prepropeptide ELISA Kit; ghrl ELISA Kit; GHRL_HUMAN ELISA Kit; Growth hormone releasing peptide ELISA Kit; Growth hormone secretagogue ELISA Kit; Growth hormone-releasing peptide ELISA Kit; M46 protein ELISA Kit; Motilin related peptide ELISA Kit; Motilin-related peptide ELISA Kit; MTLRP ELISA Kit; Obestatin ELISA Kit; Protein M46 ELISA Kit
Abbreviation GHRL
Uniprot No. Q9UBU3
Species Homo sapiens (Human)
Sample Types serum, plasma, cell culture supernates, tissue homogenates
Detection Range 0.625 pg/mL-40 pg/mL
Sensitivity 0.156 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human GHRL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:100 Average % 86  
Range % 80-92  
1:200 Average % 99  
Range % 91-105  
1:400 Average % 101  
Range % 92-110  
1:800 Average % 93  
Range % 86-98  
Recovery
The recovery of human GHRL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 93 89-98  
EDTA plasma (n=4) 94 90-100  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
40 2.921 2.903 2.912 2.826  
20 2.405 2.348 2.383 2.297  
10 1.663 1.643 1.653 1.567  
5 1.053 0.989 1.021 0.935  
2.5 0.575 0.603 0.589 0.503  
1.25 0.322 0.319 0.321 0.235  
0.625 0.214 0.229 0.222 0.136  
0 0.089 0.083 0.086    
Materials provided
  • A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human GHRL antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
  • Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
  • One vial Biotin-labeled GHRL antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
  • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
  • One vial Biotin-antibodyDiluent (15 ml/bottle) ---Dilute the Biotin-antibody.
  • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
  • One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
  • One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
  • One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
  • One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
  • Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
  • An instruction manual
Materials not provided
  • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
  • An incubator can provide stable incubation conditions up to 37°C±5°C.
  • Centrifuge
  • Vortex
  • Squirt bottle, manifold dispenser, or automated microplate washer
  • Absorbent paper for blotting the microtiter plate
  • 50-300ul multi-channel micropipette
  • Pipette tips
  • Single-channel micropipette with different ranges
  • 100ml and 500ml graduated cylinders
  • Deionized or distilled water
  • Timer
  • Test tubes for dilution
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Citations

Customer Reviews and Q&A

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 Q&A
Q:

I need Test Elisa for Human Acylated Ghrelin Test ....do you can help me...what is it?
and do you have this product?

A:
Thanks for your inquiry.
Yes,we have the requested kit available:
#CSB-E13398h,Human Ghrelin(GHRL) ELISA Kit.It is for acylated Ghrelin. Pls let me know if you have any further questions. Thank you.

