Human Matrix Gla Protein,MGP ELISA Kit

Instructions
Code CSB-E09714h
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
Have Questions? Leave a Message or Start an on-line Chat

Product Details

Description

MGP is a small secretary protein primarily secreted by vascular smooth muscle cells (VSMCs) in the arterial wall. As a potent inhibitor of vascular calcification (VC), MGP exerts calcification inhibition through vitamin K-dependent carboxylation. Additionally, MGP is also involved in fat metabolism and serum inactive MGP level is related to visceral fat.

This assay employs the quantitative sandwich enzyme immunoassay technique. Standards and samples are pipetted into the wells and any MGP present is bound by the anti-human MGP antibody pre-coated onto the microplate. After removing any unbound substances, a biotin-conjugated MGP antibody is added to the wells. After washing, HRP-avidin conjugate is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, the TMB substrate solution is added to the wells and color develops in proportion to the amount of MGP bound in the initial step. The color development is stopped and the intensity of the color is measured.

Target Name matrix Gla protein
Alternative Names Cell growth inhibiting gene 36 protein ELISA Kit; Cell growth-inhibiting gene 36 protein ELISA Kit; GAMMA-CARBOXYGLUTAMIC ACID PROTEIN, MATRIX ELISA Kit; GIG36 ELISA Kit; Matrix Gla protein ELISA Kit; MGLAP ELISA Kit; MGP ELISA Kit; MGP_HUMAN ELISA Kit; NTI ELISA Kit
Abbreviation MGP
Uniprot No. P08493
Species Homo sapiens (Human)
Sample Types serum, plasma, cell culture supernates, tissue homogenates
Detection Range 7.8 pg/mL-500 pg/mL
Sensitivity 1.95 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human MGP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:100Average %100
Range %95-115
1:200Average %97
Range %91-111
1:400Average %97
Range %92-106
1:800Average %93
Range %86-98
Recovery
The recovery of human MGP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9589-98
EDTA plasma (n=4)10196-110
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
5002.712 2.778 2.745 2.641
2502.206 2.231 2.219 2.115
1251.507 1.537 1.522 1.418
62.50.895 0.904 0.900 0.796
31.20.457 0.496 0.477 0.373
15.60.254 0.289 0.272 0.168
7.80.162 0.169 0.166 0.062
00.101 0.106 0.104  
Materials provided
  • A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human MGP antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
  • Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
  • One vial Biotin-labeled MGP antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
  • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
  • One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
  • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
  • One vial Sample Diluent(50 ml/bottle)---Dilute the sample to an appropriate concentration.
  • One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
  • One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
  • One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
  • Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
  • An instruction manual
Materials not provided
  • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
  • An incubator can provide stable incubation conditions up to 37°C±5°C.
  • Centrifuge
  • Vortex
  • Squirt bottle, manifold dispenser, or automated microplate washer
  • Absorbent paper for blotting the microtiter plate
  • 50-300ul multi-channel micropipette
  • Pipette tips
  • Single-channel micropipette with different ranges
  • 100ml and 500ml graduated cylinders
  • Deionized or distilled water
  • Timer
  • Test tubes for dilution
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Earn $30 Amazon Card or 20μL/μg CUSABIO Trial Size Antibody. Details of rewards >>

 Q&A
Q:

On which data are these pre-measurement storage recommendations based?
Can you confirm these storage recommendations also after review?
Other companies describe different storage recommendations. Do you have an idea how these different information concerning the Matrix Gla Protein can occur?

A:
Thanks for your inquiry.
The sample preparation method is based on the consideration of routine biological experiment and the sample degradation.
Generally, if you can use the sample within short-term, pls keep them stored in-20 degrees;
if you will use the sample within long-term, pls keep them stored in -80 degrees.Certainly it is the best to collect the new and fresh sample.
Collecting fresh sample is not easy to realize, so here advice you to keep them stored as our manual. Avoid repeated freeze-thaw and pollution.
As for the other companies mentioned storage, we cannot verify it and no clear advice to provide here.
This kit is used for scientific research and can detect serum plasma sample. Pls check the manual to find the corresponding sample preparation method.
The method is a mature indeed. Of course, it is best to use the newly collected samples for testing.

