| Description |
This Human L-Carnitine ELISA Kit was designed for the quantitative measurement of Human L-Carnitine protein in serum, plasma, urine, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL. |
| Target Name |
L-Carnitine |
| Abbreviation |
L-Carnitine |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, urine, tissue homogenates |
| Detection Range |
0.312 ng/mL-20 ng/mL |
| Sensitivity |
0.078 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Biochemicals
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| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human L-Carnitine in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:200 |
Average % |
88 |
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| Range % |
80-96 |
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| 1:400 |
Average % |
97 |
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| Range % |
91-105 |
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| 1:800 |
Average % |
99 |
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| Range % |
92-110 |
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| 1:1600 |
Average % |
100 |
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| Range % |
90-110 |
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| Recovery |
| The recovery of human L-Carnitine spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
93 |
82-110 |
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| EDTA plasma (n=4) |
97 |
90-110 |
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| Typical Data |
 These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 20 |
2.749 |
2.639 |
2.694 |
2.543 |
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| 10 |
2.010 |
1.976 |
1.993 |
1.842 |
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| 5 |
1.303 |
1.294 |
1.299 |
1.148 |
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| 2.5 |
0.889 |
0.839 |
0.864 |
0.713 |
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| 1.25 |
0.561 |
0.524 |
0.543 |
0.392 |
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| 0.625 |
0.371 |
0.359 |
0.365 |
0.214 |
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| 0.312 |
0.256 |
0.239 |
0.248 |
0.097 |
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| 0 |
0.155 |
0.147 |
0.151 |
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| ELISA Data Analysis |
Watch ELISA data processing video & download Curve Expert if needed |
Troubleshooting
and FAQs |
ELISA kit FAQs |
| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
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