Mouse Glycated hemoglobin A1c,GHbA1c ELISA Kit

Code CSB-E08141m
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Lead Time 3-5 working days
Abbreviation GHbA1c
Target Name Glycated hemoglobin A1c,GHbA1c
Species Mus musculus (Mouse)
Sample Types lysates for RBC
Detection Range 0.78 μg/mL-50 μg/mL
Sensitivity 0.39 μg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Others
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Recovery
The recovery of mouse GHbA1c spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
lysate for RBC (n=5) 95 90-99  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
μg/ml OD1 OD2 Average    
50 0.175 0.174 0.175    
25 0.272 0.269 0.271    
12.5 0.465 0.506 0.486    
6.25 0.736 0.739 0.738    
3.12 1.424 1.411 1.418    
1.56 2.038 2.069 2.054    
0.78 2.541 2.529 2.535    
0 2.715 2.797 2.756    
References
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