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Human L-Carnitine ELISA Kit

Citations (4) Protocol MSDS
Code CSB-E13606h
Size 96T,5×96T,10×96T
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Product Details

Target Name
L-Carnitine
Abbreviation
L-Carnitine
Species
Homo sapiens (Human)
Sample Types
serum, plasma, urine, tissue homogenates
Detection Range
0.312 ng/mL-20 ng/mL
Sensitivity
0.078 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Biochemicals
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human L-Carnitine in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
　 Sample Serum(n=4)  
1:200 Average % 88  
Range % 80-96  
1:400 Average % 97  
Range % 91-105  
1:800 Average % 99  
Range % 92-110  
1:1600 Average % 100  
Range % 90-110  
Recovery
The recovery of human L-Carnitine spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 93 82-110  
EDTA plasma (n=4) 97 90-110  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
             
             
             
             
             
             
             
             
             
             
             
             
             
ng/ml OD1 OD2 Average Corrected  
20 2.749 2.639 2.694 2.543  
10 2.010 1.976 1.993 1.842  
5 1.303 1.294 1.299 1.148  
2.5 0.889 0.839 0.864 0.713  
1.25 0.561 0.524 0.543 0.392  
0.625 0.371 0.359 0.365 0.214  
0.312 0.256 0.239 0.248 0.097  
0 0.155 0.147 0.151 　  
             
ELISA Data Analysis
ELISA Standard Curve & Curve Expert software
Troubleshooting
and FAQs
ELISA kit FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Description

This Human L-Carnitine ELISA Kit was designed for the quantitative measurement of Human L-Carnitine protein in serum, plasma, urine, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL.

Citations

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