Human Interleukin-23 subunit alpha(IL-23A) Elisa kit

Code CSB-E17339h
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. Q9NPF7
Lead Time 3-5 working days
Abbreviation IL23A
Protein Biological Process 1 Immunity
Target Name interleukin 23, alpha subunit p19
Alias IL-23, IL-23A, IL23P19, MGC79388, P19, SGRF, JKA3 induced upon T-cell activation|interleukin 23 p19 subunit
Species Homo sapiens (Human)
Protein Biological Process 3 Antiviral defense
Sample Types serum, plasma, cell culture supernates, tissue homogenates, cell lysates
Detection Range 62.5 pg/mL-4000 pg/mL
Sensitivity 15.6 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This gene encodes a subunit of the heterodimeric cytokine interleukin 23 (IL23). IL23 is composed of this protein and the p40 subunit of interleukin 12 (IL12B). The receptor of IL23 is formed by the beta 1 subunit of IL12 (IL12RB1) and an IL23 specific subunit, IL23R. Both IL23 and IL12 can activate the transcription activator STAT4, and stimulate the production of interferon-gamma (IFNG). In contrast to IL12, which acts mainly on naive CD4(+) T cells, IL23 preferentially acts on memory CD4(+) T cells.
HGNC 15488
RGD 620873
MGI 1932410
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human IL-23A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 88
Range % 83-97
1:2 Average % 97
Range % 91-104
1:4 Average % 84
Range % 81-88
1:8 Average % 89
Range % 81-96
Recovery
The recovery of human IL-23A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 101 94-101
EDTA plasma (n=4) 107 100-114
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
4000 1.762 1.845 1.804 1.628
2000 1.001 1.022 1.012 0.836
1000 0.669 0.667 0.668 0.492
500 0.407 0.416 0.412 0.236
250 0.297 0.277 0.287 0.111
125 0.253 0.246 0.250 0.074
62.5 0.220 0.216 0.218 0.042
0 0.172 0.179 0.176  
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Function Associates with IL12B to form the IL-23 interleukin, a heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to a heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis.
Subcellular Location Secreted
Protein Families IL-6 superfamily
Tissue Specificity Secreted by activated dendritic and phagocytic cells and keratinocytes. Also expressed by dermal Langerhans cells (at protein level).
Database Links

HGNC: 15488

OMIM: 605580

KEGG: hsa:51561

STRING: 9606.ENSP00000228534

UniGene: Hs.382212

Pathway Jak-STAT signaling pathway
Th17 cell differentiation

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