Human Inter-alpha-trypsin inhibitor heavy chain H3(ITIH3) ELISA kit

Code CSB-EL011896HU
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. Q06033
Lead Time 7-14 working days
Abbreviation ITIH3
Target Name inter-alpha (globulin) inhibitor H3
Alias H3P, inter-alpha (globulin) inhibitor, H3 polypeptide|inter-alpha-trypsin inhibitor heavy chain H3|pre-alpha (globulin) inhibitor, H3 polypeptide
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 78.13 ng/mL-5000 ng/mL
Sensitivity 19.53 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Others
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Competitive
HGNC 6168
RGD 620633
MGI 96620
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human TIH3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:20Average %92
Range %86-99
1:40Average %97
Range %92-103
1:80Average %86
Range %80-89
1:160Average %90
Range %83-99
Recovery
The recovery of human TIH3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9087-95
EDTA plasma (n=4)9590-100
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2Average
50000.083 0.085 0.084
25000.139 0.147 0.143
12500.223 0.220 0.222
6250.329 0.347 0.338
312.50.572 0.614 0.593
156.30.814 0.849 0.832
78.131.228 1.259 1.244
02.081 2.226 2.154
References
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Function May act as a carrier of hyaluronan in serum or as a binding protein between hyaluronan and other matrix protein, including those on cell surfaces in tissues to regulate the localization, synthesis and degradation of hyaluronan which are essential to cells undergoing biological processes.
Subcellular Location Secreted
Protein Families ITIH family
Database Links

HGNC: 6168

OMIM: 146650

KEGG: hsa:3699

STRING: 9606.ENSP00000415769

UniGene: Hs.76716

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