Human Laminin-5(LN-5)ELISA Kit

Code CSB-E13422h
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. O15230
Lead Time 3-5 working days
Abbreviation LAMA5
Protein Biological Process 1 Cell Adhesion
Target Name laminin, alpha 5
Alias RP11-157P1.6, KIAA1907, laminin alpha 5|laminin alpha-5 chain
Species Homo sapiens (Human)
Protein Biological Process 3 Cell adhesion
Sample Types serum, plasma
Detection Range 12.5 ng/mL-800 ng/mL
Sensitivity 3.12 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Neuroscience
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details Components of the extracellular matrix exert myriad effects on tissues throughout the body. In particular, the laminins, a family of heterotrimeric extracellular glycoproteins, affect tissue development and integrity in such diverse organs as the kidney, lung, skin, and nervous system. It is thought that laminins mediate the attachment, migration, and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. Laminins function as heterotrimeric complexes of alpha, beta, and gamma chains, with each chain type representing a different subfamily of proteins. This protein belongs to the alpha subfamily of laminin chains and is a major component of basement membranes. Two transcript variants encoding different isoforms have been found for this gene, but the full-length nature of one of them has not been determined.
HGNC 6485
RGD 621023
MGI 105382
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of Human LN-5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %88
Range %84-94
1:2Average %98
Range %92-102
1:4Average %95
Range %90-100
1:8Average %102
Range %96-106
Recovery
The recovery of Human LN-5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9488-98
EDTA plasma (n=4)8682-92
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
8002.262 2.368 2.315 2.216
4001.676 1.570 1.623 1.524
2000.987 0.960 0.974 0.875
1000.558 0.547 0.553 0.454
500.312 0.324 0.318 0.219
250.243 0.256 0.250 0.151
12.50.172 0.174 0.173 0.074
00.098 0.099 0.099
References
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Function Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
Subcellular Location Secreted, extracellular space, extracellular matrix, basement membrane
Tissue Specificity Expressed in heart, lung, kidney, skeletal muscle, pancreas, retina and placenta. Little or no expression in brain and liver.
Database Links

HGNC: 6485

OMIM: 601033

KEGG: hsa:3911

STRING: 9606.ENSP00000252999

UniGene: Hs.473256

Pathway PI3K-Akt signaling pathway
Focal adhesion

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