Target Background

Function
(From Uniprot)
Associates with the organic matrix of bone and cartilage. Thought to act as an inhibitor of bone formation.
Gene References into Functions
  1. Dp-ucMGP was high preoperatively, and further increased postoperatively in cardiovascular risk patients. PMID: 29303985
  2. Study found an association between diastolic left ventricular function with circulating inactive desphospho-uncarboxylated MGP in two cohorts; undertook histological studies confirming the presence of MGP in the heart and determining its exact localization in healthy and diseased hearts, using conformation-specific MGP antibodies. PMID: 29529056
  3. Studied serum levels of bone morphogenic protein-4 (BMP-4) and matrix Gla protein (MGP) in patients who were admitted to emergency department with the diagnosis of acute coronary syndrome (ACS) and underwent primary percutaneous coronary intervention. MGP and BMP-4 levels were significantly elevated when compared to subjects with normal coronary arteries. PMID: 28605143
  4. genetic association studies in population in Greece: Data suggest that an SNP in promoter region of matrix Gla protein (rs1,800,802; T-138C) is associated with diabetic angiopathy (as indicated by carotid intima media thickness) in patients with type 2 diabetes and diabetic nephropathy. PMID: 28734846
  5. This study concluded that diabetes coexisting with renal disease leads to extreme vascular calcification expressed by elevated MGP levels, resulting in higher frequency of cardiovascular disease in comparison to CKD patients without diabetes. PMID: 28654853
  6. Increased desphospho-uncarboxylated matrix Gla protein, which is a circulating biomarker of vitamin K status and vascular calcification, is independently associated with aortic stiffness, but not with stiffness of distal muscular-type arteries. PMID: 26016598
  7. Our results indicate that the association between the matrix Gla protein variant and increased risk for hand Osteoarthritis is caused by a lower expression of matrix Gla protein, which may increase the burden of hand Osteoarthritis by decreased inhibition of cartilage calcification. PMID: 28855172
  8. Genetic variability in the MGP gene was associated with vascular recurrence in the Spanish population. PMID: 28411264
  9. MGP is significantly repressed by miR-155 in breast cancer MCF-7 cells, and concomitantly there is a stimulation of cell proliferation and cell invasiveness. PMID: 27009385
  10. ectopic expression of Mgp in murine and human osteosarcoma cells led to a marked increase in lung metastasis. Abrogation of Mgp prevented lung metastatic activity, an effect that was rescued by forced expression. Mgp levels dramatically altered endothelial adhesion, trans-endothelial migration in vitro and tumour cell extravasation ability in vivo PMID: 27172275
  11. MGP rs4236 [A/G] gene polymorphism was not to be associated with subgingival dental calculus. Also, that GCF MGP levels were detected higher in patients with subgingival dental calculus than those without subgingival dental calculus independently of polymorphism, may be the effect of adaptive mechanism to inhibit calculus formation. PMID: 27348051
  12. The substitution of threonine by alanine due to MGP exon 4 Thr83Ala polymorphism is related to a decrease in the likelihood of arterial calcification in female persons in the Ukrainian population PMID: 27119839
  13. Correlations of plasma desphosphorylated uncarboxylated matrix Gla protein (dp-ucMGP) with vascular calcification and vascular stiffness in chronic kidney disease. Plasma dp-ucMGP was positively associated with vascular calcification and might be utilized as an early marker for vascular calcification in CKD patients. PMID: 27951533
  14. circulating dp-ucMGP and dp-cMGP may have a role in increasing risk of all-cause and cardiovascular mortality PMID: 24835435
  15. It is assumed that Lp-PLA2 is involved in vascular calcification and that dp-ucMGP is a more appropriate biomarker of residual risk than Lp-PLA2 itself. PMID: 25458708
  16. Data shoed increased GAS6 and decreased MGP levels in hemodialysis patients, as mediators of induction or prevention of vascular calcification. PMID: 25957430
  17. Altering NOTCH1 levels affected MGP mRNA and protein. PMID: 25871831
  18. Uncarboxylated MGP in synovial fluid might serve as a novel biomarker for assessing knee osteoarthritis progression. PMID: 26771974
  19. MGP expression is significantly lower in diseased relative to normal aortic valve interstitial cells. Lack of this important "anti-calcification" protein may contribute to calcification of the aortic valve. PMID: 25990696
  20. Higher plasma dephosphorylated uncarboxylated MGP (reflective of lower vitamin K status) was associated with higher odds of meniscus damage, osteophytes, bone marrow lesions, and subarticular cysts. PMID: 25528106
  21. High levels of desphospho-uncarboxylated MGP are independently and positively associated with arterial stiffness after adjustment for common cardiovascular risk factors, renal function, and age. PMID: 25987667
