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Human Hyaluronic acid,HA ELISA Kit

Instructions
Code CSB-E04805h
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name Hyaluronic acid,HA
Alternative Names N/A
Abbreviation HA
Species Homo sapiens (Human)
Sample Types serum, plasma, cerebrospinal fluid (CSF), tissue homogenates
Detection Range 0.156 ng/mL-10 ng/mL
Sensitivity 0.039 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cell Biology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human HA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
　 Sample Serum(n=4)  
1:20 Average % 87  
Range % 80-94  
1:40 Average % 92  
Range % 88-99  
1:80 Average % 97  
Range % 91-100  
1:160 Average % 101  
Range % 97-105  
Recovery
The recovery of human HA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 100 95-105  
EDTA plasma (n=4) 93 88-98  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
10 2.727 2.700 2.714 2.626  
5 2.231 2.345 2.288 2.200  
2.5 1.558 1.655 1.607 1.519  
1.25 0.831 0.901 0.866 0.778  
0.625 0.477 0.451 0.464 0.376  
0.312 0.301 0.322 0.312 0.224  
0.156 0.189 0.174 0.182 0.094  
0 0.087 0.089 0.088 　  
Troubleshooting
and FAQs		 ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Citations

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Q&A and Customer Reviews

■ Q&A
Q:

We are looking into the best method to distinguish HA manufactured by fermentation and nature human HA.

A:
Thanks for your inquiry.
CSB-E04805h Human Hyaluronic acid, HA ELISA Kit
This kit can detect nature HA in polymerized form. As for HA manufactured by fermentation, it usually exists in free form.
We think it can not be detected out by the kit.
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