The Human Glycated hemoglobin A1c (GHbA1c) ELISA Kit is suitable for the quantitative measurement of GHbA1c in lysates for RBC (red blood cell). This kit is easy to operate and can help to streamline the experimental procedures. It has undergone rigorous quality control in multiple parameters, including specificity, sensitivity, precision, linearity, and lot-to-lot consistency.
The detection mechanism is based on the direct competitive inhibition enzyme immunoassay technique. The microplate has been pre-coated antibody specific for GHbA1c. Standards and samples are pipetted into the wells with Biotin-conjugated GHbA1c. A competitive inhibition reaction is launched between GHbA1c (Standards or samples) and Biotin-conjugated GHbA1c with the pre-coated GHbA1c antibody. After washing, HRP-avidin is added to the wells. Following a wash to remove any unbound reagent, the substrate solution is added to the wells and color develops in opposite to the amount of GHbA1c bound in the initial step. The color development is stopped and the intensity of the color is measured.
GHbA1c is a sugar-bound hemoglobin that is formed through the non-enzymatic binding of circulating glucose to hemoglobin. This process is known as glycation. The rate of GHbA1c formation is positively correlated with the surrounding blood glucose concentration. GHbA1c is a remarkable indicator for Diabetes Mellitus (DM) patients and can evaluate long-term average glycemia.