Human L-Carnitine ELISA Kit

Code CSB-E13606h
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
Have Questions? Leave a Message or Start an on-line Chat

Product Details

Target Name L-Carnitine
Abbreviation L-Carnitine
Species Homo sapiens (Human)
Sample Types serum, plasma, urine, tissue homogenates
Detection Range 0.312 ng/mL-20 ng/mL
Sensitivity 0.078 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Biochemicals
Assay Principle quantitative
Measurement Sandwich
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
To assess the linearity of the assay, samples were spiked with high concentrations of human L-Carnitine in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:200 Average % 88  
Range % 80-96  
1:400 Average % 97  
Range % 91-105  
1:800 Average % 99  
Range % 92-110  
1:1600 Average % 100  
Range % 90-110  
The recovery of human L-Carnitine spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 93 82-110  
EDTA plasma (n=4) 97 90-110  
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
20 2.749 2.639 2.694 2.543  
10 2.010 1.976 1.993 1.842  
5 1.303 1.294 1.299 1.148  
2.5 0.889 0.839 0.864 0.713  
1.25 0.561 0.524 0.543 0.392  
0.625 0.371 0.359 0.365 0.214  
0.312 0.256 0.239 0.248 0.097  
0 0.155 0.147 0.151    
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Earn $30 Amazon Card or 20μL/μg CUSABIO Trial Size Antibody. Details of rewards >>


Get all the latest information on Events, Sales and Offers. Sign up for newsletter today.

© 2007-2020 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1