Human Isodesmosine (IDES) ELISA Kit

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Human Isodesmosine (IDES) ELISA Kit

Name: Human Isodesmosine (IDES) ELISA Kit
Brand: CUSABIO
SKU: CSB-E14052h

Citations (1) Protocol MSDS

Code	CSB-E14052h
Size	96T,5×96T,10×96T
Price	Request a Quote
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Product Details
Citations (1)
Customer Reviews and Q&A

Target Name

Isodesmosine (IDES)

Abbreviation

IDES

Species

Homo sapiens (Human)

Sample Types

serum, plasma, urine, tissue homogenates

Detection Range

47 pg/mL-3000 pg/mL

Sensitivity

11.75 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Others

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human IDES in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	85
	Range %	80-92
1:2	Average %	95
	Range %	90-99
1:4	Average %	99
	Range %	94-103
1:8	Average %	86
	Range %	82-92

Recovery

The recovery of human IDES spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	100	95-105
EDTA plasma (n=4)	99	94-104

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
3000	2.291	2.287	2.289	2.156
1500	1.752	1.757	1.755	1.622
750	1.091	1.114	1.103	0.970
375	0.675	0.698	0.687	0.554
187.5	0.438	0.457	0.448	0.315
93.75	0.297	0.282	0.290	0.157
47	0.225	0.235	0.230	0.097
0	0.133	0.132	0.133

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Description

This Human IDES ELISA Kit was designed for the quantitative measurement of Human IDES protein in serum, plasma, urine, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 47 pg/mL-3000 pg/mL and the sensitivity is 11.75 pg/mL .

Procollagen III N-terminal propeptide and desmosine are released by matrix destruction in pulmonary tuberculosis Seddon J et al,J Infect Dis,2013

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

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