Human Inter-alpha-trypsin inhibitor heavy chain H3(ITIH3) ELISA kit

Code CSB-EL011896HU
Size 96T,5×96T,10×96T
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Product Details

Target Name
inter-alpha (globulin) inhibitor H3
Alternative Names
ITIH3 ELISA Kit; Inter-alpha-trypsin inhibitor heavy chain H3 ELISA Kit; ITI heavy chain H3 ELISA Kit; ITI-HC3 ELISA Kit; Inter-alpha-inhibitor heavy chain 3 ELISA Kit; Serum-derived hyaluronan-associated protein ELISA Kit; SHAP ELISA Kit
Abbreviation
ITIH3
Uniprot No.
Species
Homo sapiens (Human)
Sample Types
serum, plasma, tissue homogenates
Detection Range
78.13 ng/mL-5000 ng/mL
Sensitivity
19.53 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Others
Assay Principle
quantitative
Measurement
Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human TIH3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:20Average %92
Range %86-99
1:40Average %97
Range %92-103
1:80Average %86
Range %80-89
1:160Average %90
Range %83-99
Recovery
The recovery of human TIH3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9087-95
EDTA plasma (n=4)9590-100
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2Average
50000.083 0.085 0.084
25000.139 0.147 0.143
12500.223 0.220 0.222
6250.329 0.347 0.338
312.50.572 0.614 0.593
156.30.814 0.849 0.832
78.131.228 1.259 1.244
02.081 2.226 2.154
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Human ITIH3 ELISA Kit was designed for the quantitative measurement of Human ITIH3 protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 78.13 ng/mL-5000 ng/mL and the sensitivity is 19.53 pg/mL .

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Target Background

Function
(From Uniprot)
May act as a carrier of hyaluronan in serum or as a binding protein between hyaluronan and other matrix protein, including those on cell surfaces in tissues to regulate the localization, synthesis and degradation of hyaluronan which are essential to cells undergoing biological processes.
Gene References into Functions
  1. ITIH3 rs2535629 SNP was genotyped in N=256 patients receiving various antipsychotics for up to 26weeks. Study found no association of genotype with overall changes in Brief Psychiatric Rating Scale scores, but greater improvement of negative symptoms in minor allele carriers indicates that rs2535629 may help to identify a subset of schizophrenia patients with better treatment response to clozapine. PMID: 27396837
  2. confirmed the association of schizophrenia with ITIH3/4 in a Han Chinese population PMID: 26206863
  3. A novel association between suicide attempt and the ITIH3/4-region in a combined group of patients with bipolar disorder, schizophrenia and related psychosis spectrum disorders. PMID: 24461634
  4. Study showed the association of a polymorphism (rs2535629) of ITIH3 with psychiatric disorders in an Asian population and that rs2535629 influences the susceptibility to psychiatric disorders by affecting the expression level of GLT8D1 PMID: 24373612
  5. This study demonistrated that Genome-wide significant associations in schizophrenia to ITIH3/4 and extensive replication of associations reported by the Schizophrenia. PMID: 22614287
  6. ITIH3 protein was found to be more highly expressed in plasma of tumor bearing mice compared to control. PMID: 20515073
  7. ITIH3 single-nucleotide polymorphism is a novel genetic risk factor of myocardial infarction. PMID: 17211523
  8. Widespread heavy chain 3 was only present in urinary stone formers PMID: 17898697

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Subcellular Location
Secreted.
Protein Families
ITIH family
Database Links

HGNC: 6168

OMIM: 146650

KEGG: hsa:3699

STRING: 9606.ENSP00000415769

UniGene: Hs.76716

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