Human High mobility group protein B1,HMGB-1 ELISA Kit

Code CSB-E08223h
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P09429
Lead Time 3-5 working days
Abbreviation HMGB1
Target Name high-mobility group box 1
Alias DKFZp686A04236, HMG1, HMG3, SBP-1, Amphoterin|OTTHUMP00000018196|OTTHUMP00000190860|OTTHUMP00000200117|Sulfoglucuronyl carbohydrate binding protein|high mobility group box 1|high mobility group prot
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 78 pg/mL-5000 pg/mL
Sensitivity 19.5 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Epigenetics and Nuclear Signaling
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
HGNC 4983
RGD 2802
MGI 96113
Precision
Intra-assay Precision (Precision within an assay): CV%<8%        
Three samples of known concentration were tested twenty times on one plate to assess.    
Inter-assay Precision (Precision between assays): CV%<10%        
Three samples of known concentration were tested in twenty assays to assess.      
               
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human HMGB-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
  Sample Serum(n=4)    
1:1 Average % 90    
Range % 84-99    
1:2 Average % 88    
Range % 82-94    
1:4 Average % 95    
Range % 89-102    
1:8 Average % 94    
Range % 88-100    
Recovery
The recovery of human HMGB-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
 
Sample Type Average % Recovery Range    
Serum (n=5) 98 90-102    
EDTA plasma (n=4) 91 85-100    
               
               
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
 
pg/ml OD1 OD2 Average Corrected    
5000 1.912 1.831 1.872 1.780    
2500 1.454 1.369 1.412 1.320    
1250 0.839 0.789 0.814 0.722    
625 0.456 0.487 0.472 0.380    
312 0.283 0.297 0.290 0.198    
156 0.199 0.202 0.201 0.109    
78 0.103 0.108 0.106 0.014    
0 0.089 0.094 0.092      
References
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Function Multifunctional redox sensitive protein with various roles in different cellular compartments. In the nucleus is one of the major chromatin-associated non-histone proteins and acts as a DNA chaperone involved in replication, transcription, chromatin remodeling, V(D)J recombination, DNA repair and genome stability. Proposed to be an universal biosensor for nucleic acids. Promotes host inflammatory response to sterile and infectious signals and is involved in the coordination and integration of innate and adaptive immune responses. In the cytoplasm functions as sensor and/or chaperone for immunogenic nucleic acids implicating the activation of TLR9-mediated immune responses, and mediates autophagy. Acts as danger associated molecular pattern (DAMP) molecule that amplifies immune responses during tissue injury
Subcellular Location Nucleus, Chromosome, Cytoplasm, Secreted, Cell membrane, Peripheral membrane protein, Extracellular side, Endosome, Endoplasmic reticulum-Golgi intermediate compartment
Protein Families HMGB family
Tissue Specificity Ubiquituous. Expressed in platelets (PubMed:11154118).
Database Links

HGNC: 4983

OMIM: 163905

KEGG: hsa:3146

STRING: 9606.ENSP00000343040

UniGene: Hs.434102

Pathway Autophagy
Necroptosis
DNA repair pathway

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