Purity
Greater than 90% as determined by SDS-PAGE.
Research Area
Developmental Biology
Alternative Names
Cell growth inhibiting gene 36 protein; Cell growth-inhibiting gene 36 protein; GAMMA-CARBOXYGLUTAMIC ACID PROTEIN, MATRIX; GIG36; Matrix Gla protein; MGLAP; MGP; MGP_HUMAN; NTI
Species
Homo sapiens (Human)
Expression Region
20-96aa
Target Protein Sequence
YESHESMESYELNPFINRRNANTFISPQQRWRAKVQERIRERSKPVHELNREACDDYRLCERYAMVYGYNAAYNRYF
Note: The complete sequence including tag
sequence, target protein sequence and linker sequence could be provided upon request.
Protein Length
Full Length of Mature Protein
Tag Info
N-terminal 6xHis-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the
glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer,
6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
Amino acids 20-96 form the expressed segment for recombinant Human MGP. The expected molecular weight for the MGP protein is calculated to be 13.5 kDa. This MGP recombinant protein is manufactured in e.coli. The MGP gene fragment has been modified by fusing the N-terminal 6xHis tag, providing convenience in detecting and purifying the recombinant MGP protein during the following stages.
Human matrix gla protein (MGP) is a vital extracellular matrix protein that plays a key role in preventing vascular calcification. Primarily synthesized by vascular smooth muscle cells and chondrocytes, MGP is known for its ability to inhibit the deposition of calcium in soft tissues, including blood vessels and cartilage. As an antagonist of vascular calcification, MGP helps maintain vascular elasticity and integrity. Dysregulation of MGP has been linked to arterial stiffness and cardiovascular diseases. Research on MGP encompasses cardiovascular health, bone metabolism, and tissue mineralization, emphasizing its significance in understanding and potentially treating conditions related to ectopic calcification and maintaining tissue homeostasis.