Human Laminin-5(LN-5)ELISA Kit

Instructions
Code CSB-E13422h
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name laminin, alpha 5
Alternative Names KIAA0533 ELISA Kit; KIAA1907 ELISA Kit; LAMA5 ELISA Kit; LAMA5_HUMAN ELISA Kit; Laminin alpha 5 chain ELISA Kit; Laminin subunit alpha 5 ELISA Kit; Laminin subunit alpha-5 ELISA Kit; Laminin; alpha 5 ELISA Kit; Laminin-10 subunit alpha ELISA Kit; Laminin-11 subunit alpha ELISA Kit; Laminin-15 subunit alpha ELISA Kit; RP11-157P1.6 ELISA Kit
Abbreviation LAMA5
Uniprot No. O15230
Species Homo sapiens (Human)
Sample Types serum, plasma
Detection Range 12.5 ng/mL-800 ng/mL
Sensitivity 3.12 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Neuroscience
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of Human LN-5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %88
Range %84-94
1:2Average %98
Range %92-102
1:4Average %95
Range %90-100
1:8Average %102
Range %96-106
Recovery
The recovery of Human LN-5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9488-98
EDTA plasma (n=4)8682-92
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
8002.262 2.368 2.315 2.216
4001.676 1.570 1.623 1.524
2000.987 0.960 0.974 0.875
1000.558 0.547 0.553 0.454
500.312 0.324 0.318 0.219
250.243 0.256 0.250 0.151
12.50.172 0.174 0.173 0.074
00.098 0.099 0.099
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Data

