Human Glycated hemoglobin A1c,GHbA1c ELISA Kit

Code CSB-E08139h
Size 96T,5×96T,10×96T How to order?
Trial Size 24T ELISA kits trial application
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Product Details

Description

This Human GHbA1c ELISA Kit was designed for the quantitative measurement of Human GHbA1c protein in lysates for RBC. It is a Competitive ELISA kit, its detection range is 0.012 ng/mL-50 ng/mL and the sensitivity is 0.012 ng/mL.

Target Name Glycated hemoglobin A1c,GHbA1c
Abbreviation GHbA1c
Species Homo sapiens (Human)
Sample Types lysates for RBC
Detection Range 0.012 ng/mL-50 ng/mL
Sensitivity 0.012 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Others
Assay Principle quantitative
Measurement Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average    
50 0.270 0.273 0.272    
12.5 0.367 0.378 0.373    
3.125 0.463 0.489 0.476    
0.78 0.560 0.598 0.579    
0.195 0.692 0.672 0.682    
0.049 1.384 1.368 1.376    
0.012 1.994 1.954 1.974    
0 2.373 2.399 2.386    
Materials provided
  • A 96-well Assay plate --The 96-well plate has been pre-coated with GHbA1c antibody.
  • Standard (10 x concentrate) (1 x 200 μl) --Dilute the standard at dilution series, read the OD values, and then draw a standard curve.
  • Biotin-conjugated GHbA1c (100 x concentrate) (1 x 60 μl)
  • HRP-avidin (100 x concentrate) (1 x 120 μl) --Catalyze the TMB solution, eliciting a chromogemic reaction.
  • Biotin-conjugate Diluent (1 x 10 ml) --Dilute the Biotin-conjugated GHbA1c
  • HRP-avidin Diluent (1 x 20 ml) --Dilute the HRP-avidin
  • Sample Diluent (2 x 20 ml) --Reconstitute the standard and dilute the sample to an appropriate concentration.
  • Wash Buffer (25x concentrate) (1 x 20 ml)--Wash away unbound or free substances.
  • TMB Substrate (1x 10 ml) --Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
  • Stop Solution (1 x 10ml) --Stop the color reaction. The solution color immediately turns from blue to yellow.
  • Four Adhesive Strips (For 96 wells)
  • An Instruction manual
Materials not provided
  • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm - 570 nm.
  • An incubator that can provide stable incubation conditions up to 37°C±5°C.
  • Centrifuge
  • Vortex
  • Squirt bottle, manifold dispenser, or automated microplate washer
  • Absorbent paper for blotting the microtiter plate
  • 50-300ul multi-channel micropipette
  • Pipette tips
  • Single-channel micropipette with different ranges
  • 100ml and 500ml graduated cylinders
  • Deionized or distilled water
  • Timer
  • Test tubes for dilution
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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