  22. The association has been found between the ischemic atherothrombotic stroke and polymorphic variants of genes MGP and VKORC1. PMID: 26040031
  23. MGP expression is impaired in patients with ankylosing spondylitis PMID: 25974989
  24. The concentration of dephosphorylated-uncarboxylated Matrix Gla protein was higher in hemodialysis patients treated with vitamin K. PMID: 25190488
  25. the findings of this prospective study among type 2 diabetic patients shows that high circulating dpucMGP levels are associated with increased cardiovascular diseases risk, especially with peripheral arterial disease and heart failure. PMID: 23877986
  26. Matrix Gla protein gene functional polymorphisms is associated with loss of bone mineral density and progression of aortic calcification. PMID: 24281054
  27. Inactive nonphosphorylated and uncarboxylated matrix Gla protein levels were followed in a Flemish population. They correlated causally with non-cancer mortality and coronary events but not total cardiovascular mortality. PMID: 25421980
  28. Both mutations predict complete loss of MGP function. PMID: 24458983
  29. High matrix Gla protein levels are associated with below-knee arterial calcification score in type 2 diabetes mellitus patients. PMID: 24762216
  30. Vitamin K insufficiency, as assessed by high plasma matrix Gla protein concentrations, is associated with increased risk for cardiovascular disease independent of classical risk factors and vitamin D status. PMID: 24210635
  31. Based on the study results, the MGP protein did not play an important role in the development of stenosis of coronary arteries. PMID: 24445527
  32. The MGP-138CC genotype may be associated with slower progression of vascular calcification in maintenance hemodialysis patients PMID: 23504408
  33. High MGP concentrations may be associated with more vascular calcification. PMID: 24029658
  34. Higher circulating MGP levels could help identify minor carotid stenosis with calcification in a relatively homogenous risk population (i.e., postmenopausal women), regardless of underlying cardiovascular risk factors PMID: 22992285
  35. The A/A-variant of MGP gene is associated with an increased risk of ischemic atherothrombotic stroke in the Ukrainian women. PMID: 24228496
  36. rs1800802 (T > C) polymorphism within the MGP promoter is not related to stenosis of the coronary artery. PMID: 23563003
  37. Genetic variants of MGP are associated with calcification on the arterial wall. PMID: 23677904
  38. Circulating dp-ucMGP and t-ucMGP may serve as markers for the extent of coronary artery calcification, but these findings need to be confirmed PMID: 22819559
  39. MGP is a multi-functional inhibitor of normal and abnormal angiogenesis that may function by coordinating with both Notch and BMP signaling pathways PMID: 23110920
  40. MGP carboxylation remained much less in pseudoxanthoma elasticum fibroblasts. PMID: 23223140
  41. Menaquinone supplementation dose-dependently decreases dephospho-uncarboxylated MGP concentrations, but does not affect other MGP species. PMID: 23062766
  42. Increased risk of myocardial infarction associated with MGP ThrAla83 genotype observed elsewhere may be related to faster progression of subclinical coronary atherosclerosis. PMID: 23307874
  43. findings showed that SNPrs4236 of the MGP gene is associated with kidney stones in the Chinese Han population, and influences the genetic susceptibility to kidney stones PMID: 23046575
  44. the A/A-variant of MGP gene promotor is associated with an increased risk of ischemic atherothrombotic stroke in female persons in the Ukrainian population. PMID: 23233942
  45. The change in serum fetuin-A, matrix Gla protein (MGP), and osteopontin (OPN) levels after intracerebral hemorrhage (ICH) indicates that these parameters play a role in the pathophysiological processes leading to an ICH. PMID: 22115341
  46. Angiotensin II exacerbates vascular calcification through activation of transcription factors, and regulation of MGP and inflammatory cytokine expression in vascular smooth muscle cells. PMID: 22796540
  47. There may be an association between hand osteoarthritis and genetic polymorphism at the matrix Gla protein (MGP) locus that is not reflected by total MGP serum concentrations. PMID: 21724703
  48. MGP level correlated negatively with proinflammatory cytokines & acute phase proteins in acute pancreatitis, & positively with lipase, fetuin A, & albumin, indicating a possible role in calcium & phosphate metabolism disturbances in AP. PMID: 22239033
  49. a dysregulated MGP system could be involved in left ventricular dysfunction in patients with chronic heart failure PMID: 21294711
  50. Mgp gene deletion may have a role in arteriovenous malformations PMID: 21765215

Show More

Hide All

Involvement in disease Keutel syndrome (KTLS)
Subcellular Location Secreted
Protein Families Osteocalcin/matrix Gla protein family
Database Links

HGNC: 7060

OMIM: 154870

KEGG: hsa:4256

UniGene: Hs.365706

Newsletters

Get all the latest information on Events, Sales and Offers. Sign up for newsletter today.

© 2007-2021 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1