Function Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
Gene References into Functions
  1. The finding of rare LAMA5 variants together with FLT4 and FOXC2 mutations in Milroy disease and lymphedema-distichiasis syndrome suggests that these mutations may be co-responsible for these disorders and most likely interfere with the function of lymphatics. PMID: 29908552
  2. This study shows that specific laminin alpha5 substrates provide a controlled in vitro culture environment for human pluripotent stem cells-derived neurons. PMID: 28926760
  3. study describes the first LAMA5 mutation that causes a novel complex extracellular matrix syndrome PMID: 28735299
  4. data show that the BCAM/LAMA5 system plays a functional role in the metastatic spreading of KRAS-mutant colorectal cancer PMID: 27143691
  5. Taken together, these data suggest that alpha5-containing laminins repress intestinal differentiation in its early stages. PMID: 29198708
  6. We report here a severe defect of neuromuscular transmission in a consanguineous patient with a homozygous variant in the laminin alpha-5 subunit gene (LAMA5). The variant c.8046C>T (p.Arg2659Trp) is rare and has a predicted deleterious effect. The affected individual, who also carries a rare homozygous sequence variant in LAMA1, had muscle weakness, myopia, and facial tics PMID: 28544784
  7. The Laminin-511 consists of alpha5, beta1, and gamma1 chains, of which three laminin globular domains of the alpha5 chain (alpha5/LG1-3) and a Glu residue in the C-terminal tail of chain gamma1 (gamma1-Glu1607) are required for binding to integrins. PMID: 28412362
  8. The association of LAMA5 rs4925386 alleles with both inter-individual differences in height and in longevity suggests that laminins may be among the factors linking stature and cancer susceptibility. PMID: 26968355
  9. disruption of the LAMA5 gene dramatically reduced hPSC self-renewal and increased apoptosis without affecting the expression of pluripotency markers. PMID: 26235893
  10. Gal-1 decreased the expression of collagen genes COL3A1 and COL5A1 but increased the expression of fibronectin and laminin 5. PMID: 24503541
  11. Lutheran (Lu), also known as basal cell adhesion molecule (B-CAM), competes with integrins for binding to laminin alpha5, a subunit of LM-511, a major component of basement membranes. PMID: 24036115
  12. The laminin-5 staining pattern was significantly more often continuous in severe dysplasia/carcinoma-in-situ PMID: 23715200
  13. mechanistic role of laminin-511 in tissue homeostasis PMID: 22666383
  14. Polymorphisms in LN5 were associated with a reduced level of hemoglobin and neutropenia in non-small cell lung cancer. PMID: 21461966
  15. These results indicate specific laminin isoforms and integrins in maintenance of human embryonic stem cells pluripotency in feeder-dependent cultures. PMID: 22099024
  16. PDGF-BB is not superior to laminin as a potential marker of advanced CP. PMID: 21921666
  17. Data indicate that the function-blocking antibody against Lu/B-CAM should be useful for not only investigating cell adhesion to laminin alpha5 but also for developing drugs to inhibit sickle cell vaso-occlusion. PMID: 21858073
  18. Cytoplasmic and basement membrane laminin is important in the pathogenesis and invasion of basal cell carcinoma. PMID: 19615022
  19. investigated the relation between epithelial-mesenchymal transition criteria and laminin-332 expression in a cell culture model of transforming growth factor beta-1 (TGFbeta1)/epithelial growth factor (EGF) long time co-stimulation. PMID: 20819124
  20. Abnormal distribution of laminin alpha1 and laminin alpha5 in glomerular basement membrane is correlated with GBM thickening and splitting in human Alport's syndrome. PMID: 20019771
  21. LAMA5 rs659822 regulates anthropometric and metabolic traits in elderly people PMID: 20951195
  22. Data from experiment with peptide fragments suggest laminin alpha5 (and laminin alpha4) may be part of host defense response and may protect tissues from invading pathogens. PMID: 20433883
  23. Laminin-10/11 and fibronectin differentially prevent apoptosis induced by serum removal via phosphatidylinositol 3-kinase/Akt- and MEK1/ERK-dependent pathways (Laminin 10; separate entry for Laminin 11). PMID: 11891225
  24. LN5 has a role in the differentiation of the tracheal epithelium in human embryos PMID: 12242717
  25. LN6 and LN5 have distinct biological activities, but they may cooperatively support cell adhesion PMID: 12379663
  26. role for laminin-5 during human embryogenesis, for example, for epithelial cell development, beyond its involvement in hemidesmosome formation and cell adhesion. PMID: 12382139
  27. Laminins with alpha1, alph4, and alpha5 chains are compared to determine laminin isoform-specific promotion of adhesion and migration of human bone marrow progenitor cells. PMID: 12393739
  28. Our results elucidate a mechanism whereby mesangial cells organize the glomerular capillaries by adhering to the G domain of laminin alpha5 in the glomerular basement membrane. PMID: 12682087
  29. Data suggest that laminin-10 (alpha5beta1gamma1) is required for hair follicle development and report the first use of exogenous protein to correct a cutaneous developmental defect. PMID: 12743034
  30. Incubation of mouse macrophages with human laminin alpha5 chain peptide results in marked increase in matrix metalloproteinase-9 mRNA and gelatinolytic activity; this peptide is chemotactic for mouse neutrophils and macrophages in vitro and in vivo. PMID: 12817023
  31. Autocrine LM-5 mediates anchorage-independent survival in breast tumors through ligation of a wild-type, but not a cytoplasmic tail-truncated alpha6beta4 integrin. PMID: 14691145
  32. prostate cancer cells expressing high levels of MT1-MMP have increased invasive potential through their ability to degrade and invade Ln-10 barriers PMID: 15967115
  33. Protein kinase A-dependent phosphorylation of Lutheran/basal cell adhesion molecule glycoprotein regulates cell adhesion to laminin alpha5 PMID: 15975931
  34. An increase was noted in LAMA5 immunostaining in the dilated muscle of the ganglionic bowel upstream the distal aganglionic region in a group of Hirschprung disease patients. PMID: 16226104
  35. Results describe the expression of laminin 5 by parental and c-Ha-ras-transformed HaCaT keratinocytes in organotypic cultures. PMID: 16460839
  36. laminin 5 and p16INK4A have roles in wound healing and senescence PMID: 16723698
  37. The results indicate that glioma cells secrete alpha2-, alpha4- and alpha5-laminins and that alpha3- and alpha5-laminins, selectively promote glioma cell migration and identify alpha3beta1 as the predominant integrin and laminin receptor in glioma cells. PMID: 17888902
  38. alpha5-laminin was the most adhesion- and migration-promoting isoform for human blood lymphocytes, followed by alpha3- (Lm-332) and alpha4- (Lm-411) laminins. PMID: 18523231
  39. These results suggest that laminins containing alpha 5 serve as functional substrates regulating progression of hepatocellular carcinoma. PMID: 18635166
  40. results suggest an evolutionarily conserved role of a member of the laminin gene family in contributing to variation in weight and body composition PMID: 18694491

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Subcellular Location Secreted, extracellular space, extracellular matrix, basement membrane
Tissue Specificity Expressed in heart, lung, kidney, skeletal muscle, pancreas, retina and placenta. Little or no expression in brain and liver.
Database Links

HGNC: 6485

OMIM: 601033

KEGG: hsa:3911

STRING: 9606.ENSP00000252999

UniGene: Hs.473256

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