Mouse Hyaluronic acid,HA ELISA Kit

Code CSB-E08121m
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Description

This Mouse Hyaluronic acid (HA) ELISA Kit was designed for the quantitative measurement of Mouse Hyaluronic acid (HA) protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 62.5 pg/mL-4000 pg/mL and the sensitivity is 15.6 pg/mL .

Target Name Hyaluronic acid,HA
Alternative Names N/A
Abbreviation HA
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 62.5 pg/mL-4000 pg/mL
Sensitivity 15.6 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cell Biology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse HA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:100 Average % 89  
Range % 82-99  
1:200 Average % 93  
Range % 89-101  
1:400 Average % 102  
Range % 98-106  
1:800 Average % 97  
Range % 92-102  
Recovery
The recovery of mouse HA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 90 87-94  
EDTA plasma (n=4) 97 93-107  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
4000 2.755 2.856 2.806 2.652  
2000 2.027 2.128 2.078 1.924  
1000 1.435 1.455 1.445 1.291  
500 0.850 0.861 0.856 0.702  
250 0.526 0.515 0.521 0.367  
125 0.358 0.348 0.353 0.199  
62.5 0.267 0.261 0.264 0.110  
0 0.154 0.153 0.154    
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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