Target Background

Function
(From Uniprot)
Ghrelin is the ligand for growth hormone secretagogue receptor type 1 (GHSR). Induces the release of growth hormone from the pituitary. Has an appetite-stimulating effect, induces adiposity and stimulates gastric acid secretion. Involved in growth regulation.; Obestatin may be the ligand for GPR39. May have an appetite-reducing effect resulting in decreased food intake. May reduce gastric emptying activity and jejunal motility.
Gene References into Functions
  1. In this review we highlight the multifaceted nature of ghrelin and summarize its glucoregulatory action and discuss the pharmacological value of ghrelin pathway inhibition for the treatment of glucose intolerance and type 2 diabetes. PMID: 29412824
  2. This review examines the biochemical properties of the obestatin peptide (its structure, sequence, stability and distribution) and the candidate receptors through which it may act. It provides a balanced examination of the reported pancreatic and extrapancreatic actions of obestatin and evaluates its potential relevance with respect to diabetes therapy PMID: 29412827
  3. Expression of ghrelin and GHSR1a was studied in human pituitary adenomas. PMID: 28377557
  4. co-administration of Ghr and GH is a promising therapeutic tool for reversing immunosuppression caused by sepsis in the geriatric population. PMID: 28115288
  5. The aim of this study was to determine serum ghrelin and leptin levels in obese and lean Saudi women with Polycystic ovary syndrome and to investigate their relationship to the metabolic profiles in these women. PMID: 30131073
  6. A better understanding of ghrelin in depression could potentially help to optimize future therapeutic --{REVIEW} PMID: 29395244
  7. After Roux-en-Y Gastric bypass, over expression of Ghrelin gene occurs only in the excluded stomach with no correlation to T2 diabetes remission. PMID: 29307107
  8. our data indicate that the splice variant In1-ghrelin could play relevant functional roles in the regulation of breast cancer development and progression PMID: 29272342
  9. down-regulation of ghrelin receptors in substantia nigra pars compacta-dopaminergic (DA) neurons induced the initial dysfunction of DA neurons, leading to extrapyramidal disorder under Parkinson's disease. PMID: 29458391
  10. Data suggest that expression of mRNA for ghrelin and VEGFA are up-regulated in endometrium of women with recurrent miscarriage; thus, ghrelin and VEGFA may play roles in pathogenesis of recurrent miscarriage. These case-control studies were conducted with endometrial tissue obtained during secretory phase of menstrual cycle. (VEGFA = vascular endothelial growth factor A) PMID: 29221937
  11. Serum level of obestatin was significantly lower in the non-diabetic obese patients.Serum obestatin level strongly correlates with lipoprotein subfractions in non-diabetic obese patients. PMID: 29506551
  12. H. pylori infection and severity of gastric corpus pathology are associated with lower serum ghrelin. PMID: 29391762
  13. Data confirm that cord blood levels of ghrelin, leptin, and insulin of term newborns correlate with anthropometric parameters at birth (birth weight, head circumference, etc.). PMID: 29320365
  14. Concentrations of ghrelin were significantly lower in breast milk than in blood plasma from women that carried to term or gave birth prematurely. PMID: 29375043
  15. Individuals with higher levels of fasting ghrelin are more sensitive to reward, but less sensitive to PMID: 29136100
  16. low Plasma ghrelin expression is associated with metabolic syndrome. PMID: 28539201
  17. H. pylori infection decreased serum concentrations of leptin and obestatin in Mexican schoolchildren, suggesting alterations in regulating appetite and energy homeostasis. PMID: 28422951
  18. findings indicate that neither -604G/A and nor -501A/C polymorphisms of ghrelin gene are associated with PCOS, but suggest a relation between the presence of polymorphic allele of -501A/C polymorphism and LDL-C level in a sample of Iranian women. PMID: 29230615
  19. he 72Met allele of the preproghrelin Leu72Met polymorphism may be associated with rehabilitation of depression in male Chinese Han adolescents after the natural disaster. PMID: 28570394
  20. ghrelin effectively suppressed TNF-alpha-induced inflammatory factors' (including ICAM-1, VCAM-1, MCP-1, and IL-1beta) expression through inhibiting AMPK phosphorylation and p65 expression both in HUVEC and THP-1. PMID: 28653238
  21. The results showed that pretreatment with ghrelin reduced H2O2-induced cellular apoptosis and ROS accumulation, increased the expression levels of SOD and CAT, and decreased the expression level of MDA. PMID: 29129986
  22. Medium chain triglyceride nutritional supplementation increased the amount of activated ghrelin and NPY in anorexia nervosa patients. PMID: 28361739
  23. The establishment of the exact correlation between ghrelin, appetite and obesity could be vital for the fight against obesity. PMID: 29102924
  24. Studied expression of In1-ghrelin splice variant in prostate cancer and its possible role in tumorigenesis and disease progression. PMID: 28851363
  25. These results indicate that ghrelin promotes A549cell proliferation via GHSR-dependent PI3K/Akt/mTOR/P70S6K and ERK signaling pathways. PMID: 29524402
  26. findings are the first to associate methylation levels in blood with brain activity in obesity-related regions, and further support previous findings between ghrelin, brain activity and genetic differences PMID: 28194012
