Human Mast Cell Carboxypeptidase ELISA Kit

Instructions
Code CSB-E13756h
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name carboxypeptidase A3 (mast cell)
Alternative Names carboxypeptidase A ELISA Kit; carboxypeptidase A3 (mast cell) ELISA Kit; Carboxypeptidase A3 ELISA Kit; carboxypeptidase A3 mast cell ELISA Kit; CBPA3_HUMAN ELISA Kit; Cpa3 ELISA Kit; Mast cell carboxypeptidase A ELISA Kit; mast cell carboxypeptidase A3 ELISA Kit; MC CPA ELISA Kit; MC-CPA ELISA Kit; tissue carboxypeptidase A ELISA Kit
Abbreviation CPA3
Uniprot No. P15088
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.312 ng/mL-20 ng/mL
Sensitivity 0.078 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cell Biology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human MC-CPA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %86
Range %80-92
1:2Average %100
Range %94-103
1:4Average %97
Range %94-100
1:8Average %94
Range %86-98
Recovery
The recovery of human MC-CPA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9489-98
EDTA plasma (n=4)9694-98
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
202.477 2.453 2.465 2.366
101.993 1.924 1.959 1.860
51.280 1.271 1.276 1.177
2.50.685 0.678 0.682 0.583
1.250.433 0.424 0.429 0.330
0.6250.286 0.269 0.278 0.179
0.3120.190 0.179 0.185 0.086
00.101 0.097 0.099  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Target Data

Gene References into Functions
  1. Carboxypeptidase A3 was differentially expressed between Fibromyalgia patients and healthy controls. PMID: 27157394
  2. Urine CPA3 was increased in patients with chronic prostatitis/chronic pelvic pain syndrome compared to controls. PMID: 24726923
  3. The cDNA encoding human colon mast cell carboxypeptidase can be successfully cloned and expressed in E. coli and P. pastoris. PMID: 14760754
  4. Role in regulating innate immunity responses and regulating secretory granule homeostasis PMID: 19643669
Subcellular Location Cytoplasmic vesicle, secretory vesicle
Protein Families Peptidase M14 family
Database Links

HGNC: 2298

OMIM: 114851

KEGG: hsa:1359

STRING: 9606.ENSP00000296046

UniGene: Hs.646

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