Human Inter-alpha-trypsin inhibitor heavy chain H2(ITIH2) ELISA kit

Code CSB-EL011895HU
Size 96T,5×96T,10×96T
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Product Details

Target Name
inter-alpha (globulin) inhibitor H2
Alternative Names
AI747202 ELISA Kit; H2P ELISA Kit; IGHEP2 ELISA Kit; Inter alpha (globulin) inhibitor H2 ELISA Kit; Inter alpha globulin inhibitor H2 polypeptide ELISA Kit; Inter alpha trypsin inhibitor complex component II ELISA Kit; Inter alpha trypsin inhibitor heavy chain H2 ELISA Kit; Inter-alpha-inhibitor heavy chain 2 ELISA Kit; Inter-alpha-trypsin inhibitor complex component II ELISA Kit; Inter-alpha-trypsin inhibitor heavy chain H2 ELISA Kit; Intin2 ELISA Kit; ITI HC2 ELISA Kit; ITI heavy chain H2 ELISA Kit; ITI-HC2 ELISA Kit; Itih 2 ELISA Kit; Itih2 ELISA Kit; ITIH2_HUMAN ELISA Kit; OTTHUMP00000019058 ELISA Kit; OTTHUMP00000019059 ELISA Kit; OTTMUSP00000012019 ELISA Kit; RP23 119N4.3 ELISA Kit; Serum derived hyaluronan associated protein ELISA Kit; Serum-derived hyaluronan-associated protein ELISA Kit; SHAP ELISA Kit
Abbreviation
ITIH2
Uniprot No.
Species
Homo sapiens (Human)
Sample Types
serum, plasma, tissue homogenates
Detection Range
3.12 μg/mL-200 μg/mL
Sensitivity
0.78 μg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Cell Biology
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human ITIH2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:5Average %91
Range %86-94
1:10Average %97
Range %90-104
1:20Average %101
Range %96-107
1:40Average %89
Range %84-94
Recovery
The recovery of human ITIH2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9088-95
EDTA plasma (n=4)10297-107
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
μg/mlOD1OD2AverageCorrected
2002.439 2.555 2.497 2.312
1001.867 1.728 1.798 1.613
501.221 1.265 1.243 1.058
250.751 0.757 0.754 0.569
12.50.412 0.422 0.417 0.232
6.250.318 0.336 0.327 0.142
3.120.272 0.281 0.277 0.092
00.183 0.186 0.185
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Human ITIH2 ELISA Kit was designed for the quantitative measurement of Human ITIH2 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 3.12 μg/mL-200 μg/mL and the sensitivity is 0.78 μg/mL.

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Target Background

Function
(From Uniprot)
May act as a carrier of hyaluronan in serum or as a binding protein between hyaluronan and other matrix protein, including those on cell surfaces in tissues to regulate the localization, synthesis and degradation of hyaluronan which are essential to cells undergoing biological processes.
Gene References into Functions
  1. It forms complex with hyaluronan and the comlex is a functional molecule involved in inflammation. PMID: 20297716
  2. TSG-6 acts as cofactor and catalyst in the production of IalphaI heavy chain x hyaluronan complexes PMID: 15840581
  3. Leukocytes infiltrated to the synovium were strongly positive for hyaluronic acid, SHAP, and CD44 on their surfaces, suggesting a role for the adhesion-enhancing effect of SHAP in inflammation. PMID: 16702221
  4. Increased levels of SHAP-HA complex in sera are possible predictive markers for cervical ripening in premature labor. PMID: 18382897
  5. serum-derived hyaluronan-associated protein-hyaluronan complex has a role in progression of ovarian cancer PMID: 18425383

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Subcellular Location
Secreted.
Protein Families
ITIH family
Tissue Specificity
Plasma.
Database Links

HGNC: 6167

OMIM: 146640

KEGG: hsa:3698

STRING: 9606.ENSP00000351190

UniGene: Hs.75285

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