  27. The results of this study showed that higher serum levels of obestatin were associated with macro albuminuria suggesting that obestatin may have a role in underlying pathogenic mechanisms that leads to diabetic nephropathy. PMID: 28091434
  28. The level of serum ghrelin might be a biomarker of executive function and become a strong predictor of executive function impairment in patients with type 2 diabetes mellitus. PMID: 27689345
  29. Study found three gene variants (CLOCK-rs4864548, PEMT-rs936108, and GHRELIN-rs696217) that exhibited uncorrected gene-by-sleep duration interactions in relation to BMI z-scores in a cohort of New Zealand European children. However, no interactions were identified in percentage body fat differences. Notably, these interactions are evident without detectable effects on sleep duration. PMID: 28899534
  30. In short, thin children, despite elevated ghrelin production, the low IGF-I concentration is observed, probably due to undernutrition and worse IGF-I formation. In short, normal-weight children and in short, obese ones, ghrelin and IGF-I production is normal, and it seems that mechanisms responsible for their short stature are other than low IGF-I. PMID: 27557428
  31. findings suggest that the Leu72Leu genotype may lead to increased risk of Alcohol Use Disorder possibly via mechanisms involving a lower response to alcohol resulting in excessive alcohol consumption. PMID: 28481975
  32. Ghrelin is involved in appetite-regulating pathways during depression. PMID: 24671339
  33. Data suggest that subjects with anorexia nervosa display broad spectrum of physical activity (2479-26,047 steps/day) which shows a negative correlation with plasma kisspeptin levels and a positive association with plasma ghrelin levels. PMID: 27693487
  34. ghrelin was able to activate the proteasome in neural cells playing also a role in the interplay between the ubiquitin-proteasome system and autophagy. PMID: 26033219
  35. The G/G genotype of the A-604G SNP in the GHRL gene is associated with altered serum ghrelin levels and obesity. PMID: 28597412
  36. Ghrelin SNPs rs26802, rs10490816, and rs696217 were not associated with risk for metabolic syndrome in Chinese Han population. PMID: 28164505
  37. Carrying a G to T substitution in rs696217 preproghrelin gene seems to mark a successful weight loss outcome after gastric bypass in morbidly obese patients PMID: 27681093
  38. Low plasma ghrelin level is associated with mild cognitive impairment in type 2 diabetic patients. PMID: 28146431
  39. There might be a strong correlation between FGF-23 and ghrelin levels irrespective of the stage of chronic kidney disease and the dialysis modality PMID: 26125281
  40. Acylated ghrelin increases in obese individuals pre- and 30, 60, 90 and 120 minutes post prandial. PMID: 28625043
  41. Hemodialysis improves upper GI symptoms and gastric slow waves in CKD patients. An increase in ghrelin and a decrease in GLP-1 might be involved in the HD-induced improvement in gastric slow waves. PMID: 28566304
  42. In the obese polycystic ovary syndrome group, anti-mullerian hormone was associated with ghrelin levels independent of age, insulin, and total testosterone. There was no association between total ghrelin and anti-mullerian hormone levels in non-obese women with polycystic ovary syndrome, non-obese controls, or obese controls. PMID: 28004236
  43. Circulating obestatin levels were not different between lean and obese participants. PMID: 28174289
  44. Studies suggest that metformin treatment was not associated with a decrease in blood leptin levels, or increases in blood ghrelin levels in patients with type 2 diabetes mellitus (T2DM). PMID: 27380451
  45. Ghrelin was significantly higher and the obestatin/ghrelin ratio was significantly lower in celiac disease (CD) patients compared with both diarrhea-predominant irritable bowel syndrome (IBS-d) and healthy controls (HC). Significant differences were found in the Leu72Met polymorphism among groups, with the reduction of the GT genotype and the T allele in both CD and IBS-d patients compared with HC. PMID: 27750262
  46. Synovial fluid ghrelin levels demonstrated an independent and negative association with meniscus injury, cartilage damage, and clinical severity in patients with anterior cruciate ligament deficiency. PMID: 28147215
  47. Based on the obtained results, it was proposed that ghrelin may be considered as playing a role in the etiopathogenesis of hyperemesis gravidarum that may result in disruption of the relationship between nesfatin-1 and ghrelin. PMID: 27235705
  48. In hemodialysis patients, plasma ghrelin level was associated with the endoscopic and serological severity of atrophy related to H. pylori infection. PMID: 28058025
  49. studies indicate that pharmacological targeting of the endogenous ghrelin system is a valuable approach to treat metabolic complications. PMID: 28398233
  50. Patients with intestinal metaplasia, a known precursor of gastric cancer, had significantly less plasma ghrelin levels and ghrelin/obestatin (G/O) ratio than those without intestinal metaplasia. PMID: 28419119

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Subcellular Location Secreted.
Protein Families Motilin family
Tissue Specificity Highest level in stomach. All forms are found in serum as well. Other tissues compensate for the loss of ghrelin synthesis in the stomach following gastrectomy.
Database Links

HGNC: 18129

OMIM: 605353

KEGG: hsa:51738

STRING: 9606.ENSP00000335074

UniGene: Hs.590080

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