Human Mast Cell Carboxypeptidase ELISA Kit

Code CSB-E13756h
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P15088
Lead Time 3-5 working days
Abbreviation CPA3
Target Name carboxypeptidase A3 (mast cell)
Alias MC-CPA, carboxypeptidase A3
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.312 ng/mL-20 ng/mL
Sensitivity 0.078 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cell Biology
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details Three different forms of human pancreatic procarboxypeptidase A have been isolated. This gene encodes a form which is obtained as a binary complex of a procarboxypeptidase A with proproteinase E and functions as a secretory granule metalloexopeptidase.
HGNC 2298
RGD 2390
MGI 88479
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human MC-CPA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %86
Range %80-92
1:2Average %100
Range %94-103
1:4Average %97
Range %94-100
1:8Average %94
Range %86-98
Recovery
The recovery of human MC-CPA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9489-98
EDTA plasma (n=4)9694-98
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
202.477 2.453 2.465 2.366
101.993 1.924 1.959 1.860
51.280 1.271 1.276 1.177
2.50.685 0.678 0.682 0.583
1.250.433 0.424 0.429 0.330
0.6250.286 0.269 0.278 0.179
0.3120.190 0.179 0.185 0.086
00.101 0.097 0.099  
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Subcellular Location Cytoplasmic vesicle, secretory vesicle
Protein Families Peptidase M14 family
Database Links

HGNC: 2298

OMIM: 114851

KEGG: hsa:1359

STRING: 9606.ENSP00000296046

UniGene: Hs.646

Pathway Renin-angiotensin system

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